TGuide Smart Ẹjẹ / Ẹjẹ / Tissue RNA Kit
Nọmba katalogi / apoti
| Ologbo. rara | ID | No. ti preps |
| 4995043(awo) | DP661-E | 96 |
| 4993551(katiriji) | DP661-DE | 48 |
Itọsọna yiyan Reagent fun TGuide S16
| Ekuro Acid | Apeere Iru | Orukọ ọja | Iwọn iṣakojọpọ (awọn igbaradi) Katiriji/ awo | Ologbo No. Katiriji / awo |
| DNA | Ẹranko Tissue | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Ohun ọgbin & Irugbin | TGuide Smart oofa Plant DNA Kit | 48 | 4993548 |
| DNA | Ile/Otita | TGuide Smart Ile / Otita DNA Kit | 48 | 4993549 |
| DNA | Jeli & PCR awọn ọja | TGuide Smart DNA ìwẹnumọ Apo | 48 | 4993550 |
| DNA | Gbogbo Ẹjẹ | TGuide Smart Ẹjẹ Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Ẹyin/Swab/Awọn aaye gbigbẹ, ati bẹbẹ lọ | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Ẹjẹ / Ẹjẹ / Tissue | TGuide Smart Ẹjẹ / Ẹjẹ / Tissue RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Ohun ọgbin & Irugbin | TGuide Smart oofa Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | Awọn Omi Ọfẹ sẹẹli (Omi-ara, ati bẹbẹ lọ) | TGuide Smart Gbogun ti DNA/RNA Kit | 48/96 | 4993702/4995207 |
ifihan ọja
Sọ eso-giga, mimọ-giga, RNA lapapọ ti o ni agbara giga lati ẹjẹ/sẹẹli/ara.
Ko si awọn reagents majele bii phenol/chloroform ninu
RNA ti o gba ni mimọ ti o ga ati pe o le ṣee lo taara fun wiwa chirún, ilana ṣiṣe-giga ati awọn adanwo miiran.
Awọn ẹya ara ẹrọ
Super rọrun lati lo
Ko si afikun iṣẹ pipetting.
Iwọn didun elution le ṣe atunṣe bi o ṣe nilo.
Fifi sori ẹrọ ati iṣiṣẹ nilo ikẹkọ kekere. Pẹlu awọn eto ti a ti fi sii tẹlẹ, ṣii katiriji naa, yan ilana naa ati ṣiṣe idanwo rẹ. Awọn eto ti ṣetan-lati-lo, ati tun ṣe isọdi.
Awọn atunto ti kojọpọ tẹlẹ ati awọn ohun elo isọnu isọnu ti o baamu.
Imudani kemikali jẹ imukuro si iye ti o tobi julọ lati rii daju aabo iṣẹ.
Abajade Ririnkiri
Agilent 2100 ni a lo fun wiwa iduroṣinṣin.
Apeere orisun: CD-1 eku
Ipo apẹẹrẹ: gbogbo ẹjẹ titun
Iwọn apẹẹrẹ: 200 μl
Abajade idanwo: lapapọ RNA ti o jade lati inu ẹjẹ nipasẹ TGuide S16 ti o ni ipese pẹlu reagent 4993551 ni eso ti o dara ati mimọ giga, ati pe o le pade awọn iwulo ti isalẹ
ga-nipasẹ ọkọọkan.
Apeere orisun: CD-1 eku
Iwọn ikojọpọ: 1 μl. Ẹdọ ti fomi ni igba 5 ati ki o kojọpọ.
Idojukọ gel Agarose: 1%. Electrophoresis fun 20 min ni 6 V / cm;
M: TIANGEN Marker III 1-4: ọkàn 5-8:
ẹdọ 9-12: ẹdọfóró 13-16: kíndìnrín
Awọn ayẹwo meji akọkọ ti ara kọọkan ni a fa jade nipasẹ ohun elo isediwon orisun-iwe, ati awọn meji ti o kẹhin ni a fa jade laifọwọyi nipasẹ TGuide S16.
Abajade idanwo: lapapọ ẹran ara RNA ti o yọ jade laifọwọyi nipasẹ TGuide S16 ti o ni ipese pẹlu reagent 4993551 ni ikore giga ati mimọ, eyiti o jẹ deede si RNA ti a fa jade nipasẹ ilana isediwon orisun-iwe. Iwa mimọ jẹ ipilẹ kanna, pẹlu OD260 / OD280 ni ayika 2.0 ~ 2.1 ati OD260 / OD230> 2.0. Ni ipari, TGuide S16 le ṣee lo lati jade lapapọ RNA lati awọn ẹran ara ẹranko ni rirọpo ojutu isediwon orisun-alapin.
