De novo Bakteria Genome Apejọ
A pese iṣẹ apejọ genome pipe ti kokoro arun, ti o ni idaniloju awọn ela 0. Eyi ṣee ṣe nipa sisọpọ awọn imọ-ẹrọ itẹlera kika gigun, gẹgẹbi Nanopore ati PacBio fun apejọ ati kika kukuru kukuru pẹlu Illumina fun afọwọsi apejọ ati atunṣe aṣiṣe ti awọn kika ONT. Iṣẹ wa n pese iṣan-iṣẹ bioinformatic pipe lati apejọ, asọye iṣẹ, ati itupalẹ bioinformatic ti ilọsiwaju, mimu awọn ibi-afẹde iwadii kan pato ṣẹ. Iṣẹ yii ngbanilaaye idagbasoke awọn genomes itọkasi deede fun ọpọlọpọ awọn ẹkọ-jiini ati jiini. Ni afikun, o jẹ ipilẹ fun awọn ohun elo bii iṣapeye igara, imọ-ẹrọ jiini, ati idagbasoke imọ-ẹrọ makirobia, ni idaniloju igbẹkẹle ati data jiini-ọfẹ aafo pataki fun ilọsiwaju awọn oye imọ-jinlẹ ati imọ-ẹrọ imọ-ẹrọ.
Awọn ẹya ara ẹrọ Iṣẹ
● Pẹlu awọn aṣayan meji ti o ṣee ṣe lati yan lati da lori iwọn ti o fẹ ti aṣepe genome.
● Aṣayan Jiini Akọpamọ: Atẹle kika kukuru pẹlu Illumina NovaSeq PE150.
● Pari 0 aafo genome.
● Ilana kika gigun lori Nanopore PromethION 48 tabi PacBio Revio fun apejọ genome.
● Atẹle kika kukuru lori Illumina NovaSeq fun afọwọsi genome ati atunse aṣiṣe (Nanopore) tabi lati ṣe agbekalẹ ẹda-ara kan.
Awọn anfani Iṣẹ
●Odo-Gap Genome Ẹri: Eyi jẹ nitori iṣọpọ ti ilana Illumina pẹlu kika kika gigun (Nanopore tabi PacBio).
●Ise sise Bioinformatics pipe:Eyi pẹlu apejọ jiini ati asọtẹlẹ ti awọn eroja genomic pupọ, asọye apilẹṣẹ iṣẹ ṣiṣe, ati awọn iwoye genome gẹgẹbi Idite Circos.
●Amoye nla: Pẹlu awọn genomes microbial ti o ju 20,000 ti o pejọ, BMKGENE mu ni iriri ọdun mẹwa kan, ẹgbẹ itupalẹ oye ti o ga julọ, akoonu okeerẹ, ati atilẹyin awọn tita-lẹhin to dara julọ.
●Atilẹyin Tita-lẹhin:Ifaramo wa kọja ipari iṣẹ akanṣe pẹlu oṣu mẹta lẹhin-tita akoko iṣẹ. Lakoko yii, a funni ni atẹle iṣẹ akanṣe, iranlọwọ laasigbotitusita, ati awọn akoko Q&A lati koju eyikeyi awọn ibeere ti o ni ibatan si awọn abajade.
●Ọ̀pọ̀lọpọ̀ Ọ̀nà Ìtẹ̀síwájú Tó Wà:Fun awọn ibi-afẹde iwadii oriṣiriṣi ati awọn ibeere ti aṣepari genome.
Awọn pato Iṣẹ
| Iṣẹ | Ilana titele |
| Akọpamọ Genome | Illumina PE150 100x |
| 0 Aafo Genome | Nanopore 100x + Illumina PE150 100x Or Pacbio HiFi 30x + Illumina PE150 100x (aṣayan) |
Awọn ibeere apẹẹrẹ:
| Ifojusi (ng/µL) | Apapọ iye (µg) | Iwọn (µL) | OD260/280 | OD260/230 | |
| PacBio | ≥20 | ≥1.2 | ≥20 | 1.7-2.2 | ≥1.0 |
| Nanopore | ≥40 | ≥2 | ≥20 | 1.7-2.2 | 1.0-3.0 |
| Illumina | ≥1 | ≥0.06 | ≥20 | - | - |
· Awọn kokoro arun: ≥3.5x1010 awọn sẹẹli
Sisan Iṣẹ Iṣẹ
Ifijiṣẹ apẹẹrẹ
Ikole ìkàwé
Titele
Itupalẹ data
Lẹhin-tita awọn iṣẹ
Pẹlu Itupalẹ atẹle yii:
● Sequencing Data Didara Iṣakoso
● Apejọ Genome
● Itupalẹ Ẹka Genom: Asọtẹlẹ ti CDS ati awọn eroja genomic pupọ
● Apejuwe iṣẹ-ṣiṣe pẹlu ọpọlọpọ awọn apoti isura infomesonu gbogbogbo (GO, KEGG, ati bẹbẹ lọ) ati awọn apoti isura infomesonu ti ilọsiwaju (CARD, VFDB, bbl)
● Iwoye Jiome
A pese genome ni ọna kika fasta ti o wa ni imurasilẹ ati faili annotation genome (gff).
Jiini alaye - GO
Atọka Gene – CAZY carbohydrates
Jiini iworan - Circos Idite
Ṣawakiri awọn ilọsiwaju ti o ni irọrun nipasẹ awọn iṣẹ apejọ genome ti kokoro-arun BMKGene nipasẹ ikojọpọ awọn atẹjade ti a ṣe itọju.
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