I-RNA Encane Ukulandelanisa-Illumina
Izici
● Ukulungiswa komtapo wolwazi kuhlanganisa nesinyathelo sokukhetha usayizi
● Ukuhlaziywa kwe-Bioinformatiki kugxile ekubikezelweni kwe-miRNA kanye nezinhloso zakho
Izinzuzo Zesevisi
●Ukuhlaziywa kwe-bioinformatics okuphelele:Ukunika amandla ukukhonjwa kwakho kokubili ama-miRNA aziwayo kanye namanoveli, ukuhlonzwa kokuhlosiwe kwe-miRNAs, kanye nesichasiselo esisebenzayo esihambisanayo kanye nokunothisa ngemininingwane eminingi (KEGG, GO)
●Ukulawulwa Kwekhwalithi Okuqinile: Sisebenzisa amaphuzu okulawula abalulekile kuzo zonke izigaba, kusukela kusampula nokulungiselela umtapo wezincwadi ukuya ekulandeleni nasekulandeleni i-bioinformatics. Lokhu kuqapha ngokucophelela kuqinisekisa ukulethwa kwemiphumela ehlala njalo yekhwalithi ephezulu.
●Ukusekelwa Kwangemva Kokuthengisa: Ukuzinikela kwethu kudlulela ngalé kokuphothulwa kwephrojekthi ngenkathi yesevisi yezinyanga ezi-3 ngemva kokuthengisa. Ngalesi sikhathi, sinikezela ngokulandelelwa kwephrojekthi, usizo lokuxazulula inkinga, namaseshini e-Q&A ukuze siphendule noma yimiphi imibuzo ehlobene nemiphumela.
●Ubungcweti Olubanzi: Ngomlando wokuvala ngempumelelo amaphrojekthi amaningi e-sRNA ahlanganisa izinhlobo ezingaphezu kuka-300 ezizindeni ezihlukahlukene zocwaningo, ithimba lethu liletha ingcebo yolwazi kuyo yonke iphrojekthi.
Izidingo Zesampula Nokulethwa
| Umtapowolwazi | Inkundla | Idatha enconyiwe | Idatha ye-QC |
| Usayizi ukhethiwe | I-Ilumina SE50 | Ukufundwa kwe-10M-20M | Q30≥85% |
Izimfuneko zesampula:
Ama-Nucleotides:
| Conc.(ng/μl) | Inani (μg) | Ubumsulwa | Ubuqotho |
| ≥80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Amaprotheni anomkhawulo noma awekho nhlobo noma ukungcola kwe-DNA okuboniswa kujeli. | I-RIN≥6.0; 5.0≥28S/18S≥1.0; ukuphakama okulinganiselwe noma okungenakho |
● Izitshalo:
Izimpande, Isiqu noma I-Petal: 450 mg
Iqabunga noma Imbewu: 300 mg
Isithelo: 1.2 g
● Isilwane:
Inhliziyo noma Amathumbu: 450 mg
I-Viscera noma Ubuchopho: 240 mg
Imisipha: 600 mg
Amathambo, Izinwele noma Isikhumba: 1.5g
● Ama-arthropods:
Izinambuzane: 9g
I-crustacea: 450 mg
● Igazi lonke: amashubhu ama-2
● Amaseli: 106 amaseli
● I-Serum ne-Plasma:6 ml
Ukulethwa Kwesampula Okunconyiwe
Isitsha: 2 ml centrifuge tube (Tin foil ayinconywa)
Ukulebula okuyisampula: Ukuphindaphinda kweqembu+isb A1, A2, A3; B1, B2, B3.
Ukuthunyelwa:
1. Iqhwa elomile: Amasampula adinga ukupakishwa ezikhwameni futhi agqitshwe eqhweni elomile.
2. Amashubhu e-RNAstable: Amasampula e-RNA angomiswa ngeshubhu lokuzinzisa le-RNA(isb. i-RNAstable®) futhi athunyelwe ekamelweni lokushisa.
Ukugeleza Komsebenzi Wesevisi
Idizayini yokuhlola
Ukulethwa kwesampula
Ukukhishwa kwe-RNA
Ukwakhiwa komtapo wolwazi
Ukulandelanisa
Ukuhlaziywa kwedatha
Amasevisi angemuva kokuthengisa
I-Bioinformatics
● Ukulawulwa kwekhwalithi yedatha eluhlaza
● ukuhlukaniswa kwe-sRNA
● Ukuqondanisa ku-genome eyireferensi
● Ukuhlonzwa kwe-miRNA eyaziwayo neyenoveli
● Ukuhlaziywa kokuvezwa kwe-miRNA okuhlukile
● Isichasiselo esisebenzayo sokuhlosiwe kwe-miRNA
Ukuhlonzwa kwe-miRNA: isakhiwo nokujula
Ukuvezwa okuhlukile kwe-miRNA - ukuqoqwana kwe-hiearchical
Isichasiselo esisebenzayo sethagethi ye-miRNA evezwe ngokuhlukile
Hlola intuthuko yocwaningo eqhutshwa izinsiza zokulandelana ze-BMKGene' sRNA ngeqoqo elikhethiwe lokushicilelwe.
Chen, H. et al. (2023) 'Ukutheleleka ngegciwane kuvimbela i-saponin biosynthesis kanye ne-photosynthesis e-Panax notoginseng', I-Plant Physiology kanye ne-Biochemistry, i-203, iphe. 108038. doi: 10.1016/J.PLAPHY.2023.108038.
Li, H. et al. (2023) ' Isitshalo se-FYVE esiqukethe iphrotheni FREE1 sihlotshaniswa nezingxenye ze-microprocessor ukucindezela i-miRNA biogenesis ', imibiko ye-EMBO, 24(1). doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.
Yu, J. et al. (2023) 'I-MicroRNA Ame-Bantam-3p Ilawula Ukuthuthukiswa Kwezibungu Zabantu Ngokuqondisa I-Multiple Epidermal Growth Factor-like Domains 8 Gene (megf8) ku-Honeybee, Apis mellifera', Ijenali Yamazwe Ngamazwe Yesayensi Yemolekyuli, 24(6), p. . 5726. doi: 10.3390/IJMS24065726/S1.
U-Zhang, M. et al. (2018) 'Ukuhlaziywa Okudidiyelwe Kwe-MiRNA kanye Nezakhi Zofuzo Ezihambisana Nekhwalithi Yenyama Yembula ukuthi i-Gga-MiR-140-5p Ithinta Ukufakwa Kwamafutha E-Intramuscular Ezinkukhuni', I-Cellular Physiology and Biochemistry, 46(6), pp. 2421-2433. doi: 10.1159/000489649.
