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Imikhiqizo

I-Hi-C esekelwe eChromatin Ukusebenzisana

I-Ch-C esekelwe kwi-Chromatin Intanethi efakiwe

I-HI-C iyindlela eyenzelwe ukuthwebula ukucushwa kwe-genemic ngokuhlanganisa ukusebenzisana okususelwa ekuphakamiseni nokulandelana okuphezulu kokudluliswa. Indlela isuselwa ku-Chromatin iwela nge-formaldehyde, ilandelwe ukugaya kanye nokuphinda i-ligation ngendlela okuhlangene kuphela okuxhunyaniswe yimikhiqizo ye-ligation. Ngokulandelana le mikhiqizo ye-ligation, kungenzeka ukutadisha inhlangano ye-3D ye-genome. I-HI-C Ivumela ukutadisha ukusatshalaliswa kwezingxenye ze-genome ezigcwele kancane (izingxenye, i-euchromatin) futhi amathuba okuthi akhuthele ngokwethuthi, futhi izifunda, i-heterochromatin). I-HI-C nayo ingasetshenziswa ukukhomba izizinda ezihambisana ne-topologited (ama-tads), izifunda ze-genome ezinamaphethini asongelayo futhi zithola amaphethini okubonisana okufanayo, futhi ukuthola izingodo ze-chromatin, izifunda ze-DNA ezihlotshiswe ngamaprotheni futhi lokho kuvame ukucebisa ezintweni zokulawula. I-Bmkgene's Hi-C Ukulandelanisa Insizakalo inika abaphenyi ukuthi bahlole ubukhulu bendawo ye-genomics, ukuvula izindlela ezintsha zokuqonda ukutholwa kwe-genome kanye nemithelela yayo kwezempilo nezifo.

Xhumana nathi

Imininingwane ye-Service

Bioinformatics

Imiphumela yedemo

Izincwadi Ezifakiwe

Izici Zensizakalo

● Ukulandelana ku-Illumina Novaseq nge-PE150.

● Insizakalo idinga amasampula wezicubu, esikhundleni sokukhipha ama-nucleic acid, ukuwela isixhumanisi nge-formaldehyde futhi kulondolozwe ukusebenzisana kwamaprotheni we-DNA.

● Isilingo se-HI-C sifaka ukunqunyelwa kanye nokuphetha ukulungiswa kwemiphetho enamathelayo nge-biotin, kulandelwa ukujikeleza kwezinjongo eziphumayo ze-blunt ngenkathi kulondolozwa ukusebenzisana. I-DNA bese idonswa phansi nge-streptavidin ubuhlalu futhi ihlanzwe ngokulungiselela umtapo wezincwadi okulandelayo.

Izinzuzo zensizakalo

●Idizayini ekhawulelwe ye-enzyme: Ukuqinisekisa ukusebenza kahle kwe-HI-C ephezulu kwezinhlobo ezahlukene ezinama-93% ama-run avumelekile wokuxhumana.

●Ubuchwepheshe obubanzi namarekhodi okushicilela:I-BMKGENE inesipiliyoni esikhulu nge-2 2000 hi-c ukulandelana kwamaphrojekthi kusuka ezinhlotsheni ezahlukahlukene ezingama-800 namalungelo obunikazi ahlukahlukene. Amacala angaphezu kwe-100 ashicilelwe ngesici sokuqongelela esingaphezu kuka-900.

●Iqembu elinekhono elinamakhono amakhulu we-bioinformatics:Ngamalungelo obunikazi e-In-House kanye nama-copyrights wesoftware wokuhlolwa kwe-Hi-C nokuhlaziywa kwedatha kanye nesoftware yedatha yokuzibonakalisa.

●Ukusekelwa kokuthengisa ngemuva:Ukuzibophezela kwethu kunwebeka ngaphezu kokuphothulwa kwephrojekthi ngesikhashana sezinyanga ezi-3 ngemuva kokuthengisa. Ngalesi sikhathi, sinikezela ngokulandela iphrojekthi, usizo lokuxazulula inkinga, kanye ne-q & amaseshini ukubhekana nanoma yimiphi imibuzo ehlobene nemiphumela.

●Isichasiso esibanzi: Sisebenzisa ama-databases amaningi ukuze akwazisa ngokusebenza kwezakhi zofuzo ngokuhlukahluka okuhlonziwe futhi enze ukuhlaziya okuhambisanayo kokucebisa, ukuhlinzeka ngemininingwane kumaphrojekthi wokucwaninga amaningi.

Ukucaciswa Kwensiza

Indlu ehlala izincwadi

Ukuhlehlisa Isu

Okunconyiwe kwedatha okunconyiwe

Isixazululo sesiginali se-Hi-C

I-Hi-C LibraryL Library

I-Illumina PE150

I-Chromatin Loop: 150x

I-TAD: 50x

I-Chromatin Loop: 10KB

I-TAD: 40KB

Izidingo Zensiza

Uhlobo lwesampula	Inani elidingekayo
Izicubu zezilwane	≥2G
Igazi lonke	≥2ml
Isilingi mp3	≥1g
Izitshalo- izicubu ezincane	I-1G / Aliquot, 2-4 Aliquotes Kunconyiwe
Amaseli abunjiwe	≥1x10⁷

Okwedlule: Tguide smart magnetic Plant RNA KINIT

Olandelayo: Imitapo yolwazi yangaphambi kwe-DNBSEQ

Kubandakanya ukuhlaziywa okulandelayo:

● Idatha eluhlaza qc;

● Imephu kanye ne-Hi-C Library Library QC: Amabili ukusebenzisana kwababili kanye nokuxhumana kokubola

● Ukuxhumana okubanzi kwe-Genome-Okubanzi

● Ukuhlaziywa kokusatshalaliswa kweqomela le-A / B;

● Ukukhonjwa kwama-TADS nama-chromatin loops;

● Ukuhlaziywa okwehlukene kwizakhi zesakhiwo ze-chromatin ze-chromatin phakathi kwamasampula kanye nesichasiselo esisebenzayo sezakhi zofuzo ezihambisanayo.

CIS NOKUQHAWULA ISIQINISEKISO SOKUQHAWULA