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条形 isibhengezo-03

Imikhiqizo

I-Chromatin Immunoprecipitation Sequencing (ChIP-seq)

I-Chromatin Immunoprecipitation (CHIP) iyindlela esebenzisa amasosha omzimba ukuze inothise ngokukhetha amaprotheni abopha i-DNA kanye nezinhloso zawo ezihambisanayo ze-genomics. Ukuhlanganiswa kwayo ne-NGS kunika amandla ukuprofetha okubanzi kofuzo lwezinjongo ze-DNA ezihlobene nokuguqulwa kwe-histone, izici zokuloba, namanye amaprotheni abopha i-DNA. Le ndlela eguquguqukayo inika amandla ukuqhathaniswa kwezindawo ezibophayo kuzo zonke izinhlobo zamaseli ahlukahlukene, izicubu, noma izimo. Izinhlelo zokusebenza ze-ChIP-Seq zisukela ekutadisheni imithetho yokubhala kanye nezindlela zokuthuthuka ziye ekucaciseni izindlela zezifo, okuyenza ibe ithuluzi elibalulekile lokuqonda ukubusa kokwakheka kwezwe kanye nokuthuthukiswa kwemininingwane yokwelapha.

Ipulatifomu: Illumina NovaSeq


Imininingwane Yesevisi

I-Bioinformatics

Imiphumela yedemo

Izinzuzo Zesevisi

Ukuhlaziya Okuthuthukisiwe Kwe-Bioinformatic kanye Nesichasiselo Esibanzi:Sisebenzisa imininingo egciniwe eminingi ukuze sichaze ngokusebenza izakhi zofuzo ezihambisana nezifunda zokubophezela amaprotheni-DNA, sinikeze imininingwane ngezinqubo zamangqamuzana namangqamuzana angaphansi kokusebenzelana.

Ukusekelwa Kwangemva Kokuthengisa:Ukuzinikela kwethu kudlulela ngalé kokuphothulwa kwephrojekthi ngenkathi yesevisi yezinyanga ezi-3 ngemva kokuthengisa. Ngalesi sikhathi, sinikezela ngokulandelelwa kwephrojekthi, usizo lokuxazulula inkinga, namaseshini e-Q&A ukuze siphendule noma yimiphi imibuzo ehlobene nemiphumela.

Umuzwa obanzi:Ngomlando wokuqeda ngempumelelo amaphrojekthi amaningi e-ChIP-Seq, inkampani yethu iletha ubuchwepheshe obungaphezu kweshumi leminyaka etafuleni. Ithimba lethu lokuhlaziya elinekhono eliphezulu, elihambisana nokuqukethwe okuphelele nokusekelwa kwangemva kokuthengisa, liqinisekisa impumelelo yamaphrojekthi akho.

● Ukulawulwa Kwekhwalithi Okuqinile: Sisebenzisa amaphuzu okulawula abalulekile kuzo zonke izigaba, kusukela kusampula nokulungiselela umtapo wezincwadi ukuya ekulandeleni nasekulandeleni i-bioinformatics. Lokhu kuqapha ngokucophelela kuqinisekisa ukulethwa kwemiphumela ehlala njalo yekhwalithi ephezulu.

Imininingwane Yesevisi

Umtapowolwazi

Isu Lokulandelanisa

Okuphumayo kwedatha okunconyiwe

Ikhwalithi yokulawula

I-DNA ehlanzekile ngemuva kwe-Immunoprecipitation

I-Ilumina PE150

I-10Gb

Q30≥85%

Ukuguqulwa kwe-Bisulfite >99%

Ukusebenza kahle kokusika kwe-MspI > 95%

Izidingo Zesampula

Inani eliphelele: ≥10 ng

Ukusatshalaliswa kosayizi wesiqephu: 100-750 bps

Ukugeleza Komsebenzi Wesevisi

ukulethwa kwesampula

Ukulethwa kwesampula

Ukulungiselela Umtapowolwazi

Ukwakhiwa komtapo wolwazi

Ukulandelanisa

Ukulandelanisa

Ukuhlaziywa kwedatha

Ukuhlaziywa kwedatha

Ngemva kokudayiswa Services

Amasevisi angemuva kokuthengisa


  • Okwedlule:
  • Olandelayo:

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    Kufaka ukuhlaziya okulandelayo:

    ● Ukulawulwa kwekhwalithi yedatha eluhlaza

    ● Ukushaya okuphezulu okusekelwe ekwenziweni kwemephu kuya ku-genome

    ● Isichasiselo sezakhi zofuzo ezihambisana nenani eliphakeme

    ● Ukuhlaziywa kwe-Motif: ukuhlonza amasayithi abophezelayo e-transcription factor (TFBS)

    ● Ukuhlaziya Okuphakeme Okuhlukile nesichasiselo

    Ukuhlolwa kokunothisa okuseduze ne-Transcription Starting Sites (TSSs)

     

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    Ukusatshalaliswa okubanzi kwe-genome kweziqongo ze-CHIP

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    Ukuhlukaniswa kwezifunda eziphakeme kakhulu

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    Ukucebisa okusebenzayo kwezakhi zofuzo ezihlobene nenani eliphakeme (KEGG)

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    thola isilinganiso

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