-
Imitapo yolwazi eyenziwe ngaphambilini ye-DNBSEQ
I-DNBSEQ, ethuthukiswe yi-MGI, ubuchwepheshe be-NGS obukwazile ukwehlisa ngokuqhubekayo izindleko zokulandelana nokwandisa ukuphuma. Ukulungiswa kwemitapo yolwazi ye-DNBSEQ kuhilela ukuhlukaniswa kwe-DNA, ukulungiswa kwe-ssDNA, kanye nokukhulisa indilinga ukuze kutholwe ama-nanoballs e-DNA (DNB). Lezi zibe sezilayishwa endaweni eqinile bese zilandelana ngokulandelana nge-Probe-Anchor Synthesis (cPAS). Ubuchwepheshe be-DNBSEQ buhlanganisa izinzuzo zokuba nezinga lephutha lokukhulisa eliphansi nokusebenzisa amaphethini wamaphutha wokuminyana okuphezulu nama-nanoballs, okuholela ekulandeleni ngokuphuma okuphezulu nokunemba.
Isevisi yethu yokulandelanisa umtapo wolwazi eyenziwe ngaphambilini yenza amakhasimende akwazi ukulungiselela imitapo yolwazi yokulandelana kwe-Illumina evela emithonjeni ehlukahlukene (mRNA, i-genome yonke, i-amplicon, imitapo yolwazi eyi-10x, phakathi kokunye), eguqulelwa kumalabhulali we-MGI ezindaweni zokucwaninga zethu ukuze ihlelwe ku-DNBSEQ-T7, ivumela amanani edatha aphezulu ngezindleko eziphansi.
-
Imitapo yolwazi eyenziwe nge-Illumina
Ubuchwepheshe bokulandelana kwe-Illumina, obusekelwe ku-Sequencing by Synthesis (SBS), iwukuqanjwa okusha kwe-NGS okwamukelwa emhlabeni wonke, okubophezelekile ukukhiqiza ngaphezu kuka-90% wedatha yokulandelana komhlaba. Umgomo we-SBS ubandakanya ukuthwebula izithombe ezishintshashintshayo ezilebulwe ngokushintshashintsha kwe-fluorescent njengoba i-dNTP ngayinye yengezwa, futhi kamuva igoqe ukuze kuvunyelwe ukufakwa kwesisekelo esilandelayo. Ngawo wonke ama-dNTP amane abophezelayo abophezelayo akhona kumjikelezo ngamunye wokulandelana, ukuncintisana kwemvelo kunciphisa ukuchema kokufakwa. Lobu buchwepheshe obuhlukahlukene busekela imitapo yolwazi efundwa kanye kanye naleyo ebhanqiwe, ihlinzekela uhla lwezinhlelo zokusebenza ze-genomic. Amakhono we-Illumina wokulandelanisa okuphuma phambili nokunemba akubeka njengesisekelo ocwaningweni lwe-genomics, kunikeza ososayensi amandla okuqaqa ubunkimbinkimbi bezakhi zofuzo ngemininingwane engenakuqhathaniswa nokusebenza kahle.
Isevisi yethu yokulandelanisa umtapo wolwazi eyenziwe ngaphambilini yenza amakhasimende akwazi ukulungisa amalabhulali alandelanayo avela emithonjeni eyahlukene (mRNA, i-genome yonke, i-amplicon, imitapo yolwazi engu-10x, phakathi kokunye). Ngokulandelayo, le mitapo yolwazi ingathunyelwa ezikhungweni zethu zokulandelanisa ukuze zilawule ikhwalithi nokulandelana ezisekelweni ze-Illumina.
