Isitshalo / isilwane sonke se-genome
Izici Zensizakalo
● Ukulungiselela umtapo wezincwadi kungaba ngcono noma nge-PCR-free
● Kutholakala kumapulatifomu wokulandelana 4 wokulandelana: I-Illina NovaseQ, MGI T7, i-Nanopore Promethotion P48, noma i-Pacbio Revuo.
● Ukuhlaziywa kwe-bioinformatic kugxile ekutholeni okuhlukahlukene: I-SNP, Indel, SV ne-CNV
Izinzuzo zensizakalo
●Ubuchwepheshe obubanzi namarekhodi okushicilela: Okuhlangenwe nakho okuqoqiwe ekulandeleni kwe-genome kwezinhlobo ezingaphezu kuka-1000 kuholele emacaleni angaphezu kwe-1000 ashicilelwe ngesici sethonya elibandayo labantu abangaphezu kuka-5000.
●Ukuhlaziywa okuphelele kwe-bioinformatics: Kubandakanya ukuguquguquka kokushintshana kanye nesichasiso somsebenzi.
● Ukwesekwa kwangemva kokuthengisa:Ukuzibophezela kwethu kunwebeka ngaphezu kokuphothulwa kwephrojekthi ngesikhashana sezinyanga ezi-3 ngemuva kokuthengisa. Ngalesi sikhathi, sinikezela ngokulandela iphrojekthi, usizo lokuxazulula inkinga, kanye ne-q & amaseshini ukubhekana nanoma yimiphi imibuzo ehlobene nemiphumela.
●Isichasiso esibanzi: Sisebenzisa ama-databases amaningi ukuze akwazisa ngokusebenza kwezakhi zofuzo ngokuhlukahluka okuhlonziwe futhi enze ukuhlaziya okuhambisanayo kokucebisa, ukuhlinzeka ngemininingwane kumaphrojekthi wokucwaninga amaningi.
Ukucaciswa Kwensiza
| Ukuhlukahluka okuzokhonjwa | Ukuhlehlisa Isu | Ukujula okunconyiwe |
| I-SNP ne-Indel | I-Illumina Novaseq PE150 noma i-MGI T7 | Eliyo |
| I-SV ne-CNV (inembile kangako) | I-30x | |
| I-SV ne-CNV (kunembe kakhudlwana) | I-Nanopore Prom P48 | 20x |
| I-SNPS, Indels, SV ne-CNV | I-Pacbio Revuo | Eliyo |
Izidingo zesampula
| Izicubu noma ama-nucleic acid ama-nucleic | I-Illumina / MGI | I-nanopore | Pacbio
| ||
| Isilwane se-viscera | 0.5-1 g | ≥ 3.5 g
| ≥ 3.5 g
| ||
| Izicubu zezilwane | ≥ 5 g
| ≥ 5 g
| |||
| Igazi LaseMammalian | 1.5 ml | ≥ 0.5 ml
| ≥ 5 ml
| ||
| I-PoulTry / Igazi Lezinhlanzi | ≥ 0.1 ml
| ≥ 0.5 ml
| |||
| Isitshalo- iqabunga elisha | 1-2 g | ≥ 2 g
| ≥ 5 g
| ||
| Amaseli abunjiwe |
| ≥ 1x107
| ≥ 1x108
| ||
| Izicubu ezithambile zezinambuzane / umuntu ngamunye | 0.5-1 g | ≥ 1 g
| ≥ 3 g
| ||
| Kukhishwe i-DNA
| Ukugxila: ≥ 1 ngl Inani: ≥ 30 ng Kukhawulelwe noma akukho ukucekelwa phansi noma ukungcoliswa
| Ukuqoqana Inani
I-OD260 / 280
I-OD260 / 230
Kukhawulelwe noma akukho ukucekelwa phansi noma ukungcoliswa
| ≥ ≥ ng / μl 4 μg / ukugeleza iselula / isampula
1.7-2.2
≥1.5 | Ukuqoqana Inani
I-OD260 / 280
I-OD260 / 230
Kukhawulelwe noma akukho ukucekelwa phansi noma ukungcoliswa | ≥ ≥ ng / μl I-10 μg / i-Flow Cell / Isampula
1.7-2.2
1.8-2.5 |
| Ukulungiselela umtapo wamahhala kwe-PCR-Free: Okuhlaziyelayo 40 ng / μl Inani 500 ng | |||||
Ukugeleza komsebenzi wesevisi
Ukulethwa kwesampula
Ukukhishwa kwe-DNA
Ukwakhiwa Kwelabhulali
Ukulandelana
Ukuhlaziywa kwedatha
Ukulethwa Kwedatha
Kubandakanya ukuhlaziywa okulandelayo:
- Ukulawulwa kwekhwalithi yedatha eluhlaza
- Izibalo zokuqondanisa ukuze zibhekwe i-genome genome
- Ukuhlonza okuhlukile: I-SNP, Indel, SV ne-CNV
- Isichasiselo esisebenzayo sokuhlukahluka
Izibalo zokuqondanisa ukuze zibhekwe i-genome yereferensi - Ukulandelana kokusatshalaliswa kokujula
I-SNP ibiza phakathi kwamasampula amaningi
Ukukhonjwa kwe-Indel - Izibalo zobude be-Indel esifundeni se-CDS kanye nesifunda sase-Genome-Wide
Ukusatshalaliswa okuhlukile kulo lonke uhlobo lwe-genome - uzungu lwe-circos
Isichasiso esisebenzayo sezakhi zofuzo ngokuhlukahluka okuhlonziwe - i-gene ontology
I-Chai, Q. et al. . 433. Doi: 10.11111 / PBI.13965.
UCheng, H. et al. (2023) 'I-Chromosome-Level Wild Hevea Brasiliensis Geneasiensis Genes inikeza amathuluzi amasha okuzala afungelwe kwezolona kanye ne-lociate ebalulekile ukukhulisa isivuno senjoloba', isitshalo se-biotechnology Journal, 21 (5), PP. 1058-1072. DOI: 10.11111 / PBI.14018.
Li, a. et al. . DOI: 10.1038 / S42003-021-02823-6.
Zeng, T. et al. (2022) Ukuhlaziywa kwe-genome ne-methylation inkukhuna yendabuko yaseChinese ngokuhamba kwesikhathi kuhlinzeka ngokuqonda ngezinhlobo zezilwane ezilondoloziwe ', i-5 (1), i-PP. 1-12. Doi: 10.1038 / S42003-022-03907-7.
