Imitapo yolwazi yangaphambi kwe-DNBSEQ
Izici
●Ipulatifomu:MGI-Dnbseq-T7
●Izindlela Zokulandelana:I-PE150
●Ukudluliswa kwemitapo yolwazi ye-Illina kuyoMGI:Inika amandla ukulandelana kokulandelana kwamanani aphezulu wedatha ngezindleko eziphansi.
●Ukulawulwa kwekhwalithi kwemitapo yolwazi ngaphambi kokulandelana.
●Ukuhlelwa kabusha kwedatha QC nokulethwa:Ukulethwa kombiko we-QC kanye nedatha eluhlaza ngefomethi ye-Fastq ngemuva kokudideka nokuhlunga kwe-Q30.
Izinzuzo
●Ukuguquguquka kwezinsizakalo zokulandelana:Ikhasimende lingase likhethe ukulandelana ngomzila noma inani ledatha.
●Ukukhishwa kwedatha ephezulu:I-1500 GB / Lane
●Ukulethwa kokulandelana kokulandelana kwe-QC:Ngama-metric asezingeni, ukunemba kwedatha nokusebenza okuphelele kwephrojekthi yokulandelana.
●Inqubo yokulandelana okuvuthiwe:ngokujika okufushane.
●Ukulawulwa kwekhwalithi okuqinile: Sisebenzisa izidingo eziqinile ze-QC ukuqinisekisa ukulethwa kwemiphumela esezingeni eliphakeme.
Izidingo zesampula
| Inani ledatha (x) | Ukuhlushwa (QPCCR / NM) | Ukuvimbanisa | |
| Umgwaqo ohlukile
| X ≤ 10 gb | ≥ 1nm | ≥ 25 μl |
| I-10 GB <X ≤ 50 GB | ≥ 2 nm | ≥ 25 μl | |
| I-50 gb <x ≤ 100 gb | ≥ 3 nm | ≥ 25 μl | |
| X> 100 gb | ≥ 4 nm | ||
| Umzila owodwa | Umzila ngamunye | ≥ 1.5 NM / I-Library Pool | I-≥ 25 μl / i-Library Pool |
Ngaphezu kwenani lokuhlushwa kanye nenani eliphelele, iphethini efanelekile ephakeme nayo iyadingeka.
QAPHELA: Ukulandelana kwemigwaqo yemitapo yolwazi ephansi yokuhlukahluka kudinga i-Phix Spike-in ukuqinisekisa ukubiza okuqinile kwesisekelo.
Sincoma ukuhambisa imitapo yolwazi efakwe kusengaphambili njengamasampula. Uma udinga i-BMKGENE ukwenza i-Pooling Pooling, sicela ubheke ku
Izidingo zomtapo wezincwadi zokulandelana kwale nkandlela.
Usayizi we-Library (Imephu Ye-Peak)
Inani elikhulu kufanele libe ngaphakathi kwe-300-450 BP.
Imitapo yolwazi kufanele ibe nesiqongo esiphezulu esiyinhloko, akukho ukungcoliswa kwe-adapter futhi akukho mibumba ye-primer.
Ukuhamba komsebenzi wesevisi
Umbiko weLabhulali QC
Umbiko ngekhwalithi yomtapo wezincwadi unikezwa ngaphambi kokulandelana, ukuhlola inani lomtapo wezincwadi, nokuqhekeka.
Ukuhlelwa kabusha kombiko we-QC
Ithebula 1. Izibalo ngokulandelana kwemininingwane.
| I-ID yesampula | IBMKID | Kufundwa okuluhlaza | Idatha eluhlaza (BP) | Ukufundwa okuhlanzekile (%) | Q20 (%) | Q30 (%) | I-GC (%) |
| C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | BMK_02 | 14,717,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Umdwebo 1. Ukusatshalaliswa kwekhwalithi okufundwayo okufundwayo kusampula ngayinye
Umdwebo 2. Isisekelo sokusatshalaliswa kokuqukethwe
Umdwebo 3. Ukusatshalaliswa kokuqukethwe okufundwayo ku-Sequecting Data
