Ukuhlelwa kokulandelana kwe-Bisulfite Yesimangaliso (i-RRBs)
Ukuhlelwa kokulandelana kokumelwa kwe-Bisulfite (ama-RRBs) kuqhamuke njengenye indlela esebenza ngemali futhi esebenza kahle ekubuyiseleni okuphelele kwe-genome bisulfite (i-WGBS) ku-DNA methylation ucwaningo. Ngenkathi i-WGBS ihlinzeka ngemininingwane ephelele ngokuhlola yonke i-genome ekuxazululeni okukodwa, izindleko zayo eziphakeme zingaba yinto ekhawula. I-RRBS ilungisa kahle le nselelo ngokukhetha ingxenye emele ingxenye ye-genome. Le ndlela ithembele ekucebiseni kwezindawo ezicebile ze-CPG yi-MSPI Cleavage yalandelwa izingcezu zobukhulu bezingu-200-5 / 600 BS BS. Ngenxa yalokho, yiminye kuphela izifunda ze-proximal eziqhingini ze-CPG ezihlelwe, kuyilapho lezo ezineziqhingi ezikude ze-CPG zikhishwe ekuhlaziyweni. Le nqubo, ehlanganiswe nokulandelana kwe-Bisulfite, ivumela ukutholwa okuphezulu kwesinqumo se-DNA methylation, kanye nendlela yokulandelana, i-PE150, igxile ngqo emaphethelweni wokufakwa kunokuba maphakathi, okwandisa ukusebenza kwe-methylation profiling. Ama-RRB iyithuluzi elibalulekile elinika amandla ucwaningo lwe-DNA Methylution olungasebenzi kahle futhi luthuthukise ulwazi lwe-Epigenetic Metha.
Izici Zensizakalo
● kudinga i-genome yereferensi.
● I-LambDa DNA isetshenziselwa ukuqapha ukusebenza kahle kwe-Bisulfite.
● Ukusebenza kahle kokugaya kweMSPI kubhekwa futhi.
● Ukugaya okuphindwe kabili kwe-enzyme yamasampula wesitshalo.
● Ukulandelana ku-Illina Novaseq.
Izinzuzo zensizakalo
●Okungabizi kakhulu futhi okusebenzayo kwenye indawo kuma-WGBS: Ukunika amandla ukuhlaziywa kwenziwa ngezindleko eziphansi nangezidingo eziphansi zesampula.
●Isikhulumi Esiphelele:Nikeza ngemisebenzi emihle kakhulu ekucutshungweni kwesampula, ukwakhiwa kwetabhulali, nokulandelana kokuhlaziywa kwe-bioinformatics.
●Ubuchwepheshe obubanzi: Ngemiklamo yokulandelana kwe-RRB yokulandelana iphothule ngempumelelo ezinhlobonhlobo ezahlukahlukene zezinhlobo, i-BMKGENE iletha ngaphezulu kweminyaka eyishumi yokuhlangenwe nakho, iqembu lokuhlaziya elinamakhono kakhulu, okuqukethwe okuphelele, nokusekelwa okuhle kokuthengisa okungcono.
Ukucaciswa Kwensiza
| Indlu ehlala izincwadi | Ukuhlehlisa Isu | Okunconyiwe kwedatha okunconyiwe | Ikhwalithi yokulawula |
| I-MSPI igaywe kanye ne-bisulfite yaphatha umtapo wezincwadi | I-Illumina PE150 | I-8 GB | Q30 ≥ 85% Ukuguqulwa kwe-Bisulfite> 99% Ukusebenza kahle kwe-MSPI> 95% |
Izidingo zesampula
| Ukuhlushwa (ng / μl) | Inani eliphelele (μg) |
| |
| I-genomic DNA | ≥ 30 | ≥ 1 | Ukuwohloka okulinganiselwe noma ukungcoliswa |
Ukugeleza komsebenzi wesevisi
Ukulethwa kwesampula
Ukwakhiwa Kwelabhulali
Ukulandelana
Ukuhlaziywa kwedatha
Services After-Sale
Kubandakanya ukuhlaziywa okulandelayo:
● Ukulawulwa kwekhwalithi okuluhlaza okulula;
● Ukuthola imephu ukuze ubheke i-genome;
● ukutholwa kwezisekelo ze-5mc methylated kanye nokukhonjwa kwe-motif;
● Ukuhlaziywa kokusatshalaliswa kwe-methylation nokuqhathanisa okuyisampula;
● Ukuhlaziywa kwezifunda ezihlukile ze-methylated (DMR);
● Isichasiselo esisebenzayo sezakhi zofuzo ezihambisana ne-DMRS.
Ukulawulwa Kwekhwalithi: Ukusebenza Kokugayeka (kumephu ye-genome)
Ukulawulwa kwekhwalithi: Ukuguqulwa kwe-Bisulfite (ku-methylation infork)
Imephu ye-methylation: 5mc methylation genome-genome-genome
Ukuqhathanisa isampula: Ukuhlaziywa okuyinhloko kwengxenye
Ukuhlaziywa okuhlukile kwe-methylayid (DMRS): Ukushisa kwe-HeatMap
Hlola intuthuko yocwaningo ebika izinsizakalo zokulandelana kwezinsizakalo zeBmkgene zeBorkgene's Genome Bisulfite Services ngeqoqo elikhethiwe lokushicilelwa.
Li, z. et al. (2022) 'Ukuthembeka okuphezulu kabusha kufinyelela kumaseli afana ne-leydig ngokusebenza kwe-crispr kanye nezinto ze-paracrine',Pnas nexus, 1 (4). Doi: 10.1093 / Pnasnexus / PGAC179.
UTian, H. et al. (2023) 'genome-wide dne methylation ukuhlaziya ukwakheka komzimba ngamawele ama-monozygotic ama-Chinese,Ijenali yaseYurophu yophenyo lwasemtholampilo, 53 (11), k. e14055. DOI: 10.11111 / ECI.14055.
I-Wu, Y. et al. (2022) 'I-DNA methylation kanye nesilinganiso se-onistIjenali yophenyo luka-endocrinogical, 45 (12), kk. 2365-2376. Doi: 10.1007 / S40618-022-01878-4.
