I-TGuide S16 Nucleic Acid Extractor
Isingeniso somkhiqizo
I-TGuide S16 Nucleic Acid Extractor ingacubungula amasampuli angafika kwangu-16 ngesikhathi esisodwa ngezinti kazibuthe ukuze ibophe futhi idlulise ubuhlalu kazibuthe, ipuleti elinemithombo engu-96 noma i-cartridge yesampuli eyodwa enesampula kanye nekama lamaphuzu kazibuthe. Ngokusebenzisa i-nucleic acid extraction reagent ehambisanayo, lo mkhiqizo ungahlanza ngokuzenzakalelayo i-DNA/RNA kusukela kumasampuli ahlukahlukene esilwane, isitshalo, amagciwane, isikhunta, njll., futhi ukhiphe i-DNA ku-plasmid, ijeli ye-agarose kanye nomkhiqizo we-PCR.
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Izici
Kulula kakhulu ukusebenzisa
Awukho umsebenzi owengeziwe wamapayipi. Ivolumu ye-elution ingalungiswa njengoba kudingeka.
Ukufakwa nokusebenza kudinga ukuqeqeshwa okuncane. Ngezinhlelo ezifakwe ngaphambilini, qaqa ikhathriji, khetha umthetho olandelwayo bese uqalisa ukuhlola kwakho. Izinhlelo sezilungele ukusetshenziswa, futhi zingenziwa ngendlela oyifisayo.
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Imodi entsha yokubopha
Induku kazibuthe isebenzisa imodi eqinile yokubopha isiphetho.
Ubuhlalu obukazibuthe bubophana ngaphansi kwenduku, okuqinisekisa ukuthi i-eluent isengakwazi ukumboza bonke ubuhlalu kazibuthe ngisho nalapho ivolumu ye-eluent incane. Idizayini ikhulisa isivuno se-nucleic acid.
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Idizayini efanelekile yethangi lokushisa
Kunekholomu efakwe phakathi kwebhafa ye-lysis kanye nekholomu eluent, evimbela ngempumelelo i-eluent ukuthi ingahwamuki ngenxa yokushisisa kwekholomu ye-lysis. Ngakho-ke, umthamo wokubuyisela wesisombululo se-nucleic acid unembe kakhulu.
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Ukulawulwa kokungcola kanye nokuvikela u-opharetha
Ingafakwa ebhentshini elihlanzekile noma ku-hood yamakhemikhali.
Ithuluzi lisebenza ngendlela ezenzakalelayo nevaliwe.
Isistimu yokulawula ukungcola isebenzisa izindlela ezimbili: isistimu yokulawula ukungcoliswa kwangaphakathi kanye nesistimu yokuvala inzalo ye-ultraviolet, ukuze kugwenywe kakhulu ukungcoliswa phakathi kwamakholomu namasampuli.
Ama-reagents alayishwe ngaphambili kanye nezinto ezisetshenziswayo ezilahlwayo.
Ukuphathwa kwamakhemikhali kuqedwa ngezinga elikhulu ukuze kuqinisekiswe ukuphepha kokusebenza.
Imiphumela yedemo
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Umphumela wokuhlola: usebenzisa i-TGuide S16 ene-reagent TGuide Smart Universal DNA Kit (4993704)
ukukhipha ngokuzenzakalelayo i-DNA ye-genomic yamasampuli amaningi ahlanganisa amaseli, izicubu, igazi, indawo yegazi elomile (i-DBS), i-Lactobacillus, i-E.coli, amathe, ne-swab, njll, ukuzuza isivuno esihle kanye nokuhlanzeka okuphezulu.
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I-OD260/OD280 cishe i-1.7~1.9, kanye
OD260/OD230＞1.6.
Umthamo we-Elution: 100 μl
Ukuhlushwa kwejeli ye-Agarose: 1.5%
Ivolumu yokulayisha: 1 μl
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Kusetshenziswa i-TGuide S16 ene-reagent TGuide Smart Viral DNA/RNA Kit (4993702)
ukukhipha ngokuzenzakalelayo i-DNA/RNA emithonjeni eminingi ehlanganisa igazi eliphelele, i-swab kanye ne-plasma kuzuze isivuno esihle nokuhlanzeka okuphezulu. -
I-OD260/OD280 cishe ku-1.7~1.9, kanye ne-OD260/OD230 ~ 1.6.
Inkampani & Inkampani B: Imikhiqizo eyaziwayo.
ND: Isifo saseNewcastle, H5:Umkhuhlane wezinyoni, CP: Canine parvovirus, ASF: African Swine fever virus
Ukuzwela okuncane kungafinyelela ku-100 amakhophi/ml.
Umphumela wokuhlola: ukusebenzisa i-TGuide S16 ene-reagent TGuide Smart Blood Genomic DNA Kit (4993703) ukuze kukhishwe ngokuzenzakalelayo i-DNA ye-genomic yegazi eliqandisiwe kuzuze isivuno esihle kanye nokuhlanzeka okuphezulu, okulingana nesivuno sokukhishwa nobumsulwa bemikhiqizo eyaziwayo eqhudelanayo.
I-OD260/OD280 cishe ku-1.7~1.9, kanye ne-OD260/OD230 ~ 1.7.
