-
I-PacBio-Full-length 16S/18S/ITS Amplicon Sequencing
Iplathifomu ye-Amplicon (16S/18S/ITS) yathuthukiswa yaba neminyaka yokuhlangenwe nakho ekuhlaziyweni kwephrojekthi ye-microbial diversity, equkethe ukuhlaziya okuyisisekelo okujwayelekile kanye nokuhlaziya komuntu siqu: ukuhlaziya okuyisisekelo kuhlanganisa okuqukethwe kokuhlaziya okujwayelekile kocwaningo lwamanje lwe-microbial, okuqukethwe kokuhlaziya kucebile futhi kubanzi, futhi imiphumela yokuhlaziya yethulwa ngendlela yemibiko yephrojekthi; Okuqukethwe kokuhlaziywa komuntu siqu kuhlukahlukene. Amasampuli angakhethwa futhi amapharamitha angasethwa ngendlela evumelana nezimo ngokuvumelana nombiko wokuhlaziya oyisisekelo kanye nenjongo yocwaningo, ukuze kufezeke izidingo eziqondene nawe. Isistimu yokusebenza yeWindows, elula futhi esheshayo.
-
I-PacBio-Full-length Transcriptome (Non-Reference)
Ithatha idatha yokulandelanisa ye-Pacific Biosciences (PacBio) Isoform njengokufakwayo, lolu hlelo lokusebenza luyakwazi ukubona ukulandelana kokulotshiweyo kobude obugcwele (ngaphandle kokuhlanganisa). Ngokwenza imephu ukulandelana kobude obugcwele ngokumelene ne-reference genome, okulotshiweyo kungenziwa ngcono ngezakhi zofuzo ezaziwayo, imibhalo, izifunda zekhodi, njll. Kulokhu, ukuhlonza okunembe kakhudlwana kwezakhiwo ze-mRNA, njengokunye ukuhlanganisa, njll, kungafinyelelwa. Ukuhlaziywa okuhlangene nedatha yokulandelanisa ye-NGS transcriptome kunika amandla isichasiselo esibanzi kanye nokulinganisa okunembe kakhudlwana ekukhulumeni ezingeni lokuloba, okuzuzisa kakhulu ukuvezwa komehluko ongezansi nokuhlaziywa kokusebenza.
-
Amathuluzi
I-BMKCloud iyinkundla ehamba phambili ye-bioinformatic ehlinzeka ngesixazululo esisodwa-stop sezinhlelo ze-genomic, ezethenjwa kakhulu abacwaningi emikhakheni eyahlukene okuhlanganisa ezokwelapha, ezolimo, ezemvelo, njll. I-BMKCloud izibophezele ekuhlinzekeni ngezinsizakalo ezididiyelwe, ezinokwethenjelwa neziphumelelayo ezihlanganisa izinkundla zokuhlaziya i-bioinformatic namathuluzi. , izinsiza zekhompuyutha, isizindalwazi somphakathi, izifundo ze-inthanethi ze-bioinformatic, njll. I-BMKCloud inamathuluzi ahlukahlukene asetshenziswa njalo e-bioinformatic afaka isichasiselo sofuzo, amathuluzi ofuzo okuziphendukela kwemvelo, I-ncRNA, ukulawulwa kwekhwalithi yedatha, ukuhlanganisa, ukuqondanisa, ukukhishwa kwedatha, ukuguqulwa kwezakhi zofuzo, izibalo, i-generator yezibalo, ukuhlaziya ukulandelana, njll.
-
I-RNA encane
Ama-RNA amancane awuhlobo lwe-RNA emfushane engafaki ikhodi enobude obuyisilinganiso esingu-18-30 nt, okuhlanganisa i-miRNA, i-siRNA ne-piRNA. Lawa ma-RNA amancane abikwe kakhulu ukuthi ahileleke ezinqubweni ezihlukahlukene zebhayoloji ezifana nokuwohloka kwe-mRNA, ukuvimbela ukuhumusha, ukwakheka kwe-heterochromatin, njll. Ukuhlaziywa kokulandelana kwe-SmallRNA kusetshenziswe kabanzi ezifundweni zokuthuthukiswa kwezilwane/izitshalo, izifo, igciwane, njll. I-RNA encane. inkundla yokuhlaziya ukulandelana iqukethe ukuhlaziywa okujwayelekile kanye nokumbiwa kwedatha okuthuthukisiwe. Ngokwesisekelo sedatha ye-RNA-seq, ukuhlaziya okujwayelekile kungafinyelela ukuhlonza nokubikezela kwe-miRNA, ukubikezela kofuzo okuhlosiwe kwe-miRNA, isichasiselo nokuhlaziywa kwenkulumo. Ukuhlaziywa okuthuthukile kuvumela ukusesha nokukhipha i-miRNA ngendlela oyifisayo, ukukhiqizwa komdwebo we-Venn, i-miRNA kanye nokwakhiwa kwenethiwekhi yofuzo oluqondiwe.
