I-Nanopore Ubude obugcwele be-transcriptome

Ukulandelana kwe-Nanopore transcriptome kuyindlela enamandla yokulandelanisa ama-cDNAs anobude obugcwele, ukuhlonza ngokunembile kanye nokulinganisa ama-isoform alotshiweyo. I-BMKCloud Nanopore Full-Length Transcriptome Pipeline iklanyelwe ukuhlaziya idatha ye-RNA-Seq ekhiqizwe endaweni yesikhulumi se-Nanopore ngokumelene ne-genome yereferensi yekhwalithi ephezulu enezichasiselo ezinhle, ehlinzeka ngokuhlaziywa kwekhwalithi nokwamanani kukho kokubili izinga lofuzo nelombhalo. Ngemuva kokulawula ikhwalithi, ukulandelana okugcwele okungeyona kwe-chimeric (FLNC) ubude obugcwele kuyatholwa futhi ukulandelana kwemvumelwano kufakwa imephu ku-genome eyireferensi ukuze kususwe imibhalo engasasebenzi. Kusukela kule sethi yokulotshiweyo, isisho silinganiswa futhi izakhi zofuzo ezivezwa ngokuhlukile futhi okulotshiweyo kuyahlonzwa futhi kuchazwe ngokusebenza. Ipayipi liphinde lihlanganise okunye ukuhlaziywa kwe-polyadenylation (APA), okunye ukuhlaziywa kokuhlukanisa, ukuhlaziya okuphindaphindwayo kokulandelana okulula (SSR), ukubikezela kwe-lncRNA nokuhlosiwe okuhambisanayo, ukubikezela kokulandelana kwekhodi (CDS), ukuhlaziywa komndeni wofuzo, ukuhlaziywa kwesici sokuloba, ukubikezelwa kwezinhlobo ezintsha zofuzo. kanye nesichasiselo esisebenzayo semibhalo elotshiwe.