I-16S / 18S / I-Amplicon Sequencing-Pacbio
Izici Zensizakalo
● Indawo yokulandelana: PACBIO Revuo
● Imodi yokulandelana: Ama-CCS (I-HIFI Iyafunda)
● Ukukhuliswa kwesifunda esiqondiwe kulandelwa ukuxhumeka kwe-tandem kwama-amplicons ngaphambi kokulungiselela i-hifi smrt bell Library
Izinzuzo zensizakalo
●Isinqumo esiphakeme se-tatonomic: than short-amplicon ukulandelana,Inika amandla amanani aphezulu wokuhlukaniswa kwe-Otu ezingeni lezinhlobo.
●Ukubiza okunembe kakhulu isisekelo: Ukulandelana kwemodi ye-PACBIO CCS (Ukufundwa kwe-HIFI).
●I-Solarte-Free: Ukukhonjwa okusheshayo kokuqanjwa kwe-microbial kumasampula wezemvelo.
●Kusebenza Kakhulu: Izifundo ezahlukahlukene zomphakathi we-microbial.
●Ukuhlaziywa okuphelele kwe-bioinformatic: Iphakheji yakamuva ye-Qiime2 (ukuqonda okuphezulu kwemvelo yemvelo ye-microbial) enobuhlakani obuhlukahlukene ngokuya ngemininingwane yedatha, isichasiselo, i-OTU / ASV.
●Ubuchwepheshe obubanzi: Ngezinkulungwane zamaphrojekthi wokulandelana we-Amplicon owenziwe minyaka yonke, iBmkgene iletha ngaphezulu kweminyaka eyishumi yokuhlangenwe nakho, iqembu lokuhlaziya elinamakhono kakhulu, okuqukethwe okuphelele, nokusekelwa okuhle kakhulu kokuthengisa.
Ukucaciswa Kwensiza
| Indlu ehlala izincwadi | Ukuhlehlisa Isu | Kunconywe idatha |
| Umbala we-Amplicon | I-Pacbio Revuo | 10/30/50 k tags (CCS) |
Izidingo zesampula
| Ukuhlushwa (ng / μl) | Inani eliphelele (μg) | Ivolumu (μL) |
| ≥5 | ≥0.3 | ≥20 |
Kunconywe ukulethwa kwezinkampu
Frizela amasampula ku-nitrogen ewuketshezi amahora ama-3-4 bese ugcina ku-nitrogen noma -80 degree ekubekelweni isikhathi eside. Ukuhanjiswa kwesampula nge-ice eyomile kuyadingeka.
Ukugeleza komsebenzi wesevisi
Ukulethwa kwesampula
Ukwakhiwa Kwelabhulali
Ukulandelana
Ukuhlaziywa kwedatha
Services After-Sale
Kubandakanya ukuhlaziywa okulandelayo:
● Ukulawulwa kwekhwalithi yedatha eluhlaza
● I-OTU Clustering / de-Noise (ASV)
● Isichasiselo se-OTU
● Ukuhlaziywa kokuhlukahluka kwe-alpha: izinkomba eziningi, kufaka phakathi iShannon, Simpson no-Ace.
● Ukuhlaziywa kokuhlukahluka kwe-beta
● Ukuhlaziywa kweqembu lokuhlangana
● Ukuhlaziywa kokuxhumeka: phakathi kwezinto zezemvelo kanye nokwakheka kanye nokwehlukahlukana
● Ukubikezela kohlobo lwezinhlobo eziyi-16s
I-histogram yokusatshalaliswa kwetekisi
Isihlahla Sokusatshalaliswa Komphakathi Phylogenetic Tree
Ukuhlaziywa kwe-Alpha ukwehluka: ACE
into ekhombayo
Ukuhlaziywa kokuhlukahluka kwe-beta: PCOA
Ukuhlaziywa kweqembu: ANOVA
Hlola intuthuko elungiswe izinsizakalo zokulandelana kwe-BMKGENE ze-Amplicon ngePacbio ngeqoqo elikhethiwe lokushicilelwa.
UGao, X. kanye neWang, H. (2023) 'Ukuhlaziywa kuka-2023) kwamaphrofayili we-rumen ama-bacterial nemisebenzi ngesikhathi sokuhlekisa nge-adnology ku-alpine merino izimvu ezinezitebhisi ezimbili ezikhulayo', ukuvutshelwa, 9 ( 1), k. 16. I-Doi: 10.3390 / Fermentation9010016 / S1.
I-LI, S. et al. (2023) 'Ukuthwebula izinto ezimnyama ze-microbial emhlabathini wasogwadule usebenzisa ama-metagenomics asuselwa kwisiko kanye nokuhlaziywa okuphezulu' Doi: 10.1038 / S41522-023-00439-8.
Mu, l. Et al. . 1487. DOI: 10.1002 / JSFA.11482.
U-Yang, J. et al. . E-Oxazolone-kufakwe amathumbu amathumbu oxidative stress ku-antiobrafish ', ama-antioxidants 2023, vol. 12, ikhasi 192, 12 (1), k. 192. I-DoI: 10.3390 / Antiox12010192.
