Nanopore ni kikun

Awọn ọna ti o lagbara fun Nanopome jẹ ọna ti o lagbara fun awọn CDNAS ipari kikun, ti idanimọ ati ipilẹ awọn ilana lilo ilana jẹ ilana. The BMKCloud Nanopore Full-Length Transcriptome Pipeline is designed to analyze RNA-Seq data generated on the Nanopore platform against a high-quality well-annotated reference genome, providing qualitative and quantitative analysis at both the gene and transcript level. Lẹhin iṣakoso didara, aisipo gigun ni kikun (FLNC) ni a gba ati awọn ile-iṣẹ ṣiṣe deede ti wa ni aworan si Genome itọkasi lati yọ awọn iwe afọwọkọ yiyọ kuro. Lati ṣeto gbigbe, ikosile jẹ tito si ati iyatọ si awọn Jiini ati awọn iwe afọwọkọ ni a damo ati iṣẹ imurasilẹ. Pepoline tun pẹlu poyadenty yiyan (Apa), itupalẹ pipin yiyan, asọtẹlẹ ti o baamu, itupalẹ Transtor, asọtẹlẹ ti o tẹẹrẹ, asọtẹlẹ ti awọn ẹbun aramada ati ilana ilana ti awọn iwe afọwọkọ.