Prokaryotic rna ukulandelana
Izici
● Ukucutshungulwa kwesampula ye-RNA kwakubandakanya ukuncipha kwe-RNNA kulandelwa ngokulungiselela umtapo wezincwadi we-RNA.
● Ukuhlaziywa kwe-bioinformatic kususelwa ekuhlelweni kwe-genome yereferensi
● Ukuhlaziywa kufaka phakathi ukuvezwa kofuzo nama-degs kodwa futhi nokwakheka okubhaliwe nokuhlaziywa kwe-SRRA
Izinzuzo zensizakalo
●Ukulawulwa kwekhwalithi okuqinile: Sisebenzisa amaphuzu alawulwa yi-CORE kuzo zonke izigaba, kusuka kokulungiswa kwesampula kanye nokulungiselela umtapo wezincwadi ukuhlela kabusha kanye ne-bioinformatics. Lokhu kuqapha ngokucophelela kuqinisekisa ukulethwa kwemiphumela esezingeni eliphakeme.
●Idatha yokulandelana eqondene ne-Strand: Ngenxa yokulungiselela umtapo wezincwadi we-RNA Ukuba yinkohliso, kunika amandla ukubhekisisa okubhaliwe kwe-anti -bery.
●Ukuhlaziywa okuphelele kwenziwa ku-Prokaryotic Transcripts: Ipayipi le-bioinformatic lifaka ukuhlaziya okuwukuhlaziywa kwezakhi zofuzo kuphela kepha futhi nokuhlaziywa kwesakhiwo esibhaliwe, kufaka phakathi ukuhlonza ama-aconcring, UTS kanye nabagqugquzeli. Kufaka nokuhlaziywa kwe-SRNAS, okungukuthi isichasiso nokubikezela kwesakhiwo sesibili namatshe okuhlosiwe.
●Ukwesekwa ngemuva kokuthengisa: Ukuzibophezela kwethu kufinyelela ngaphezu kokuphothulwa kwephrojekthi ngesikhashana sesevisi esinenyanga esingu-3. Ngalesi sikhathi, sinikezela ngokulandela iphrojekthi, usizo lokuxazulula inkinga, kanye ne-q & amaseshini ukubhekana nanoma yimiphi imibuzo ehlobene nemiphumela.
Izidingo zesampula nokulethwa
| Indlu ehlala izincwadi | Ukuhlehlisa Isu | Kunconywe idatha | Ikhwalithi yokulawula |
| I-RRNA IQHAWE LOKUQHAWULA KAKHULU | I-Illumina PE150 | 1-2 gb | Q3085% |
Izidingo zesampula:
| Conck. (Ng / μL) | Inani (μg) | Ubumsulwa | Ukwethembeka |
| ≥ 50 | ≥ 1 | I-OD260 / 280 = 1.8-2.0 I-OD260 / 230 = 1.0-2.5 Kunomkhawulo noma akukho okungcoliswa kweprotheni noma kwe-DNA ekhonjiswe ku-gel. | Qhina.5 |
Kunconywe ukulethwa kwezinkampu
Isitsha: 2 ml centrifuge tube (i-tin foil ayinconywa)
Ilebula yesampula: Iqembu + liphindaphinda isib. A1, A2, A3; B1, B2, B3.
Ukuthumela:
1. I-Dry-Ice: Amasampula adinga ukupakishwa ezikhwameni futhi angcwatshwa eqhweni eyomile.
2. Amashubhu angenakuzalwa
Ukugeleza komsebenzi wesevisi
Ukulethwa kwesampula
Ukwakhiwa Kwelabhulali
Ukulandelana
Ukuhlaziywa kwedatha
Services After-Sale
Ukuhlaziywa kokuhlaziywa kwe-Bioinformatic
Kubandakanya ukuhlaziywa okulandelayo:
● Ukulawulwa kwekhwalithi yedatha eluhlaza
● Ukuhlangana kwe-genome yereferensi
● Ukuhlolwa kwekhwalithi yeLabhulali: ukungahleliwe kwe-RNA Fgvermentation, faka usayizi nokulandelana kokulandelana
● Isichasiselo esisebenzayo sezinhlobo ezibikezelwe zamakhodi
● Ukuhlaziywa Kwezimo: Ukuxhumeka kanye nokuhlaziywa okuyinhloko kwengxenye (i-PCA)
● I-gene gene ehlukile (ama-degs)
● Isichasiselo esisebenzayo kanye nokucebisa ama-degs
● Ukuhlaziywa kwe-SRNA
● Ukuhlaziywa Kwesakhiwo Sokubhala: Izisebenzi, Ukuqala kanye Nezikhundla Zokugcina, Isifunda Esingelonakalisiwe (UTS), Ukuphakanyiswa kwe-SNP / Indel
Ukulandelana ngokugcwala
Isichasiselo esisebenzayo sezinhlobo zamakhodi
Ukuhlangana phakathi kwamasampula
Ukuhlaziywa okuhlukile kokuchazwa (degs)
Ukuhlaziywa kokucebisa okusebenzayo
Isichasiso se-SRRA
Hlola intuthuko eyenziwe nge-Bmkgene's Nanopore ebunjiswe ama-nanopore izinsizakalo zokulandelana kwe-Mrna kulolu shicilelo olufakiwe.
Guan, CP et al. .I-Polish Journal of Microbiology, 67 (2), k. 223. Doi: 10.21307 / PJM-2018-024.
