Ukuhlaziywa kwe-Eukaryotic mRNA (Okukhethwa kukho okusekelwe kanye ne-de novo kuyatholakala)
Leli payipi lisebenzisa idatha ye-NGS RNA-Seq njengokufakwayo futhi likhiqiza imiphumela evela ekuhlaziyweni okuningi komfula okuhlanganisa kodwa okungakhawulelwe kulokhu: ukulandelana kokuhlolwa kwekhwalithi yedatha,de novoIsichasiselo sesayithi lokulotshiweyo, ukuhlaziywa kokuhlukanisa okuguquguqukayo, ukuhlaziya isisho esihlukanisayo, isichasiselo somsebenzi nokuhlaziywa kokunothisa.
Ukuhlaziywa kwe-RNA okude okungabhali ikhodi
Ama-RNA amade angafaki amakhodi (lncRNA) ayimibhalo engafaki ikhodi enobude obude kuno-200 nt futhi aziwa ngokudlala indima ekuhleleni nasekulawuleni i-chromatin. Ubuchwepheshe bokulandelana komphumela ophezulu kanye ne-bioinformatic kunike amandla ukuqonda kwethu ukulandelana kwe-lncRNA nolwazi lokubeka endaweni ukuze kukhombe ama-lncRNA ngemisebenzi ebalulekile yokulawula. Leli payipi lihlinzeka ngokuhlaziywa kwe-lncRNA ngaphezu kokuhlaziya okushiwo epayipini Lokuhlaziya i-Eukaryotic mRNA.
16S/18S/ITS amplicon ukulandelana
Ipayipi lokuhlaziya i-Amplicon Sequencing microbial diversity lakhiwe ngokusekelwe eminyakeni yokuhlangenwe nakho kokuhlaziywa kwephrojekthi ye-microbial diversity. Ipayipi liqukethe ukuhlaziya okuyisisekelo okujwayelekile, okuhlanganisa okuqukethwe kokuhlaziya okujwayelekile kocwaningo lwamanje lwama-microbial kanye nokuhlaziya komuntu siqu. Umbiko wokuhlaziya unothile futhi ubanzi, unenketho yokwenza ukuhlaziya komuntu siqu okuhlukahlukene. Ukwengeza, amasampula namaqembu angashintshwa endizeni ukuze kwenziwe ngokwezifiso nokulawula okwengeziwe.
Shotgun Metagenomics Analysis
Ipayipi le-Shotgun Metagenomic Analysis lisebenzisa idatha ye-NGS evela ezintweni ezixubile ze-genomic ezikhishwe kumasampula emvelo. Ukuhlaziya okuhlanganisiwe kunikeza imininingwane enemininingwane ngokuhlukahluka kwezinhlobo nobuningi, ukwakheka kwabantu, ubudlelwano be-phylogenetic, izakhi zofuzo ezisebenzayo, kanye namanethiwekhi okuxhumana anezici zemvelo.
Ukuhlaziywa Okuhlukile kwe-NGS-WGS
Ukuhlaziywa Okuhlukile kwe-NGS-WGS ipayipi elihlanganisiwe lokuthola okuhlukile elenza ukulawulwa kwekhwalithi yedatha, ukuqondanisa kokulandelana, isichasiselo, nokuhlaziywa kokuguqulwa kofuzo. Ipayipi lilandela izinqubo ezihamba phambili ze-GATK zokutholwa kwe-SNP ne-InDel futhi isebenzisa i-Manta ekushayeni okuhlukile kwesakhiwo.
I-Genome Wide Association Study (GWAS)
Ipayipi le-GWAS liwukuhlaziywa komfula okuthatha amafayela e-VCF akhiqizwe ngaphambilini kanye nedatha ye-phenotype ehambisanayo yeqoqo labantu ngabanye njengokufaka. Isebenzisa izindlela ezithile zezibalo, i-GWAS ihlose ukwembula ukuhlukahluka kwe-nucleotide yohlobo olubanzi oluhlotshaniswa nomehluko we-phenotypic. Idlala indima ebalulekile ekuhloleni izakhi zofuzo ezisebenzayo ezihlobene nezifo eziyinkimbinkimbi zabantu kanye nezici eziyinkimbinkimbi ezitshalweni nasezilwaneni.
I-Bulk Segregation Analysis (BSA)
Ukuhlaziywa kwe-BSA kuhilela ukuhlanganisa abantu abanezimpawu ezeqisayo ze-phenotypic kusuka kubantu abahlukanisayo. Ngokuqhathanisa i-loci ehlukile phakathi kwamasampuli ahlanganisiwe, le ndlela ihlonza ngokushesha izimpawu zamangqamuzana ezihlotshaniswa nezakhi zofuzo eziqondiwe. Isetshenziswa kakhulu ekwenzeni imephu yofuzo yezitshalo nezilwane, iyithuluzi elibalulekile lokuzalanisa okusizwa umaka.
I-Evolutionary Genetics Analysis
I-Evolutionary Genetics Analysis ithuthukisa ulwazi olubanzi lwe-BMKGENE kumaphrojekthi wokuguquguquka kofuzo futhi ihlanganisa ukwakhiwa kwesihlahla se-phylogenetic, ukuhlaziywa kokungalingani, ukuhlola ukuhlukahluka kofuzo, ukuhlonza okukhethiwe, ukuhlaziya ukuhlobana, ukuhlaziywa kwengxenye eyinhloko, kanye nokuhlukaniswa kwesakhiwo sabantu.
