mRNA-seq (NGS) – De novo

Ukulandelana kwe-mRNA kuvumela ukuphrofayilila kwakho konke okulotshiwe kwe-mRNA kumaseli ngaphansi kwezimo ezithile futhi kuwubuchwepheshe obusetshenziswa kakhulu ezindaweni ezihlukahlukene zocwaningo. Ipayipi le-BMKCloud De novo mRNA-seq yakhelwe ukuhlaziya imitapo yolwazi yokulandelana ethuthukisiwe ye-poly-A lapho kungekho genome eyinkomba etholakalayo. Ipayipi iqala ngokulawulwa kwekhwalithi, kulandelede novoukuhlanganisa okulotshiweyo nokukhethwa kwesethi ye-unigene. Ukuhlaziywa kwesakhiwo se-unigene kubikezela ukulandelana kwekhodi (CDS) nokuphindaphinda ukulandelana okulula (SSR). Ngokulandelayo, ukuhlaziywa kwesisho esihlukile kuthola izakhi zofuzo ezivezwe ngokuhlukile (ama-DEG) phakathi kwezimo ezihloliwe, kulandelwa isichasiselo esisebenzayo nokunothisa kwama-DEG ukuze kukhishwe imininingwane yebhayoloji.