TGuide Smart oofa Plant RNA Kit
Nọmba katalogi / apoti
| Ologbo. rara | ID | No. ti preps |
| 4993552(katiriji) | DP662-DE | 48 |
Itọsọna yiyan Reagent fun TGuide S16
| Ekuro Acid | Apeere Iru | Orukọ ọja | Iwọn iṣakojọpọ (awọn igbaradi) Katiriji/ awo | Ologbo No. Katiriji / awo |
| DNA | Ẹranko Tissue | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Ohun ọgbin & Irugbin | TGuide Smart oofa Plant DNA Kit | 48 | 4993548 |
| DNA | Ile/Otita | TGuide Smart Ile / Otita DNA Kit | 48 | 4993549 |
| DNA | Jeli & PCR awọn ọja | TGuide Smart DNA ìwẹnumọ Apo | 48 | 4993550 |
| DNA | Gbogbo Ẹjẹ | TGuide Smart Ẹjẹ Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Ẹyin/Swab/Awọn aaye gbigbẹ, ati bẹbẹ lọ | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Ẹjẹ / Ẹjẹ / Tissue | TGuide Smart Ẹjẹ / Ẹjẹ / Tissue RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Ohun ọgbin & Irugbin | TGuide Smart oofa Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | Awọn Omi Ọfẹ sẹẹli (Omi-ara, ati bẹbẹ lọ) | TGuide Smart Gbogun ti DNA/RNA Kit | 48/96 | 4993702/4995207 |
ifihan ọja
Sọ eso-giga, mimọ-giga, RNA lapapọ ti o ni agbara giga lati ẹjẹ/sẹẹli/ara.
Ko si awọn reagents majele bii phenol/chloroform ninu
RNA ti o gba ni mimọ ti o ga ati pe o le ṣee lo taara fun wiwa chirún, ilana ṣiṣe-giga ati awọn adanwo miiran.
Awọn ẹya ara ẹrọ
Super rọrun lati lo
Ko si afikun iṣẹ pipetting.
Iwọn didun elution le ṣe atunṣe bi o ṣe nilo.
Fifi sori ẹrọ ati iṣiṣẹ nilo ikẹkọ kekere. Pẹlu awọn eto ti a ti fi sii tẹlẹ, ṣii katiriji naa, yan ilana naa ati ṣiṣe idanwo rẹ. Awọn eto ti ṣetan-lati-lo, ati tun ṣe isọdi.
Awọn atunto ti kojọpọ tẹlẹ ati awọn ohun elo isọnu isọnu ti o baamu.
Imudani kemikali jẹ imukuro si iye ti o tobi julọ lati rii daju aabo iṣẹ.
Abajade Ririnkiri
Alukama root lapapọ isediwon RNA
Iwọn apẹẹrẹ: 100 mg
Itọju iṣapeye: lilọ pẹlu nitrogen olomi tabi itọju iṣọn-ara ti iwọn otutu kekere
Idojukọ gel Agarose: 2% (TAE)
Iwọn ikojọpọ: 2 μl
Ami: Alami IV, TIANGEN
Abajade idanwo: lilo TGuide S16 ti o ṣaṣeyọri pẹlu reagent 4993552 lati yọkuro RNA lapapọ ti awọn gbongbo alikama ni ikore to dara ati mimọ giga. Ninu idanwo yii, nipa 20 μg ti acid nucleic ni a fa jade lati awọn gbongbo alikama miligiramu 100, pẹlu OD260/OD280 ni ayika 2.0 ~ 2.1, ati OD260/OD230＞2.0.
Lapapọ isediwon RNA lati oriṣiriṣi awọn ayẹwo ọgbin
Iwọn apẹẹrẹ: 100 mg
Apeere pretreatment: kekere-otutu homogenizer
Idojukọ gel Agarose: 1% (TAE)
Iwọn ikojọpọ: 1 μl
M: Alami III, TIANGEN
1-4: irugbin agbado 5-8: ewe oka 9-12: gbongbo eso kabeeji 13-16: soybeans
Awọn ayẹwo meji akọkọ ni a fa jade nipasẹ ohun elo isediwon-iwe ati awọn ayẹwo meji ti o kẹhin ni a fa jade nipasẹ TGuide S16.
Abajade idanwo: ikore isediwon ti ọgbin RNA nipasẹ TGuide S16 pẹlu reagent 4993552 jẹ deede si tabi dara julọ ju iyẹn lọ nipasẹ ohun elo isediwon-iwe. Awọn ti nw ati iyege ni o wa besikale awọn kanna. Nitorinaa, TGuide S16 le ṣee lo lati jade lapapọ RNA ọgbin ni rirọpo ojutu isediwon orisun-alapin.
