Izifutho zokuziphendukela kwemvelo
Izinzuzo zensizakalo
Takagi et al.,Iphephabhuku lezitshalo, 2013
●Ukuhlaziywa okuphelele kwe-bioinformatic:ukunika amandla ukulinganisa kokuhlukahluka kofuzo, okubonisa amandla okuziphendukela kwemvelo ezinhlotsheni, futhi kuveze ubudlelwane obuthembekile be-phylogenetic phakathi kwezinhlobo ezinethonya elincishisiwe lokuziphendukela kwemvelo okufana nokufana kwemvelo
●Ukuhlaziywa okwenziwe ngokwezifiso: Njengokulinganisa kwesikhathi se-divergence kanye nesivinini ngokususelwa ekuhlukahlukani nasezingeni le-nucleotide nase-amino acid.
●Ubuchwepheshe obubanzi namarekhodi okushicilela: Bmkgene uqongelele isipiliyoni esikhulu kubantu kanye neminyaka engu-15, ehlanganisa izinkulungwane zezinhlobo, njll. Futhi kube nomthelela kumaphrojekthi angaphezu kwe-1000 aphezulu ashicilelwe ezokuxhumana kwemvelo, izitshalo zamangqamuzana, izitshalo ze-biotechnology Journal, njll.
● Iqembu elinamakhono amakhulu we-bioinformatics kanye nomjikelezo wokuhlaziya omfishane: Ngolwazi olukhulu ekuhlaziyweni kwe-genomics ethuthukisiwe, iqembu lika-bmkgene lihambisa ukuhlaziya okuphelele ngesikhathi sokuguqula okusheshayo.
● Ukwesekwa kwangemva kokuthengisa:Ukuzibophezela kwethu kunwebeka ngaphezu kokuphothulwa kwephrojekthi ngesikhashana sezinyanga ezi-3 ngemuva kokuthengisa. Ngalesi sikhathi, sinikezela ngokulandela iphrojekthi, usizo lokuxazulula inkinga, kanye ne-q & amaseshini ukubhekana nanoma yimiphi imibuzo ehlobene nemiphumela.
Ukucaciswa Kwensiza Nezidingo
| Uhlobo lokulandelana | Isilinganiso sabantu abanconyiwe | Ukuhlehlisa Isu | Izidingo ze-Nucleotide |
| Ukuhlelwa okulandelana kona konke | ≥ Abantu abangama-30, abanabantu abangu-10 abavela eqoqweni ngalinye
| Eliyo | Ukugxila: ≥ 1 ngl Inani eliphelele le-30ng Kukhawulelwe noma akukho ukucekelwa phansi noma ukungcoliswa |
| Isiqephu esikhethekile-se-Locus Amplified (SLAF) | Ukujula kwe-Tag: Eliyo Inani lamathegi: <400 MB: Kunconywe ama-WGS <1GB: Amathegi we-100k I-1GB > 2GB: Amathegi angama-300k Amathegi ama-max 500k | Ukugxila ≥ 5 ngl Inani eliphelele ≥ 80 ng I-Nanodrop OD260 / 280 = 1.6-2.5 I-Agarose Gel: Akukho ukuwohloka noma ukungcoliswa okulinganiselwe noma ukungcoliswa
|
Ukugeleza komsebenzi wesevisi
Idizayini yokuhlola
Ukulethwa kwesampula
Ukwakhiwa Kwelabhulali
Ukulandelana
Ukuhlaziywa kwedatha
Services After-Sale
Insizakalo ifaka phakathi ukuhlaziywa kwesakhiwo sabantu (i-phylogenetic Tree, i-PCA, i-Population Stratification Chart), ukwehluka kwesibalo sabantu, nokukhethwa kwesibalo (ukukhethwa kwe-counter aclequelivibrium, ukukhetha okukhethwayo okukhethiwe kwezindawo eziyinzuzo). Insizakalo nayo ingafaka ukuhlaziywa okwenziwe ngokwezifiso (isib. Isikhathi sokuphambukisa, ukugeleza kofuzo).
*Imiphumela yedemo eboniswe lapha konke kusuka kuma-genome ashicilelwe nge-BMKGENE
Ukuhlaziywa kwe-Evie Kuqukethe ukwakhiwa kwesihlahla se-phylogenetic, ukwakheka kwabantu kanye ne-PCA kususelwa ekuhlukanisweni kofuzo.
Umuthi we-phylogenetic umele ubudlelwano be-tatonomic kanye nokuziphendukela kwemvelo phakathi kwezinhlobo ezinokhokho ojwayelekile.
I-PCA ihlose ukubona ngeso lengqondo ukusondelana phakathi kwabantu abancane.
Isakhiwo sabantu sikhombisa ukuba khona kwesibalo sabantu abahlukile ngofuzo maqondana namafrikhthi we-allele.
Chen, et. al.,I-pnas, 2020
2.Khetha Ukushanela
Ukukhethwa okukhethiwe kubhekiswa kwinqubo lapho kukhethwe khona isiza esizuzisayo kanye namafrikhwensi ezindawo zokungathathi hlangothi ezixhunyiwe zandisiwe futhi lezo zezingosi ezingenampilo zehlisiwe, okuholela ekunciphiseni kwesifunda.
Ukutholwa okubanzi kwe-genome-okubanzi ngezindawo ezikhethiwe ezikhethiwe zicutshungulwa ngokubala i-Tountation genetic Index (π, FST, Tajima d) yazo zonke i-SNPs ngaphakathi kwewindows (100 KB) ngesinyathelo esithile (10 KB).
Ukwehluka kwe-nucleotide (π)
Tajima's d
Inkomba yokulungisa (FST)
Wu, et. al.,Isitshalo molecular, 2018
I-3.Gene Flow
Wu, et. al.,Isitshalo molecular, 2018
Umlando we-4.Demographic
Zhang, et. al.,Imvelo yemvelo nokuziphendukela kwemvelo, 2021
Isikhathi se-5.Divergence
Zhang, et. al.,Imvelo yemvelo nokuziphendukela kwemvelo, 2021
Hlola intuthuko eqhutshwa yizinsizakalo zofuzo ze-BMKGEne zokuziphendukela kwemvelo ngeqoqo elikhethiwe lokushicilelwa:
UHasnyar, AK et al. (2023) Ukutholwa kwezimpawu ze-snp molecular kanye nozakhitho zokhetho oluhlotshaniswa nokuphikiswa kwegciwane le-sacbrood ku-APIS Cerana Cerana izibungu ngokubuyiselwa kwe-genome genome ngokugcwele ',Ijenali Yezwe Yezizwe Yesayensi Ye-Molecular, 24 (7). DOI: 10.3390 / IJMS24076238.
I-Chai, J. et al. (2022) 'Ukutholwa kwe-Walamander yasendle emsulwa, yofuzo. Kwakha amathuba amasha okulondolozwa kwemvelo',Ucwaningo lwe-zoological, 2022, Vol. 43, 23, 3, amakhasi: 469-480, 43 (3), PP. 469-480. DOI: 10.24272 / J.issn.2095-8137.2022.101.
Han, M. et al. (2022) 'Iphethini ye-phylogeographical kanye nenani lokuziphendukela kwemvelo yendabuko u-Ilymus Sibiricus L. kuQinghai-Tibetan Plateau',Umngcele kusayensi yezitshalo, 13, k. 882601. Doi: 10.3389 / FPLS.202.882601 / Bibtex.
Wang, j. et al. .Ucwaningo lwe-Horticulture, 9. Doi: 10.1093 / HR / Uhac021.
