I-GENOME
Ukuhlanganiswa kwesikali se-Chromosome nokuhlaziywa kwesitshalo se-biomass Miscanthus lutarioriparius genome
Ukulandelana kweNanopore | Ilumina | Hi-C | Ukulandelana kwe-RNA | I-Phylogeny
Kulolu cwaningo, i-Biomarker Technologies inikeze ukusekelwa kwezobuchwepheshe ekulandeleni kwe-Nanopore, umhlangano we-de novo genome, umhlangano osizwa yi-Hi-C, njll.
Abstract
I-Miscanthus, isitshalo se-rhizomatous perennial, sinamandla amakhulu okukhiqizwa kwe-bioenergy ngenxa ye-biomass ephezulu kanye nokubekezelela ingcindezi. Sibika ukuhlangana kwesikali se-chromosome kweI-Miscanthus lutarioripariusi-genome ngokuhlanganisa ukulandelana kwe-Oxford Nanopore kanye nobuchwepheshe be-Hi-C. Umhlangano we-2.07-Gb uhlanganisa i-96.64% ye-genome, ne-contig N50 ye-1.71 Mb. Ukulandelana kwe-centromere ne-telomere kuhlanganiswe kuwo wonke ama-chromosome angu-19 kanye ne-chromosome 10, ngokulandelana. Umsuka we-Allotetraploid we-M. lutarioriparius uqinisekiswa kusetshenziswa ukuphindaphinda kwesathelayithi eyi-centromeric. Isakhiwo se-tetraploid genome kanye nokuhlelwa kabusha kwechromosomal okuningana okuhlobene namabele kuboniswa ngokucacile. Izakhi zofuzo eziyimpinda ye-Tandem yeM. lutarioripariusUkusebenza akuthuthukisiwe kuphela ngokwemibandela ehlobene nokusabela kwengcindezi, kodwa ne-cell wall biosynthesis. Imindeni yofuzo ehlobene nokumelana nezifo, i-cell wall biosynthesis kanye nokuthuthwa kwe-ion yensimbi yandiswa kakhulu futhi yavela. Ukwandiswa kwale mindeni kungase kube isisekelo esibalulekile se-genomic sokuthuthukisa izici eziphawulekayo zeM. lutarioriparius.
Izibalo ezibalulekile zokuhlanganisa i-genome
Umfanekiso. Uhlolojikelele lwe-M. lutarioriparius genome assembly
Izindaba Namaphuzu Avelele ihlose ukwabelana ngamacala akamuva ayimpumelelo ne-Biomarker Technologies, ithwebula izimpumelelo zesayensi ezinoveli kanye nezindlela ezivelele ezisetshenziswe phakathi nocwaningo.
Isikhathi sokuthumela: Jan-05-2022