De Novo Engelome Apejọ
BMKGEE nfunni awọn solusan wapọ fun awọn ifunfun pẹlu olupọn, mimu ounjẹ si awọn aini iwadii oniruuru ati pipe jinlẹ julọ. Lilo-kukuru-ka ina mọnamọna kukuru ti a nikan gba iran ti Ereny Ilu kan. Awọn kika kukuru ati iṣeeṣe ipari lilo nanopore tabi pacbio ni a papọ fun Geneome ti o sọ diẹ sii pẹlu awọn iyika gigun. Pẹlupẹlu, ṣipọ Hi-C ti n ṣiṣẹ siwaju si imudarasi awọn agbara, gbigba iyọrisi ti ẹla iyenye patapata.
Awọn ẹya iṣẹ
Pẹlu awọn aṣayan o ṣeeṣe mẹta lati yan lati da lori iwọn ti o fẹ pipadanu jiini:
Ohun yiyan Rounme: Kukuru-Kawe-Kukuru pẹlu Ijiko Novaseq pe150.
● FUNGEL TUGE VEREOME:
Iwadi Aago: Ellia Nonaseq pe150.
Apejọ Gúyè: Pacbio Revio (hifi ka) tabi olokiki protore 48.
● Konomel ipele-ipele:
Iwadi Aago: Ellia Nonaseq pe150.
Apejọ Gúyè: Pacbio Revio (hifi ka) tabi olokiki protore 48.
Cnig Damoring pẹlu Apejọ Hi-C.
Awọn anfani Iṣẹ
●Ọpọ awọn ilana iṣedede ti o wa: Fun awọn ibi-iṣẹ iwadi oriṣiriṣi ati awọn ibeere ti pipadanu jiini
●Pipin iṣẹ iṣẹ Bioinformation:Eyi pẹlu Apejọ Genehome ati asọtẹlẹ ti awọn eroja jiini pupọ, Gene anfani Genetotion, ati Sisọ kadopin.
●Imọ-jinlẹ lọpọlọpọ: Pẹlu awọn ọrun-ilẹ Moobelia ti o pejọ, a mu ẹgbẹ kan ti iriri pọ si, ẹgbẹ onínọmbà ti o ni oye pupọ, nipa akoonu tita ọja, ati atilẹyin tita ọja.
●Atilẹyin titale:Iresi wa loke ipari iṣẹ-ṣiṣe pẹlu akoko iṣẹ iṣowo 3 lẹhin-oṣu. Lakoko yii, a pese atẹle iṣẹ akanṣe iṣẹ akanṣe, Iranlọwọ Iranlọwọ, ati awọn akoko Q & A lati koju awọn ibeere eyikeyi ti o ni ibatan si awọn abajade.
Awọn alaye iṣẹ
| Iṣẹ | Nwon nwon.Mirza | Iṣakoso Didara |
| Draft liame | Illuna Pe150 100X | Q30≥85% |
| Itanran eyeome | Iwadi Aago: Orilina Pe150 50 x Apejọ: Pacbio hifi 30x tabi nanopore 100x | Didit N50 ≥1MB (Pacbio Aikọ) Didit N50 ≥2MB (Oníṣe) Didig N50 ≥500kb (awọn miiran) |
| Chromonome ipele ti ipele | Iwadi Aago: Orilina Pe150 50 x Apejọ: Pacbio hifi 30x tabi nanopore 100x Hi-c apejọ 100x | Clig Clamorin (90%
|
Awọn ibeere Iṣẹ
| Fojusi (ng / μl) | Lapapọ iye (μg) | Iwọn didun (μl) | Od260 / 280 | OD260 / 230 | |
| Pacbio | ≥20 | ≥2 | ≥20 | 1.7-2.2 | ≥1.6 |
| Nanotore | ≥40 | ≥2 | ≥20 | 1.7-2.2 | 1.0-3.0 |
| Ina mọnamọna | ≥1 | ≥0.06 | ≥20 | - | - |
Fungus alailẹgbẹ: ≥3.5x1010 awọn sẹẹli
Makiro fungus: ≥10 g
Iṣẹ Iṣẹ Iṣẹ sisan
Ifijiṣẹ apẹẹrẹ
Ile-ikawe Ile-ikawe
Iṣẹ ṣiṣe
Itupalẹ data
Awọn iṣẹ lẹhin-ẹrọ
Pẹlu itupalẹ ti o tẹle:
Iwadi Aago:
- Iṣakoso didara data
- Iwaju Genome: Iwọn, Hetezytority, awọn eroja atunwi
Apejọ Genome itanran:
- Iṣakoso didara data
- DenoApejọ
- Onínọmbà paati: asọtẹlẹ ti CDS ati awọn eroja lientic pupọ
- Alaye ṣiṣe pẹlu awọn apoti isura data gbogbogbo (lọ, keke, bbl) ati awọn apoti isura ibi ipamọ (kaadi
Apejọ Hi-C:
- Ibalopo Ibalopo Hi-C.
- Clits Clashing clustering, paṣẹ ati iṣalaye
- Igbelewọn ti apejọ hi-c: da lori itumọ ọrọ ati maapu igbona
Iwadi Aago: Pinpin K-MER
Apejọ Genes: Alaye ti Howelolomote (data data)
Apejọ Genen: Genenation Genenatation (Lọ)
Ṣawari awọn ilọsiwaju ti o ni irọrun nipasẹ awọn iṣẹ apejọ BMKGEN ti BMKGEL nipasẹ gbigba ti awọn atẹjade.
Hao, J. et al. (2023) 'isopọ Omotic Omac ti o jẹ ti oluso ti oogun ti o ni oogun ti awọn ipo ti o ni agbara,BMC Genomics, 24 (1), PP. 1-12. Ise: 10,1186 / S12864-023-0266-Z / Awọn iṣiro / 3.
Lu, L. et al. (2023) 'gquomecation gquomecation ṣafihan itankalẹ ati awọn imu-ara pathogenic ti alikat Pathort Patsegen Rèben Rhizonsia,Awọn akosile na, 11 (2), PP. 405-416. Ise: 10,1016 / JOCJ.202.07.024.
Zhang, H. et al. (2023) 'Awọn orisun Gallome' '' Gallome fun awọn eya mẹrin claririedia nfa iranran dola lori awọn turfgrasses Oniruuru ',Arun ọgbin, 107 (3), PP. 929-934. Ise: 10,1094 / PDIS-08-22-1921 - a
Zhang, SS et al. (2023) 'ẹda jiini ati ohun mimu ti o ni agbara ti ẹya ti o jẹ teshela frondosa',Iwe akosile ti elu, 9 (10), p. 959. Ise: 10.33390 / JOF9100959 / S1.
