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I-BMKMANU S3000_Spatial Transcriptome
I-Spatial transcriptomics imi phambili ekusungulweni kwesayensi, inika amandla abacwaningi ukuthi bahlole amaphethini enkulumo eyinkimbinkimbi yezakhi zofuzo phakathi kwezicubu kuyilapho belondoloza isimo sabo sendawo. Phakathi kwamapulatifomu ahlukahlukene, i-BMKGene ithuthukise i-BMKManu S3000 Spatial Transcriptome Chip, eziqhayisa ngokulungiswa okuthuthukisiwe kwe-3.5µm, efinyelela ububanzi obuncane, futhi ivumela izilungiselelo zokuxazulula amazinga amaningi. I-chip ye-S3000, equkethe cishe izindawo eziyizigidi ezi-4, isebenzisa ama-microwell agqitshwe ngobuhlalu obulayishwe ama-probe wokuthwebula anebhakhodi. Umtapo wezincwadi we-cDNA, ohlanganiswe namabhakhodi wendawo, ulungiswa kusukela ku-chip ye-S3000 bese ulandelana ngokulandelana endaweni ye-Illumina NovaSeq. Inhlanganisela yamasampula anebhakhodi yendawo kanye nama-UMI kuqinisekisa ukunemba nokucaciswa kwedatha ekhiqizwayo. I-chip ye-BMKManu S3000 iguquguquka kakhulu, inikezela ngezilungiselelo zokuxazulula amazinga amaningi ezingashunwa kahle kumathishu ahlukene kanye namazinga afiswayo emininingwane. Lokhu kuzivumelanisa nezimo kubeka i-chip njengenketho evelele yezifundo ezihlukahlukene ze-spatial transcriptomics, iqinisekisa ukuhlangana okunembayo kwendawo okunomsindo omncane. Ukusetshenziswa kobuchwepheshe bokuhlukanisa amaseli nge-BMKManu S3000 kuvumela ukunqunywa kwedatha yokubhala emikhawulweni yamaseli, okuholela ekuhlaziyweni okunencazelo eqondile yebhayoloji. Ngaphezu kwalokho, ukulungiswa okuthuthukisiwe kwe-S3000 kuphumela enanini eliphezulu lezakhi zofuzo kanye nama-UMI atholiwe ngeseli ngalinye, okuvumela ukuhlaziya okunembe kakhulu kwamaphethini okulotshwa kwendawo nokuhlanganisa amaseli.
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Imitapo yolwazi eyenziwe ngaphambilini ye-DNBSEQ
I-DNBSEQ, ethuthukiswe yi-MGI, ubuchwepheshe be-NGS obukwazile ukwehlisa ngokuqhubekayo izindleko zokulandelana nokwandisa ukuphuma. Ukulungiswa kwemitapo yolwazi ye-DNBSEQ kuhilela ukuhlukaniswa kwe-DNA, ukulungiswa kwe-ssDNA, kanye nokukhulisa indilinga ukuze kutholwe ama-nanoballs e-DNA (DNB). Lezi zibe sezilayishwa endaweni eqinile bese zilandelana ngokulandelana nge-Probe-Anchor Synthesis (cPAS). Ubuchwepheshe be-DNBSEQ buhlanganisa izinzuzo zokuba nezinga lephutha lokukhulisa eliphansi nokusebenzisa amaphethini wamaphutha wokuminyana okuphezulu nama-nanoballs, okuholela ekulandeleni ngokuphuma okuphezulu nokunemba.
Isevisi yethu yokulandelanisa umtapo wolwazi eyenziwe ngaphambilini yenza amakhasimende akwazi ukulungiselela imitapo yolwazi yokulandelana kwe-Illumina evela emithonjeni ehlukahlukene (mRNA, i-genome yonke, i-amplicon, imitapo yolwazi eyi-10x, phakathi kokunye), eguqulelwa kumalabhulali we-MGI ezindaweni zokucwaninga zethu ukuze ihlelwe ku-DNBSEQ-T7, ivumela amanani edatha aphezulu ngezindleko eziphansi.
