16S / 18S / IT Amplicon Sequencing-NGS
Awọn ẹya ara ẹrọ Iṣẹ
● Syeed ti o tẹle: Illumina NovaSeq.
● Imudara ti awọn agbegbe kukuru ti 16S, 18S ati ITS, laarin awọn ifọkansi imudara miiran.
● Awọn aṣayan iyipada ti amplicon.
● Iriri iṣẹ akanṣe iṣaaju pẹlu awọn ibi-afẹde pupọ.
Awọn anfani Iṣẹ
●Laisi ipinya:Idanimọ iyara ti akopọ makirobia ni awọn apẹẹrẹ ayika.
●Ipinnu giga: Ni kekere-ọpọlọpọ irinše ni awọn ayẹwo ayika.
●Wulo jakejado: Oniruuru makirobia awujo-ẹrọ.
●Okeerẹ Bioinformatic Analysis: Apo QIIME2 tuntun (imọye titobi si ilolupo ẹda-ara) pẹlu awọn itupalẹ oniruuru ni awọn ofin ti data data, alaye, OTU/ASV.
●Amoye nla: Pẹlu 150 ẹgbẹrun ampilifaya atele ise agbese waiye lododun, BMKGENE mu lori kan mewa ti iriri, a gíga oye egbe onínọmbà, okeerẹ akoonu, ati ki o tayọ ranse si-tita support.
Awọn pato Iṣẹ
| Ile-ikawe | Ilana titele | Data niyanju |
| Amplicon | Illumina PE250 | Awọn ami 50/100/300K (Ka Awọn orisii) |
Awọn ibeere Iṣẹ
| Ifojusi (ng/µL) | Apapọ iye (ng) | Iwọn (µL) |
| ≥1 | ≥200 | ≥20 |
● Ile / sludge: 1-2g
● Awọn akoonu inu-ẹranko: 0.5-2g
● Awọn akoonu inu-kokoro: 0.1-0.25g
● Ohun ọgbin dada (erofo imudara): 0.1-0.5g
● Bakteria omitooro idarato erofo): 0.1-0.5g
● Faeces (ẹranko nla): 0.5-2g
● Faeces (eku): 3-5grains
● Omi iṣan alveolar ẹdọforo: iwe àlẹmọ
● Obo swab: 5-6 swabs
● Awọ ara / swab abe / itọ / asọ ti ẹnu / pharyngeal swab / rectal swab: 2-3 swabs
● Awọn microorganisms oju: iwe àlẹmọ
● Waterbody / air / biofilm: àlẹmọ iwe
● Endophytes: 1-2g
● Dental Plaque: 0.5-1g
Sisan Iṣẹ Iṣẹ
Ifijiṣẹ apẹẹrẹ
Ikole ìkàwé
Titele
Itupalẹ data
Lẹhin-tita awọn iṣẹ
Pẹlu itupale wọnyi:
- Aise data didara iṣakoso
- OTU iṣupọ /De-noise(ASV)
- OTU asọye
- Itupalẹ oniruuru Alpha: awọn atọka pupọ, pẹlu Shannon, Simpson ati ACE.
- Beta oniruuru onínọmbà
- Inter-ẹgbẹ onínọmbà
- Itupalẹ ibamu: laarin awọn ifosiwewe ayika ati akopọ OUT ati oniruuru
- Asọtẹlẹ jiini iṣẹ-ṣiṣe 16S
Histogram ti taxonomic pinpin
taxonomic opo clustering ooru map
Alfa oniruuru onínọmbà: rarefaction ti tẹ
Beta oniruuru onínọmbà: NMDS
Itupalẹ Intergroup: Awari biomarker LEFSE
Ṣawakiri awọn ilọsiwaju ti o ni irọrun nipasẹ awọn iṣẹ ṣiṣe atẹle amplicon BMKGene pẹlu Illumina nipasẹ ikojọpọ ti awọn atẹjade.
Dong, C. et al. (2022) 'Apejọ, Core Microbiota, ati Išė ti Rhizosphere Ile ati jolo Microbiota ni Eucommia ulmoides', Furontia ni Microbiology, 13. doi: 10.3389/FMICB.2022.855317/FULL.
Li, Y. et al. (2023) 'Synthetic bacterial consortia asopo fun itọju ti Gardnerella vaginalis-induced bacterial vaginosis in eku', Microbiome, 11(1), pp. 1-14. doi: 10.1186/s40168-023-01497-y
Yang, J., Fu, Y. ati Liu, H. (2022) 'Microbiomes ti air eruku ti a gba nigba ti ilẹ-orisun titi bioregenerative aye support ṣàdánwò "Lunar Palace 365"', Environmental Microbiomes, 17(1), pp. 1–20. doi: 10.1186 / S40793-022-00399-0 / awọn olusin / 8.
Yin, S. et al. (2022) 'Ọpọlọpọ-igbẹkẹle ifunni ifunni ti awọn jiini iṣẹ ṣiṣe ti o ni ibatan si iyipada nitrogen ti a ṣakoso pipadanu nitrogen ni idapọ’, Imọ-ẹrọ Bioresource, 361, p. 127678. doi: 10.1016 / J.BIORTECH.2022.127678.
