I-Pacbio yobude obugcwele be-transcriptome

I-PacBio Full-Length Transcriptome Sequencing, i-Isoseq, inika amandla ukukhonjwa okunembile kwama-isoforms abhaliwe, kukhanyisela enye i-polyadenylation kanye nokuhlanganisa, futhi kuholele ekuhlaziyweni okunembe kakhudlwana kwesisho sofuzo. Ipayipi le-BMKCloud PacBio Lobude obugcwele be-Transcriptome yakhelwe ukuhlaziya imitapo yolwazi ye-cDNA elandelana ngemodi ye-Circular Consensus Sequencing (CCS) futhi ihlonze ukulandelana kwe-Full-Length Non-Chimeric (FLNC), ebese ihlanganiswa ibe imibhalo engadideki. Ukuhlaziywa okulandelayo kwe-BUSCO kuhlola ukuphelela komhlangano we-transcriptome. Kusuka ku-transcriptome ehlanganisiwe, ukuhlaziya okuningi kuyenziwa: okunye ukuhlukaniswa, ukuphindaphinda kokulandelana okulula (SSR), ukubikezela kwe-lncRNA kanye nezakhi zofuzo eziqondiwe ezihambisanayo, ukubikezelwa kwezakhi zofuzo zenoveli, ukuhlaziywa komndeni wofuzo, ukuhlaziywa kwesici sokuloba, kanye nesichasiselo esisebenzayo semibhalo ebhaliwe.