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I-Hi-C esekelwe ku-Chromatin Interaction
I-Hi-C iyindlela edizayinelwe ukuthwebula ukucushwa kwe-genomic ngokuhlanganisa ukuhlola okususelwe ekubeni seduze kanye nokulandelana komphumela ophezulu. Indlela isekelwe ekuxhumaneni kwe-chromatin ne-formaldehyde, okulandelwa ukugayeka kokudla kanye nokuhlanganisa kabusha ngendlela yokuthi izingcezu kuphela ezixhunywe ngokuhlanganyela zizokwakha imikhiqizo yokuhlanganisa. Ngokulandelanisa le mikhiqizo ye-ligation, kungenzeka ukufunda inhlangano ye-3D ye-genome. I-Hi-C inika amandla ukufunda ngokusatshalaliswa kwezingxenye zegenome ezipakishwe kancane (Amakhompathimenti A, i-euchromatin) futhi okungenzeka zisebenze ngokubhalwa phansi, nezifunda ezipakishwe kakhulu (amakhompathimenti B, i-Heterochromatin). I-Hi-C ingase futhi isetshenziselwe ukukhomba izizinda ezihlotshaniswa ne-Topologically Associated Domains (TADs), izifunda zofuzo ezinezakhiwo ezigoqiwe futhi okungenzeka zibe namaphethini wokukhuluma afanayo, kanye nokuhlonza amaluphu e-chromatin, izifunda ze-DNA ezihlanganiswe ndawonye ngamaphrotheni futhi evame ukucetshiswa ezintweni zokulawula. Insizakalo yokulandelana ye-Hi-C ye-BMKGene inika abacwaningi amandla okuhlola ubukhulu bendawo be-genomics, bavule izindlela ezintsha zokuqonda ukulawulwa kofuzo kanye nomthelela wako kwezempilo nezifo.
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I-TGuide Smart Magnetic Plant RNA Kit
I-TGuide Smart Magnetic Plant RNA Kit
Hlanza isamba sekhwalithi ephezulu ye-RNA ezicutshini zezitshalo
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I-TGuide Smart Blood/Cell/Tissue RNA Kit
I-TGuide Smart Blood/Cell/Tissue RNA Kit
Ikhithi egcwaliswe ngaphambili ye-cartridge / plate reagent yokuhlanzwa kwesivuno esikhulu, ukuhlanzeka okuphezulu, i-RNA ephelele, yekhwalithi ephezulu, engena-inhibitor evela esicutshini sesilwane/iseli/igazi eliphelele
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I-TGuide Smart Magnetic Plant DNA Kit
I-TGuide Smart Magnetic Plant DNA Kit
Hlanza i-DNA ye-genomic yekhwalithi ephezulu kusuka ezicutshini zezitshalo ezahlukahlukene
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TGuide Smart Soil / Stool DNA Kit
TGuide Smart Soil / Stool DNA Kit
Ihlanza i-DNA engena-inhibitor yokuhlanzeka okuphezulu nekhwalithi kusuka kumasampula enhlabathi nasezindle
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TGuide Smart DNA Purification Kit
Ibuyisela i-DNA yekhwalithi ephezulu evela kumkhiqizo we-PCR noma amajeli e-agarose.
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I-TGuide Smart Blood Genomic DNA Kit
I-TGuide Smart Blood Genomic DNA Kit
Ikhithi egcwaliswe ngaphambili ye-cartridge / plate reagent yokuhlanzwa kwe-DNA ye-genomic egazini nejazi le-buffy
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I-TGuide Smart Magnetic Tissue DNA Kit
Ikhithi egcwaliswe ngaphambili ye-cartridge / plate reagent yokukhipha i-genomic DNA ezicutshini zezilwane
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I-TGuide Smart Universal DNA Kit
Ikhithi egcwaliswe ngaphambili ye-cartridge / plate reagent yokuhlanza i-genomic DNA egazini, indawo yegazi elomile, amagciwane, amaseli, amathe, ama-swabs omlomo, izicubu zezilwane, njll.
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I-TGuide S16 Nucleic Acid Extractor
I-TGuide S16 Nucleic Acid Extractor
I-Benchtop Instrument esebenziseka kalula, amasampula ayi-1-8 noma ayi-16 ngasikhathi sinye
Inombolo yekhathalogi / iphakheji
Ikati. cha
ID
Inombolo ye-preps
I-OSE-S16-AM
1 isethi
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I-PacBio 2+3 Isixazululo Sobude obugcwele be-mRNA
Ngenkathi ukulandelana kwe-mRNA okususelwe ku-NGS kuyithuluzi eliguquguqukayo lokulinganisa ukusho kofuzo, ukuthembela kwayo ekufundeni okufushane kuvimbela ukusebenza kwayo ekuhlaziyeni okuyinkimbinkimbi kwe-transcriptomic. Ngakolunye uhlangothi, i-PacBio sequencing (Iso-Seq) isebenzisa ubuchwepheshe obufundwe isikhathi eside, ivumela ukulandelana kwemibhalo yobude obugcwele ye-mRNA. Le ndlela isiza ukuhlola okuphelele kokunye ukuhlanganisa, ukuhlanganisa izakhi zofuzo, kanye ne-poly-adenylation, nakuba kungeyona inketho eyinhloko yokulinganisa isisho sofuzo. Inhlanganisela engu-2+3 ivala igebe phakathi kwe-Illumina ne-PacBio ngokuthembela ekufundweni kwe-PacBio HiFi ukuze kuhlonzwe isethi ephelele yama-isoform abhaliwe kanye nokulandelana kwe-NGS ukuze kulinganise ama-isoform afanayo.
Amapulatifomu: I-PacBio Sequel II/ PacBio Revio kanye ne-Illumina NovaSeq;