-
I-NGS-WGS (Illumina/BGI)
I-NGS-WGS iyinkundla yokuhlaziya yokulandelanisa kabusha kofuzo lonke, ethuthukiswe kusisekelo solwazi olucebile ku-Biomarker Technologies. Le nkundla yokusebenziseka kalula ivumela ukuthunyelwa okusheshayo kokugeleza komsebenzi okuhlanganisiwe kokuhlaziya ngokumane kusethe ipharamitha eyisisekelo embalwa, elingana nedatha yokulandelana kwe-DNA ekhiqizwe kusuka kokubili iplathifomu ye-Illumina kanye neplathifomu yokulandelana kwe-BGI. Le nkundla isetshenziswa kuseva yekhompuyutha esebenza kahle, enika amandla ukuhlaziywa kwedatha okusebenza kahle kakhulu ngesikhathi esilinganiselwe kakhulu. Ukumbiwa kwedatha yomuntu siqu kuyatholakala kusisekelo sokuhlaziya okujwayelekile, okuhlanganisa umbuzo wofuzo oguquliwe, idizayini yokuqala ye-PCR, njll.
-
mRNA(Inkomba)
I-Transcriptome isixhumanisi phakathi kolwazi lofuzo lwe-genomic kanye ne-proteome yomsebenzi webhayoloji. Ukulawulwa kwezinga lokubhalwa kwemibhalo kuyindlela yokulawula ebaluleke kakhulu futhi efundwa kabanzi kakhulu yezinto eziphilayo. Ukulandelana kwe-Transcriptome kungalandelanisa i-transcriptome nganoma yisiphi isikhathi noma ngaphansi kwanoma yisiphi isimo, ngesinqumo esinembile ku-nucleotide eyodwa. isampula yokuloba ethize.
Njengamanje, ubuchwepheshe bokulandelana kwe-transcriptome busetshenziswe kabanzi ku-agronomy, imithi kanye neminye imikhakha yocwaningo, okuhlanganisa ukulawulwa kokuthuthukiswa kwezilwane nezitshalo, ukujwayela imvelo, ukusebenzisana kwamasosha omzimba, ukutholakala kwezakhi zofuzo, ukuguquguquka kofuzo kanye nokutholwa kwezifo zofuzo.
-
I-Metagenomics (NGS)
Le nkundla yokuhlaziya yakhelwe ukuhlaziya idatha ye-shotgun metagenomic ngokusekelwe eminyakeni yolwazi. Iqukethe ukuhamba komsebenzi okuhlanganisiwe okuqukethe ukuhlaziya okuhlukahlukene okuvame ukudingeka kwe-metagenomics okuhlanganisa ukucutshungulwa kwedatha, izifundo zezinga lezinhlobo zezilwane, izifundo zezinga lokusebenza kwezakhi zofuzo, ukuhlanganisa i-metagenome, njll. Ngaphezu kwalokho, amathuluzi okumba idatha enziwe ngokwezifiso ayatholakala ekuhlaziyweni okujwayelekile kokuhlaziywa, okuhlanganisa umbuzo wofuzo kanye nezinhlobo zezilwane. , ukulungiselelwa kwepharamitha, ukukhiqiza izibalo eziqondene nawe, njll.
-
I-LncRNA
Ama-RNA amade angafaki amakhodi (lncRNA) awuhlobo lwemibhalo enobude obude kuno-200 nt, engakwazi ukufaka ikhodi amaprotheni. Ubufakazi obuningi buphakamisa ukuthi iningi lama-lncRNA kungenzeka ukuthi asebenze. Ubuchwepheshe bokulandelana komkhiqizo ophezulu kanye namathuluzi okuhlaziya nge-bioinformatic kusinikeza amandla okuveza ukulandelana kwe-lncRNA nolwazi lokubeka kahle kakhudlwana futhi kusiholele ekutholeni ama-lncRNA anemisebenzi ebalulekile yokulawula. I-BMKCloud iyaziqhenya ngokunikeza amakhasimende ethu inkundla yokuhlaziya ukulandelana kwe-lncRNA ukuze afinyelele ukuhlaziya kwe-lncRNA okusheshayo, okuthembekile nokuguquguqukayo.
-
I-GWAS
Ucwaningo lwe-Genome-wide association (GWAS) luhlose ukuhlonza okuhlukile kofuzo (i-genotype) ehlotshaniswa nezici ezithile (i-phenotype). Ucwaningo lwe-GWA luphenya izimpawu zofuzo eziwela lonke i-genome yenani elikhulu labantu futhi zibikezela izinhlangano ze-genotype-phenotype ngokuhlaziywa kwezibalo ezingeni labantu. Ukulandelanisa kabusha kwezakhi zofuzo kungathola zonke izinhlobo zofuzo. Ngokuhambisana nedatha ye-phenotypic, i-GWAS ingacutshungulwa ukuze kuhlonzwe ama-SNP ahlobene ne-phenotype, ama-QTL kanye nezakhi zofuzo zamakhandidethi, okusekela ngokuqinile ukuzalaniswa kwezilwane/izitshalo kwesimanje. I-SLAF isu lokulandelanisa i-genome elenziwe lula, elithola izimpawu ezisatshalaliswe ngofuzo olubanzi, i-SNP. Lawa ma-SNP, njengama-molecular genetic markers, angacutshungulwa ukuze kuhlangatshezwane nezifundo nezici ezihlosiwe. Kuyisu elingabizi kakhulu ekuhlonzeni izici eziyinkimbinkimbi ezihlobene nokuhlukahluka kofuzo.
