I-Illumina eyenziwe imitapo yolwazi
Izici
●Amapulatifomu:I-Illumina Novaseq 6000 ne-Novaseq x Plus
●Izindlela Zokulandelana:Pe150 noPe250
●Ukulawulwa kwekhwalithi yemitapo yolwazi ngaphambi kokulandelana
●Ukuhlelwa kabusha kwedatha QC nokulethwa:Ukulethwa kombiko we-QC kanye nedatha eluhlaza ngefomethi ye-Fastq ngemuva kokudideka okudelela nokuhlunga kwe-Q30 Reads
Izinzuzo zensizakalo
●Ukuguquguquka kwezinsizakalo zokulandelana:Ikhasimende lingase likhethe ukulandelana ngomzila, i-Flow Cell, noma ngenani ledatha edingekayo (ukulandelwa kwemigwaqo okuyingxenye).
●Umuzwa obanzi endawenikazi yokulandelana kwe-Illinnanan:Njengoba izinkulungwane zamaphrojekthi avaliwe ngezinhlobo ezahlukahlukene.
●Ukulethwa kokulandelana kokulandelana kwe-QC:Ngama-metric asezingeni, ukunemba kwedatha nokusebenza okuphelele kwephrojekthi yokulandelana.
●Inqubo yokulandelana okuvuthiwe:ngokujika okufushane.
●Ukulawulwa kwekhwalithi okuqinile: Sisebenzisa izidingo eziqinile ze-QC ukuqinisekisa ukulethwa kwemiphumela esezingeni eliphakeme.
Amapulatifomu wesampula
| Indawo yokuqhudelana | Iseli yokugeleza | Imodi yokulandelana | Umuvo | Ukukhishwa okulinganiselwe |
| UNovaseq x | 10b (imizila engu-8) | I-PE150 | Umzila owodwa Umgwaqo ohlukile | 375GB / LAANE |
| 25b (8 imizila) | I-PE150 | Umzila owodwa Umgwaqo ohlukile | I-1000 GB / LAne | |
| UNovaseq 6000 | I-SP FLOGE Cell (2 LATES) | I-PE250 | Iseli yokugeleza Umzila owodwa Umgwaqo ohlukile | 325-400 m Ufunda / Umzila |
| Iseli le-S4 Flow (ama-4 lanes) | I-PE150 | Iseli yokugeleza Umzila owodwa Umgwaqo ohlukile | ~ 800 gb / lane |
Izidingo zesampula
| Inani ledatha (x) | Ukuhlushwa (QPCCR / NM) | Ukuvimbanisa | |
| Ukuhlelwa kabusha kwemigwaqo okuyingxenye
| X ≤ 10 gb | ≥ 1 nm | ≥ 25 μl |
| I-10 GB <X ≤ 50 GB | ≥ 2 nm | ≥ 25 μl | |
| I-50 gb <x ≤ 100 gb | ≥ 3 nm | ≥ 25 μl | |
| X> 100 gb | ≥ 4 nm | ≥ 25 μl | |
| Ukulandelana kwe-lane | Umzila ngamunye | ≥ 1.5 NM / I-Library Pool | I-≥ 25 μl / i-Library Pool |
Ngaphezu kwenani lokuhlushwa kanye nenani eliphelele, iphethini efanelekile ephakeme nayo iyadingeka.
QAPHELA: Ukulandelana kwemigwaqo yemitapo yolwazi ephansi yokuhlukahluka kudinga i-Phix Spike-in ukuqinisekisa ukubiza okuqinile kwesisekelo.
Sincoma ukuhambisa imitapo yolwazi efakwe kusengaphambili njengamasampula. Uma udinga i-BMKGENE ukwenza i-Poobling Pooling, sicela ubheke
izidingo zomtapo wezincwadi zokulandelana komgwaqo okuyingxenye.
Usayizi we-Library (Imephu Ye-Peak)
Inani elikhulu kufanele libe ngaphakathi kwe-300-450 BP.
Imitapo yolwazi kufanele ibe nesiqongo esiphezulu esiyinhloko, akukho ukungcoliswa kwe-adapter futhi akukho mibumba ye-primer.
Sicela usifinyelele kithi uma amasampula akho awahlangabezani nezidingo zokuqala ezibonakalayo.
Ukuhamba komsebenzi wesevisi
Ukulawulwa kwekhwalithi yeLabhulali
Ukulandelana
Isilawuli sekhwalithi yedatha
Ukulethwa Kwephrojekthi
Umbiko weLabhulali QC
Umbiko ngekhwalithi yomtapo wezincwadi unikezwa ngaphambi kokulandelana, ukuhlola inani lomtapo wezincwadi, nokuqhekeka.
Ukuhlelwa kabusha kombiko we-QC
Ithebula 1. Izibalo ngokulandelana kwemininingwane.
| I-ID yesampula | IBMKID | Kufundwa okuluhlaza | Idatha eluhlaza (BP) | Ukufundwa okuhlanzekile (%) | Q20 (%) | Q30 (%) | I-GC (%) |
| C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | BMK_02 | 14,717,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Umdwebo 1. Ukusatshalaliswa kwekhwalithi okufundwayo okufundwayo kusampula ngayinye
Umdwebo 2. Isisekelo sokusatshalaliswa kokuqukethwe
Umdwebo 3. Ukusatshalaliswa kokuqukethwe okufundwayo ku-Sequecting Data
