I-TGuide Smart Magnetic Plant DNA Kit
Inombolo yekhathalogi / iphakheji
| Ikati. cha | ID | Inombolo ye-preps |
| 4993548(ikhatriji) | DP607-DE | 48 |
Umhlahlandlela wokukhetha i-reagent ye-TGuide S16
| I-Nuclei Acid | Uhlobo lwesampula | Igama Lomkhiqizo | Usayizi wokupakisha (preps) Ikhatriji/ ipuleti | Inombolo yekati. Ikhatriji/ipuleti |
| I-DNA | Izicubu Zezilwane | I-TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| I-DNA | Isitshalo Nembewu | I-TGuide Smart Magnetic Plant DNA Kit | 48 | 4993548 |
| I-DNA | Umhlabathi/Indle | TGuide Smart Soil /Stool DNA Kit | 48 | 4993549 |
| I-DNA | Imikhiqizo ye-Gel ne-PCR | TGuide Smart DNA Purification Kit | 48 | 4993550 |
| I-DNA | Igazi Eliphelele | I-TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| I-DNA | Iseli/I-Swab/Izindawo Ezomile, njll | I-TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| I-RNA | Igazi/Iseli/Izicubu | I-TGuide Smart Blood/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| I-RNA | Isitshalo Nembewu | I-TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| I-DNA/RNA | Uketshezi Lwamahhala Lweseli (iSerum, njll) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Isingeniso somkhiqizo
Kusebenza kakhulu: Ilungele izinhlobonhlobo zezicubu zezitshalo, ikakhulukazi izitshalo ezicebile nge-polysaccharide- noma i-polyphenol.
Iphephile futhi ayinabo ubuthi: Awekho ama-organic reagents anobuthi njenge-phenol/chloroform aqukethwe.
Ukuhlanzeka okuphezulu: I-DNA etholiwe inokuhlanzeka okuphezulu futhi ingasetshenziselwa ngokuqondile ukutholwa kwe-chip, ukulandelana komphumela ophezulu nokunye ukuhlola.
Izici
Kulula kakhulu ukusebenzisa
Awukho umsebenzi owengeziwe wamapayipi.
Ivolumu ye-elution ingalungiswa njengoba kudingeka.
Ukufakwa nokusebenza kudinga ukuqeqeshwa okuncane. Ngezinhlelo ezifakwe ngaphambilini, qaqa ikhathriji, khetha umthetho olandelwayo bese uqalisa ukuhlola kwakho. Izinhlelo sezilungele ukusetshenziswa, futhi zingenziwa ngendlela oyifisayo.
Ama-reagents alayishwe ngaphambili kanye nezinto ezisetshenziswayo ezilahlwayo.
Ukuphathwa kwamakhemikhali kuqedwa ngezinga elikhulu ukuze kuqinisekiswe ukuphepha kokusebenza.
Umphumela wedemo
Ukukhishwa kwe-Genomic DNA yamazambane
Usayizi wesampula: 100 mg
Isampula yokwelashwa kwangaphambili: ukugaya nge-nitrogen ewuketshezi noma i-tishu homogenizer
Ijeli ye-Agarose :2% (TAE)
Ivolumu yokulayisha: 2 μl
Umaka: i-D15000, i-TIANGEN S16-01, i-S16-02 ne-S16-03 imele ukuhlola okufanayo kokukhipha i-DNA ye-genomic yezitshalo ngokusebenzisa i-3 TGuide S16 Nucleic Acid Extractors ngesikhathi esifanayo.
Inkampani: ibhrendi eyaziwayo
Umphumela wokuhlolwa: ukusebenzisa i-TGuide S16 ene-reagent 4993548 ukuze kukhishwe ngokuzenzakalelayo i-DNA ye-genomic yamazambane kuzuze isivuno esihle kanye nokuhlanzeka okuphezulu, okulingana nekhwalithi yomkhiqizo wokukhishwa kwamakhithi wokukhipha amakholomu aqhudelanayo aziwayo. Kulesi sivivinyo, cishe i-11 μg ye-nucleic acid yakhishwa ku-100 mg wamazambane efihlekile, ne-OD260/OD280 cishe ku-1.8 ~ 1.9, kanye ne-OD260/OD230＞2.0.
Ukukhishwa kwe-Genomic DNA yeqabunga likabhontshisi wesoya
Usayizi wesampula: 100 mg
Isampula yokwelashwa kwangaphambili: ukugaya nge-nitrogen ewuketshezi noma i-tishu homogenizer
Ijeli ye-Agarose :1% (TAE)
Ivolumu yokulayisha: 2 μl
Umaki: D15000, TIANGEN
Umphumela wokuhlola: ukusebenzisa i-TGuide S16 ene-reagent 4993548 ukuze kukhishwe ngokuzenzakalelayo i-DNA ye-genomic yamaqabunga kabhontshisi wesoya kuzuze isivuno esihle kanye nokuhlanzeka okuphezulu. Kulolu cwaningo, cishe i-13μg ye-nucleic acid ikhishwe kumaqabunga e-soya we-100 mg, ne-OD260 / OD280 ezungeze i-1.8 ~ 1.9, ne-OD260 / OD230＞2.0.
Ukukhishwa kwe-Genomic DNA yezimpande zikakolweni
Usayizi wesampula: 100 mg
Ukwelashwa kwangaphambili okuyisampula: ukugaya nge-nitrogen ewuketshezi noma izicubu zokugaya i-homogenizer
Ijeli ye-Agarose :1% (TAE)
Ivolumu yokulayisha: 2 μl
Umaki: D15000, TIANGEN
Inkampani: ibhrendi eyaziwayo
Umphumela wokuhlola: ukusebenzisa i-TGuide S16 ene-reagent 4993548 ukuze kukhishwe ngokuzenzakalelayo i-DNA ye-genomic yezimpande zikakolweni kuzuze isivuno esihle kanye nokuhlanzeka okuphezulu, okulingana nesivuno sokukhipha kanye nobumsulwa babancintisana nabo abaziwayo. Ngaphezu kwalokho, ubuqotho be-DNA ye-genomic bungcono kunemikhiqizo encintisanayo yezinye izinkampani. Kulesi sivivinyo, cishe i-15 μg ye-nucleic acid ikhishwe ezimpandeni zikakolweni ezingu-100 mg, ne-OD260 / OD280 ezungeze i-1.8 ~ 1.9, ne-OD260 / OD230＞2.0.
