I-TGuide Smart Blood/Cell/Tissue RNA Kit
Inombolo yekhathalogi / iphakheji
| Ikati. cha | ID | Inombolo ye-preps |
| 4995043(ipuleti) | DP661-E | 96 |
| 4993551(ikhatriji) | I-DP661-DE | 48 |
Umhlahlandlela wokukhetha i-reagent ye-TGuide S16
| I-Nuclei Acid | Uhlobo lwesampula | Igama Lomkhiqizo | Usayizi wokupakisha (preps) Ikhatriji/ ipuleti | Inombolo yekati. Ikhatriji/ipuleti |
| I-DNA | Izicubu Zezilwane | I-TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| I-DNA | Isitshalo Nembewu | I-TGuide Smart Magnetic Plant DNA Kit | 48 | 4993548 |
| I-DNA | Umhlabathi/Indle | TGuide Smart Soil /Stool DNA Kit | 48 | 4993549 |
| I-DNA | Imikhiqizo ye-Gel ne-PCR | TGuide Smart DNA Purification Kit | 48 | 4993550 |
| I-DNA | Igazi Eliphelele | I-TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| I-DNA | Iseli/I-Swab/Izindawo Ezomile, njll | I-TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| I-RNA | Igazi/Iseli/Izicubu | I-TGuide Smart Blood/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| I-RNA | Isitshalo Nembewu | I-TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| I-DNA/RNA | Uketshezi Lwamahhala Lweseli (iSerum, njll) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Isingeniso somkhiqizo
Hlanza isivuno esiphezulu, ukuhlanzeka okuphezulu, i-RNA yekhwalithi ephezulu egazini/iseli/izicubu.
Awekho ama-reagents anobuthi njenge-phenol/chloroform aqukethwe
I-RNA etholiwe inokuhlanzeka okuphezulu futhi ingasetshenziselwa ngokuqondile ukutholwa kwe-chip, ukulandelana kwe-high-throughput nokunye ukuhlola.
Izici
Kulula kakhulu ukusebenzisa
Awukho umsebenzi owengeziwe wamapayipi.
Ivolumu ye-elution ingalungiswa njengoba kudingeka.
Ukufakwa nokusebenza kudinga ukuqeqeshwa okuncane. Ngezinhlelo ezifakwe ngaphambilini, qaqa ikhathriji, khetha umthetho olandelwayo bese uqalisa ukuhlola kwakho. Izinhlelo sezilungele ukusetshenziswa, futhi zingenziwa ngendlela oyifisayo.
Ama-reagents alayishwe ngaphambili kanye nezinto ezisetshenziswayo ezilahlwayo.
Ukuphathwa kwamakhemikhali kuqedwa ngezinga elikhulu ukuze kuqinisekiswe ukuphepha kokusebenza.
Umphumela wedemo
I-Agilent 2100 yasetshenziselwa ukutholwa kobuqotho.
Umthombo oyisampula: CD-1 amagundane
Isimo sesampula: igazi elisha eliphelele
Usayizi wesampula: 200 μl
Umphumela wokuhlolwa: isamba se-RNA ekhishwe egazini yi-TGuide S16 ifakwe i-reagent 4993551 inesivuno esihle nokuhlanzeka okuphezulu, futhi ingahlangabezana nezidingo zomfula ongezansi.
ukulandelana komphumela ophezulu.
Umthombo oyisampula: CD-1 amagundane
Ivolumu yokulayisha: 1 μl. Isibindi sihlanjululwe izikhathi ezingu-5 futhi salayishwa.
Ukuhlushwa kwejeli ye-Agarose: 1%. I-Electrophoresis yemizuzu engu-20 ku-6 V / cm;
M:TIANGEN Umaka III 1-4: inhliziyo 5-8:
isibindi 9-12: iphaphu 13-16: izinso
Amasampuli amabili okuqala esithishu ngasinye akhishwe ikhithi yokukhipha esekelwe kukholomu espin, futhi amabili okugcina akhishwe ngokuzenzakalelayo yi-TGuide S16.
Umphumela wokuhlolwa: isamba sethishu yezilwane i-RNA ekhishwe ngokuzenzakalelayo yi-TGuide S16 ifakwe i-reagent 4993551 inomkhiqizo ophezulu nobumsulwa, okulingana ne-RNA ekhishwe yiphrothokholi yokukhipha esekelwe kukholomu. Ukuhlanzeka kuyafana ngokuyisisekelo, nge-OD260/OD280 cishe ku-2.0~2.1 kanye ne-OD260/OD230> 2.0. Sengiphetha, i-TGuide S16 ingasetshenziselwa ukukhipha isamba se-RNA ezicutshini zezilwane esikhundleni sesixazululo sokukhipha esisekelwe kukholomu.
