I-Genome Evolution
I-pnas
Umsuka wokuziphendukela kwemvelo kanye nomlando osezingeni eliphezulu we-Goldfish (Carassius auratus)
Pacbio | I-Illumina | I-Bionano Genome Imephu | Sawubona-C I-Genome Assembly | Imephu yofuzo | I-GWAS | I-RNA-SEQ
Amaphuzu Avelele
I-1.goldfish genome yavuselelwa ngenguqulo yomhlangano osezingeni eliphakeme, i-anchroring 95.75% yama-concrigs ibe yi-50 pseudochromosomes (scaffold N50 = 31.84 MB). Amabanga amabili angaphansi adilizwa.
Izifunda ze-2.Genomic zokushanela okukhethiwe ngesikhathi sokuhlala zikhonjwe ekuvuseleleni idatha yango-201 abantu, ukwembulela izakhi zofuzo ezingaphezu kuka-390 ezihlotshaniswa nezimfanelo zoMnyango.
I-3.GWas kwi-Dorsal Fin e-Goldfish ye-Goldfish yaveza izakhi zofuzo ezingama-378 ezihlotshaniswa. Intatheli ye-tyrosine-protein yesiKinase ikhonjwe njenge-gene yokhetho oluhambisana nokusobala
Isendlalela
I-Goldfish (Carassius auratus) ingenye yezinhlanzi ezibaluleke kakhulu ezipulazi, ezazisezindlini ze-Crucian Carp eChina yasendulo. Baphawulwe nguCharles Darwin ngokuthi "ukudlula ngaphezulu kokuhlukahluka kombala okungekho emthethweni, sihlangana nokuguqulwa okumangalisa kakhulu kwesakhiwo". Izici ezihluke kakhulu nomlando omude wokuhlala phansi nokuzalanisa Yenza i-bullifish enhle yohlelo lwemodeli yofuzo lwe-Fish Physiology kanye nokuziphendukela kwemvelo.
Impumelelo
Goldfish Genme
JUkuhlaziywa kuka-Oint ye-PACBIO ne-Illina Pair-End Sequencing Idatha iveza umhlangano wokuqala we-1.657 g (i-ContiG N50 = 474 KB). I-Bionano Optical Map yakhiqizwa futhi yalungisa umhlangano ku-1.73 GB ngosayizi (usayizi we-genome olinganisiwe: 1.8 GB). I-HI-C ESEBULEKILE EMVELWE ISCAFFDD N50 yasuka ku-606 KB kuya ku-31.84 MB futhi yathola ama-95.75% (1.65 GB) athambekele futhi ayale amanani e-anchling. I-genome iqukethe izinhlobo ezingama-56,251 zokubhala amakhodi kanye nombhalo omude ongewona amakhodi ayi-10,098. Ngaphezu kwalokho, izifunda ezingama-38 ezingaba khona zeCentromeric zabikezelwa ngama-chromosomes angama-50.
Fig.1 Igolide lezinhlanzi genome
TKukhonjwe amasethi aphansi ama-subgegendes kuma-chromosomes angama-50 wegolide aphumela emcimbini wasendulo wokuhlanza. Iqoqo lama-chromosomes anengxenye ephakeme yokufundwa okuqondaniwe phakathi kwe-goldfish kanye ne-barbanae achazwa njenge- (Chra01 ~ A25), okungukuthi okuvamile njenge-barbinae, kanye nokusalayo njenge-subgegenome B (CHB01 ~ B25).
Ukuhlala phansi kanye nokukhethwa okukhethiwe
AIngqikithi yezinkomba eziyi-16 zohlobo lwe-ruc crucian kanye nokwehluka okumele kwegolide zegolide kwavuselelwa ngokulandelana okuphakathi kokulandelana kokulandelana kokunye kokuhlehlisa okungama-12,5x, okudala ama-turebases ayi-4,3. Ukuhlaziywa kokwakhiwa kabusha kwe-phylogenetic kanye ne-PCA kuqinisekisile ukuhlangana okusondele phakathi kwegolide elijwayelekile kanye ne-Crucian Carp kunenye i-Goldfish, okwahlukaniswa izimbotshana ezimbili.
LD Ukuhlaziywa kokubola ngenhla kwama-subpopation amane akwesekele ubukhona be-geneneneck yabo ye-geneneck ngesikhathi sokukhetha kanye nokukhethwa okuqinile kokufakelwa ku-Goldfish. Ukwanda kokuhlukahluka kofuzo (π) kusuka ku-Crucian Carp ku-Wordfish evamile ku-Wen Goldfish futhi i-eggyfish yegolide kuveze ukunqwabelana okubalulekile kokuhlukahluka kofuzo ngesikhathi sokuhlukahluka kwabo. Izindawo ezikhethiwe ezingama-50 ezimboza izifunda ezingama-25 ze-genomic ezimboza i-25.2 MB no-946 izinhlobo zofuzo zitholakale kwimininingwane yokumele (33 yegolide kanye ne-16 crucian crap). Ukunwebeka kokuhlaziywa kwabanye abangu-201, izinhlobo ezingama-393 ezikhonjisiwe ezifundeni zokulondolozwa okuqediwe. Lezi zitho zofuzo zatholakala zihluka kakhulu, okungenzeka zaba nomthelela ezimisweni ezihambisana nezimfanelo ezinkulu ze-goldfish.
I-Fig.3 Ukuhlaziywa okuhlobene nokuhlaziywa kwezindawo ezihlobene nodokotela
I-GWAS KWI-Goldfish Yezindlu
DI-Orsal Fin isici esiyisihluthulelo esihlukanisa i-Wen Goldfish kusuka kwi-Egg Goldfish. I-GWAS ye-Dorsal Fin ku-96 Wen Goldfish kanye ne-87 ye-Goldfish ye-Worldfish iveze ukuthi izakhi zofuzo ezingama-378 zokhetho zasakazeka ngaphesheya kwama-chromosomes ayi-13 kanye nokusatshalaliswa okungalingani kwalezi zigaba phakathi kwalezi zifuzo eziphakathi kwaphansi phakathi kwalezi zangaphansi phakathi kwaphansi. Ukuhlaziywa okusebenzayo kuzakhitho zokhetho kuqokomise izinqubo zemvelo kufaka phakathi "ukusayina kwe-cell Super Receptor", "Transmemmembrane Trastistation", "Skeletal System Development", njll.
I-Fig.4 Gwas ye-Dorsal Fin ku-Goldfish yasekhaya
In Gwas yezimpawu ezihlobene nokukali okusobala, kutholwe i-association eyodwa enamandla. Uhlobo lwe-gene efaka ikhodi ye-tytrosine-protein enase receplor kukhonjwe kwelinye lezona ezifundela ukhetho.
I-Fig.5 GWAS yezimpawu ezihlobene nokukalibala
Inkomba
Chen d et al. Umsuka wokuziphendukela kwemvelo kanye nomlando wokuhlanya kwezindawo ezindlini zegolide (Carassius auratus). I-PNAS (2020)
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Isikhathi sePosi: Jan-04-2022