-
I-Nanopore Ubude obugcwele be-transcriptomics
Ama-isoform ayinkimbinkimbi futhi aguquguqukayo ezinto eziphilayo ayizindlela ezibalulekile zofuzo zokulawula ukubonakaliswa kofuzo nokuhlukahluka kwamaprotheni. Ukuhlonza okunembile kwezakhiwo zokulotshwa kuyisisekelo socwaningo olujulile lwamaphethini okulawula isisho sofuzo. Inkundla yokulandelana ye-Nanopore ilethe ngempumelelo isifundo se-transcriptomic ezingeni le-isoform. Le nkundla yokuhlaziya iklanyelwe ukuhlaziya idatha ye-RNA-Seq ekhiqizwe kuplathifomu ye-Nanopore kusisekelo se-reference genome, ezuza ukuhlaziya okunekhwalithi nokwenani kukho kokubili izinga lofuzo kanye nezinga lokulotshwa.
-
isiyingi-RNA
I-RNA eyindilinga(circRNA) iwuhlobo lwe-RNA engafakwanga ikhodi, esanda kutholwa inendima ebalulekile kumanethiwekhi okulawula ahilelekile ekuthuthukisweni, ukumelana nemvelo, njll. Ihlukile kuma-molecule e-RNA aqondile, isb mRNA, lncRNA, 3′ kanye no-5′. iziphetho ze-circRNA zihlanganiswe ndawonye ukuze zenze isakhiwo esiyindilinga, esiwasindisa ekugayweni kwe-exonuclease futhi azinze kakhulu kuneningi i-RNA yomugqa. I-CircRNA itholwe inemisebenzi eyahlukahlukene ekulawuleni ukubonakaliswa kofuzo. I-CircRNA ingenza njenge-ceRNA, ebopha i-miRNA ngokuncintisana, eyaziwa ngokuthi isipontshi se-miRNA. Inkundla yokuhlaziya ukulandelana kwe-CircRNA inika amandla ukwakheka kwe-circRNA nokuhlaziywa kwenkulumo, ukubikezela okuhlosiwe nokuhlaziya okuhlangene nezinye izinhlobo zama-molecule e-RNA.
-
BSA
Iplathifomu yokuhlaziywa kwe-Bulked Segregant iqukethe ukuhlaziya okujwayelekile okuyisinyathelo esisodwa kanye nokuhlaziya okuthuthukile ngokulungiselelwa kwepharamitha engokwezifiso. I-BSA yindlela esetshenziswa ukuhlonza ngokushesha izimpawu zofuzo ezihlobene ne-phenotype. Ukuhamba komsebenzi okuyinhloko kwe-BSA kuqukethe: 1. ukukhetha amaqembu amabili abantu abane-phenotypes ephikisana kakhulu; 2. ukuhlanganisa i-DNA, i-RNA noma i-SLAF-seq(Ithuthukiswe yi-Biomarker) yabo bonke abantu ukuze bakhe inqwaba ye-DNA; 3. ukuhlonza ukulandelana okuhlukile ngokumelene ne-reference genome noma phakathi, 4. ukubikezela ikhandidethi izifunda ezixhunywe nge-ED kanye ne-SNP-index algorithm; 5. Ukuhlaziywa kokusebenza kanye nokunothisa izakhi zofuzo ezifundeni zamakhandidethi, njll. Ukumbiwa kwezimayini okuthuthuke kakhulu kudatha okuhlanganisa nokuhlolwa komaka wofuzo kanye nomklamo wokuqala nakho kuyatholakala.
-
I-Amplicon (16S/18S/ITS)
Iplathifomu ye-Amplicon (16S/18S/ITS) yathuthukiswa yaba neminyaka yokuhlangenwe nakho ekuhlaziyweni kwephrojekthi ye-microbial diversity, equkethe ukuhlaziya okuyisisekelo okujwayelekile kanye nokuhlaziya komuntu siqu: ukuhlaziya okuyisisekelo kuhlanganisa okuqukethwe kokuhlaziya okujwayelekile kocwaningo lwamanje lwe-microbial, okuqukethwe kokuhlaziya kucebile futhi kubanzi, futhi imiphumela yokuhlaziya yethulwa ngendlela yemibiko yephrojekthi; Okuqukethwe kokuhlaziywa komuntu siqu kuhlukahlukene. Amasampuli angakhethwa futhi amapharamitha angasethwa ngendlela evumelana nezimo ngokuvumelana nombiko wokuhlaziya oyisisekelo kanye nenjongo yocwaningo, ukuze kufezeke izidingo eziqondene nawe. Isistimu yokusebenza yeWindows, elula futhi esheshayo.