Umthombo oyisampula: Amasampula egazi Lomuntu Amathathu. Ukuphindaphinda okuthathu kuwukuhlola kwekhithi ngayinye.
Izimpinda zekhithi ngayinye zisuka emthonjeni wesampula ofanayo.
Isimo sesampula: Igazi eliqandisiwe
Ivolumu yokufaka: 200 μl
Umthamo we-Elution: 100 μl
Ukuhlushwa kwejeli ye-Agarose: 1.5%
Ivolumu yokulayisha: 1 μl
M: Umaka III, TIANGEN
Inkampani & Inkampani B: Imikhiqizo eyaziwayo
2000 bp ingxenye ye-agarose gel DNA ukuhlanzwa kanye nokululama
Ukuhlushwa kwejeli ye-Agarose :1.5%(TBE)
Ivolumu yokulayisha: 8 μl
Umaki: D2000, TIANGEN
I-S16-01, S16-02, kanye ne-S16-03 imelela isampula efanayo yokuhlolwa nokuhlolwa kokutholwa kusetshenziswa i-3 TGuide S16 Nucleic Acid Extractors ene-TGuide Smart DNA Purification Kit (4993550) ngesikhathi esifanayo.
Inkampani: ikhithi yokukhipha imanuwali yekholomu yomkhiqizo owaziwayo
I-Agilent 2100 yasetshenziselwa ukutholwa kobuqotho be-RNA.
Umthombo oyisampula: CD-1 amagundane
Isimo sesampula: igazi elisha eliphelele
Usayizi wesampula: 200 μl
Umphumela wokuhlolwa: isamba se-RNA ekhishwe egazini yi-TGuide S16 ifakwe i-TGuide Smart Blood/Cell/Tissue RNA Kit (4993551) inomkhiqizo omuhle nokuhlanzeka okuphezulu, futhi ingahlangabezana nezidingo zokulandelana komfula osezingeni eliphezulu.
Ivolumu yokulayisha: 1 μl. Isibindi sihlanjululwe izikhathi ezingu-5 futhi salayishwa. Ukuhlushwa kwejeli ye-Agarose: 1%. I-Electrophoresis yemizuzu engu-20 ku-6 V / cm;
M: TIANGEN Umaka III
1-4: inhliziyo 5-8: isibindi 9-12: amaphaphu 13-16: izinso
Amasampuli amabili okuqala esithishu ngasinye akhishwe ikhithi yokukhipha esekelwe kukholomu espin, futhi amabili okugcina akhishwe ngokuzenzakalelayo yi-TGuide S16.
Umthombo oyisampula: CD-1 amagundane
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Umphumela wokuhlolwa: i-DNA yezicubu zesilwane ekhishwe ngokuzenzakalelayo yi-TGuide S16 efakwe i-reagent TGuide Smart Magnetic Tissue DNA Kit (4993547) inomkhiqizo ophezulu nobumsulwa, okulingana ne-DNA ekhishwe yi-spin column-based extraction protocol. Ubumsulwa ngokuyisisekelo buyafana, nge-OD260/OD280 cishe ku-1.7~1.9 kanye ne-OD260/OD230 > 1.7. Sengiphetha, i-TGuide S16 ingasetshenziselwa ukukhipha i-DNA ye-genomic ezicutshini zezilwane esikhundleni sesixazululo sokukhipha esisekelwe kukholomu.
Isamba se-RNA esikhishwe kumasampula ezitshalo ahlukene
Usayizi wesampula: 100 mg
Isampula yokwelashwa kwangaphambili: izinga lokushisa eliphansi le-homogenizer
Ijeli ye-Agarose :1%(TAE)
Ivolumu yokulayisha: 1 μl
M: Umaka III, TIANGEN
1-4: imbewu yommbila 5-8: amaqabunga ommbila 9-12: izimpande zeklabishi 13-16: ubhontshisi wesoya
Amasampuli amabili okuqala akhishwe ngekhithi yokukhipha ikholomu espin futhi amasampula okugcina akhishwe yi-TGuide S16.
Ukukhishwa kwe-Genomic DNA yezimpande zikakolweni
Usayizi wesampula: 100 mg
Ukwelashwa kwangaphambili okuyisampula: ukugaya nge-nitrogen ewuketshezi noma izicubu zokugaya i-homogenizer
Ijeli ye-Agarose :1%(TAE)
Ivolumu yokulayisha: 2 μl
Umaki: D15000, TIANGEN
Inkampani: ibhrendi eyaziwayo
Umphumela wokuhlola: ukusebenzisa i-TGuide S16 ene-reagent TGuide Smart Magnetic Plant DNA Kit (4993548) ukuze kukhishwe ngokuzenzakalelayo i-DNA ye-genomic yezimpande zikakolweni kuzuze isivuno esihle kanye nokuhlanzeka okuphezulu, okulingana nesivuno sokukhipha kanye nobumsulwa besimbangi esaziwayo. Ngaphezu kwalokho, ubuqotho be-DNA ye-genomic bungcono kunemikhiqizo encintisanayo yezinye izinkampani. Kulesi sivivinyo, cishe i-15 μg ye-nucleic acid ikhishwe ezimpandeni zikakolweni ezingu-100 mg, ne-OD260 / OD280 ezungeze i-1.8 ~ 1.9, ne-OD260 / OD230＞2.0.
