Ukulandelana okubhaliwe okuphelele - I-Illumina
Izici
● Ilabhulali embaxambili yokulandela i-transcriptome ephelele: Ukulungiswa kweRRNA kulandelwa ngokulungiselela umtapo wezincwadi we-PE150 kanye nokukhethwa kosayizi okulandelwa ngukulungiselela umtapo wezincwadi we-SE50
● Ukuhlaziywa okuphelele kwe-bioinformatics of Mrna, Lncrna, Circkna, Circkna, kanye Mirna kwimibiko ehlukile ye-bioinformatics
● Ukuhlaziywa okuhlangene kwayo yonke inkulumo ye-RNA embikweni ohlanganisiwe, kufaka phakathi ukuhlaziywa kwamanethiwekhi e-cerna.
Izinzuzo zensizakalo
●Ukuhlaziywa okujulile kwamanethiwekhi alawulayo: Ukuhlaziywa kwenethiwekhi ye-Cerna kuvunyelwe ngokulandelana okuhlangene kwe-MRNA, LNCRNA, Circkna, i-Mirna kanye ne-Mirna kanye nokusebenza komsebenzi okuphelele kwe-bioinchatic.
●Isichasiso esibanzi: Sisebenzisa imininingwane eminingi yokufakaza izinhlobo ezivezwe ngokwehluka (ama-degs) nokwenza ukuhlaziya okuhambisanayo kokucebisa, ukuhlinzeka ngemininingwane ngezinqubo zamaselula kanye nama-molecular okuyisisekelo sokuphendula okulandelanayo.
●Ubuchwepheshe obubanzi: Ngerekhodi lethrekhi yokuvala ngempumelelo amaphrojekthi adlule adlule angu-2100 esizindeni esihlukahlukene sokucwaninga, iqembu lethu liletha umcebo wesipiliyoni kulo lonke iphrojekthi.
●Ukulawulwa kwekhwalithi okuqinile: Sisebenzisa amaphuzu alawulwa yi-CORE kuzo zonke izigaba, kusuka kokulungiswa kwesampula kanye nokulungiselela umtapo wezincwadi ukuhlela kabusha kanye ne-bioinformatics. Lokhu kuqapha ngokucophelela kuqinisekisa ukulethwa kwemiphumela esezingeni eliphakeme.
●Ukwesekwa ngemuva kokuthengisa: Ukuzibophezela kwethu kufinyelela ngaphezu kokuphothulwa kwephrojekthi ngesikhashana sesevisi esinenyanga esingu-3. Ngalesi sikhathi, sinikeza ukulandela iphrojekthi, usizo lokuxazulula izinkinga, kanye ne-q & amaseshini ukubhekana nanoma yimiphi imibuzo ehlobene nemiphumela
Izidingo zesampula nokulethwa
| Indlu ehlala izincwadi | Ukuhlehlisa Isu | Kunconywe idatha | Ikhwalithi yokulawula |
| URrna uchithekile | I-Illumina PE150 | I-16 GB | Q3085% |
| Usayizi okhethiwe | I-Illumina Se50 | I-10-20m iyafunda |
Izidingo zesampula:
I-nucleotides:
| Conck. (Ng / μL) | Inani (μg) | Ubumsulwa | Ukwethembeka |
| ≥ 80 | ≥ 1.6 | I-OD260 / 280 = 1.7-2.5 I-OD260 / 230 = 0.5-2.5 Kunomkhawulo noma akukho okungcoliswa kweprotheni noma kwe-DNA ekhonjiswe ku-gel. | Rin Rin8.0 5.00s28s / 18S≥1.0; Kunqunyelwe noma akukho ukuphakama kwesisekelo |
Kunconywe ukulethwa kwezinkampu
Isitsha: 2 ml centrifuge tube (i-tin foil ayinconywa)
Ilebula yesampula: Iqembu + liphindaphinda isib. A1, A2, A3; B1, B2, B3.
Ukuthumela:
1. I-Dry-Ice: Amasampula adinga ukupakishwa ezikhwameni futhi angcwatshwa eqhweni eyomile.
2. Amashubhu angenakuzalwa
Ukugeleza komsebenzi wesevisi
Idizayini yokuhlola
Ukulethwa kwesampula
Ukukhishwa kwe-RNA
Ukwakhiwa Kwelabhulali
Ukulandelana
Ukuhlaziywa kwedatha
Services After-Sale
Bioinformatics
Ukubuka konke kwe-RNA
Uhlobo oluvezwe ngokwehlukile
Ukuhlaziywa kukaCerna
Kuboniswe ngokuhlukile rirnas kanye nama-rnas ahlobene
Hlola intuthuko yocwaningo olwenziwe yi-BMKGENE 'yonke imisebenzi yokulandelana kwayo okulandelayo ngeqoqo lezincwadi ezikhethiwe.
Dai, y. et al. . DOI: 10.1039 / D1MO00370D.
U-Liu, N. nan et al. . Doi: 10.1016 / J.Gene.2022.146503.
Wang, xj et al. . 904865. Doi: 10.3389 / Fonc.2022.904865 / Bibtex.
I-XU, P. et al. . Doi: 10.11186 / S12864-022-08470-3 / Amanani / 7.
Yan, z. Et al. . 111878. I-DoI: 10.1016 / J.Postharvbio.2022.11878.
