Tguide smart magnetic Plant RNA KINIT
Ikhathalogi Inombolo / Ukupakishwa
| Ikati. -nokuba | ID | Cha. Ama-preps |
| 4993552 (cartridge) | Dp662-de | 48 |
Umhlahlandlela wokukhethwa kwe-REEGENT ye-TGUIDE S16
| Nuclei acid | Uhlobo lwesampula | Igama lomkhiqizo | Usayizi wokupakisha (ama-preps) I-cartridge / ipuleti | Ikati cha. I-Cartridge / Plate |
| Uhlobo lwefananabi | Izicubu zezilwane | Tguide smart magnetic izicubu ze-DNA KIN | 48/96 | 4993547/4995038 |
| Uhlobo lwefananabi | Isitshalo nembewu | Tguide smart magnetic plant dna kit | 48 | 4993548 |
| Uhlobo lwefananabi | Inhlabathi / stool | Tguide smart inhlabathi / stool dna kit | 48 | 4993549 |
| Uhlobo lwefananabi | Imikhiqizo ye-GEL & PCR | TGUIDE Smart DNA HINISHINISAIT KIT | 48 | 4993550 |
| Uhlobo lwefananabi | Igazi lonke | Tguide smart igazi genom kit | 48/96 | 4993703/4995206 |
| Uhlobo lwefananabi | Iseli / i-swab / izindawo ezomile, njll | Tguide smart universal dna kit | 48/96 | 4993704/4995040 |
| Uhlobo lwanamvubelo | Igazi / Iseli / Izicubu | Tguide smart igazi / iseli / izicubu rna kit | 48/96 | 4993551/4995043 |
| Uhlobo lwanamvubelo | Isitshalo nembewu | Tguide smart magnetic Plant RNA KINIT | 48 | 4993552 |
| I-DNA / RNA | Amanzi wama-Cell Free (Serum, njll) | I-TGUITE SMART Viral DNA / RNA KIT | 48/96 | 4993702/4995207 |
Isingeniso Somkhiqizo
Hlanza isivuno esiphakeme, ubumsulwa obuphezulu, i-RNA ephezulu kakhulu ephelele egazini / iseli / izicubu.
Azikho ama-reagents anobuthi afana ne-phenol / chloroform equkethwe
I-RNA etholwe inobumsulwa obukhulu futhi ingasetshenziselwa ngqo ukutholwa kwe-chip, ukulandelana okuphezulu okuphezulu kanye nezinye izivivinyo.
Izici
Kulula ukuyisebenzisa
Awukho umsebenzi owengeziwe we-Pipetting.
IVolumu yokuqeda ingashintshwa njengoba kudingeka.
Ukufakwa nokusebenza kudinga ukuqeqeshwa okuncane. Ngezinhlelo ezifakwe kwangaphambili, khipha i-cartridge, khetha i-protocol bese uqhuba ukuhlolwa kwakho. Izinhlelo zilungele ukusetshenziswa, futhi ziyavumelana nezifiso.
Ama-reagents alayishwe kuqala kanye nokudla okulahlwa okulayiniwe.
Ukuphathwa kwamakhemikhali kuqedwa ngakude kakhulu ukuqinisekisa ukuphepha kokusebenza.
Umphumela wedemo
Isimo sempande kakolweni i-RNA
Isampula usayizi: 100 mg
Ukwenziwa kwesampula: ukugaya nge-nitrogen ye-liquid nitrogen noma ukwelashwa okuphansi kwe-homogenizer
I-Agarose Gel Concentration: 2% (TAE)
Iyalayisha ivolumu: 2 μl
UMarker: UMarker IV, iTiangen
Umphumela Wokuhlola: Kusetshenziswa i-TGUIDE S16 etholakele ngeReagent 4993552 ukukhipha ngokuzenzakalelayo i-RNA ephelele yezimpande zikakolweni zinesivuno esihle kanye nobumsulwa obukhulu. Kulesi sivivinyo, cishe ama-20 μg we-nucleic acid akhishwe kusuka ezimpandeni zikakolweni we-100 mg, nge-OD260 / OD280 azungeze 2.0 ~ 2.1, ne-OD260 / OD230> 2.0.
Isizinda se-RNA se-RNA esivela kumasampula ahlukene wesitshalo
Isampula usayizi: 100 mg
Isampula yokuhlehlisa: I-homogenizer ephansi
I-Agarose gel Concentration: 1% (TAE)
Iyalayisha ivolumu: 1 μl
M: UMarker III, TIANGEN
1-4: imbewu yommbila 5-8: ummbila ushiya 9-12: Izimpande zeklabishi 13-16: Soybeans
Amasampula amabili okuqala akhishwe yi-Spin-Colum-Colum Cutction Kit kanye namasampula amabili okugcina akhishwe nge-TGUIDE S16.
Umphumela Wokuhlola: Isivuno sokukhishwa kwesitshalo se-RNA nge-TGUIDE S16 nge-Reagent 4993552 ilingana noma ingcono kunaleyo ikhithi yokukhishwa kwekholomu yekholomu. Ubumsulwa nobuqotho empeleni bufana. Ngakho-ke, i-TGUIDE S16 ingasetshenziselwa ukukhipha isitshalo se-RNA ngokufakwa esikhundleni kwesixazululo sokukhishwa kwe-spin-colowm.
