I-Nanopore-based Full-length Transcriptome Sequencing
Ukulandelana kwe-Nanopore kuzihlukanisa kwezinye izinkundla zokulandelana, ngokuthi ama-nucleotide afundwa ngokuqondile ngaphandle kokuhlanganiswa kwe-DNA futhi akhiqize ukufunda okude emashumini ama-kilobase. Lokhu kunika amandla ukufundwa okuqondile kokulotshiweyo kobude obugcwele kanye nokubhekana nezinselele ezifundweni ze-transcriptomic.
√ Ukuchema okuqondene nokulandelana okuphansi
√ Ukufundwa kobude obugcwele kwe-cDNA kuya ezifundweni zesakhiwo sofuzo
√ Idatha encane edingekayo ukumboza inombolo efanayo yemibhalo ebhaliwe
√ Ukukhonjwa kwama-isoform amaningi isakhi ngasinye
√ Ukulinganisa kwenkulumo kuzinga le-isoform
Ipayipi le-Bioinformatics elijwayelekile
Amacala Aphumelele Akamuva Nge-Biomarker Technologies
1. Ukuhlaziya okuqhathanisayo kwama-transcriptomes anobude obugcwele kuveza ukuguquguquka kwe-Gnetum luofuense stem
Ijenali: Frontiers in Genetics
Kushicilelwe: Mashi 2021
Amagama angukhiye: Minion | Okunye ukuhlanganisa | APA | lncRNA | Okulotshiweyo okuvezwe ngokwehlukile
Funda Umbhalo Ogcwele
2. Ukunyakaza okukodwa kwama-RNA amancane ukusuka emahlumela kuya ezimpandeni kuma-interspecific heterografts
Ijenali: Izitshalo Zemvelo
Kushicilelwe: Jan. 2021
Amagama angukhiye: Nanopore | Ilumina | ukutholwa kwe-mRNA yeselula yobude obugcwele
3. Ukuhlaziywa kwe-transcriptome yobude obugcwele bezimpande ze-asparagus kuveza indlela yamangqamuzana yokubekezelela usawoti okubangelwa isikhunta se-arbuscular mycorrhizal.
Ijenali: Isayensi Yezemvelo Nokuhlola
Kushicilelwe: Jan. 2021
Amagama angukhiye: Minion | Imibhalo ebhalwe ngokwehlukile | Izici zokuloba | Okunye ukuhlanganisa
4. Izinguquko Zesimo Semvelo kanye Nombhalo Obhalwe Ngobude Obugcwele Bezimpande (Rosa chinensis) Izimpande Namaqabunga Ekuphenduleni Kukucindezeleka Kwesomiso
Ijenali: I-Plant and Cell Physiology
Kushicilelwe: Okthoba 2020
Amagama angukhiye: PromethION | Imibhalo ebhalwe ngokwehlukile | Isibikezelo esisebenzayo | Ama-isoform ahlukanisiwe | I-TF kanye ne-lncRNA
5. Ukuhlaziywa nokuqhathanisa okuphelele kwe-PacBio kanye nokulandelana kwe-RNA okusekelwe ku-nanopore kwe-Arabidopsis transcriptome
Ijenali: Indlela Yezitshalo
Kushicilelwe: Ephreli 2020
Amagama angukhiye: Igridi | PromethION | I-PacBio Sequel | Illumina NovaSeq | Ukuqondanisa kwe-genome | Umazisi wombhalo | Okunye ukuhlanganisa | SSR | LncRNA | I-isoform quantification
Izindaba Namaphuzu Avelele ihlose ukwabelana ngamacala akamuva ayimpumelelo ne-Biomarker Technologies, ithwebula izimpumelelo zesayensi ezinoveli kanye nezindlela ezivelele ezisetshenziswe phakathi nocwaningo.
Isikhathi sokuthumela: Jan-08-2022