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Iimveliso

I-Whole genome bisulfite sequencing(WGBS)

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I-Whole Genome Bisulfite Sequencing (WGBS) ime njengendlela yomgangatho wegolide wokuhlola nzulu kwe-DNA methylation, ngokukodwa indawo yesihlanu kwi-cytosine (5-mC), umlawuli obalulekileyo we-gene expression kunye nomsebenzi weselula. Umgaqo ophantsi kwe-WGBS ubandakanya unyango lwe-bisulfite, ukuguqula ukuguqulwa kwe-cytosines engaxutywanga kwi-uracil (C ukuya ku-U), ngelixa ishiya i-cytosine ye-methylated ingatshintshi. Obu buchule bubonelela ngesisombululo esisisiseko esinye, sivumela abaphandi ukuba baphande ngokubanzi i-methylome kwaye bafumanise iipateni ze-methylation ezingaqhelekanga ezinxulumene neemeko ezahlukeneyo, ngakumbi umhlaza. Ngokusebenzisa i-WGBS, izazinzulu zinokufumana ulwazi olungenakuthelekiswa nanto kwi-genome-wide methylation landscapes, ukunika ukuqonda okucacileyo kweendlela ze-epigenetic eziphantsi kweenkqubo ezahlukeneyo zebhayoloji kunye nezifo.


Iinkcukacha zeNkonzo

Bioinformatics

Iziphumo zeDemo

Iimpapasho ezikhoyo

Iimpawu zeNkonzo

● Ifuna i-reference genome.

● I-Lambda DNA yongezwa ukujonga ukusebenza kakuhle kokuguqulwa kwe-bisulfite.

● Ulandelelwano kwi-Illumina NovaSeq.

Iinkonzo eziluncedo

Umgangatho weGolide woPhando lwe-DNA Methylation: Le teknoloji yokuguqulwa kwe-methylation evuthiweyo inokuchaneka okuphezulu kunye nokuphindaphinda okulungileyo.

UFundo oluBanzi kunye neSigqibo esiSinye:ukufunyanwa kweendawo ze-methylation kwinqanaba le-genome-wide.

Gqibezela iQonga:ukubonelela ngenkonzo egqwesileyo kwindawo enye ukusuka kulungiso lwesampulu, ukwakhiwa kwethala leencwadi, ulandelelwano ukuya kuhlalutyo lwe-bioinformatics.

UbuGcisa obuBanzi: kunye neeprojekthi zokulandelelana ze-WGBS ezigqitywe ngempumelelo kwiintlobo ezahlukeneyo zeentlobo, i-BMKGENE izisa ngaphezu kweminyaka elishumi yamava, iqela lokuhlalutya abanezakhono eziphezulu, umxholo obanzi, kunye nenkxaso ebalaseleyo emva kokuthengisa.

Ukubanakho ukujoyina kunye nohlalutyo lwe-Transcriptomics: ukuvumela uhlalutyo oludibeneyo lwe-WGBS kunye nezinye iinkcukacha ze-omics ezifana ne-RNA-seq.

IiNkcazo zeSampule

Ithala leencwadi

Isicwangciso solandelelwano

Imveliso yedatha ecetyiswayo

Ulawulo lwemeko

Bisulfite unyango

Illumina PE150

30x ubunzulu

Q30 ≥ 85%

Ukuguqulwa kweBisulfite > 99%

IiMfuno zeSampulu

 

Ugxininiso (ng/µL)

Imali iyonke (µg)

Iimfuno ezongezelelweyo

Genomic DNA

≥ 5

≥ 400 ng

Ukuthotywa okulinganiselweyo okanye ukosuleleka

Ukuhamba komsebenzi weNkonzo

ukuhanjiswa kwesampuli

Ukuhanjiswa kwesampuli

Ulingo lokulinga

Ukutsalwa kwe-DNA

ULungiselelo lweThala leeNcwadi

Ukwakhiwa kwethala leencwadi

Ukulandelelanisa

Ukulandelelanisa

Uhlalutyo lwedatha

Uhlalutyo lwedatha

数据上传-01

Ukuhanjiswa kwedatha


  • Ngaphambili:
  • Okulandelayo:

  • umfanekiso weskrini 4-01

    Ibandakanya uhlalutyo olulandelayo:

    ● Ulawulo lomgangatho wolandelelwano olukrwada;

    ● Ukwenza imephu yereferensi yegenome;

    ● Ukufunyanwa kweziseko ze-methylated ze-5mC;

    ● Uhlalutyo losasazo lwe-methylation kunye nenkcazo;

    ● Uhlalutyo lweeNgingqi ezineMethylated (DMRs);

    ● Inkcazo esebenzayo yeegenes ezinxulumene nee-DMRs.

    Ukufumanisa i-5mC ye-methylation: iintlobo zeendawo ze-methylated

     

    Umfanekiso we79

     

    Imephu ye-methylation. I-5mC methylation genome-wide distribution

     

    图片80

     

    Inkcazo yemimandla enemethylated kakhulu

    Umfanekiso we81

    Imimandla eyahlukeneyo yeMethylated: iijini ezihambelanayo

     

    Umfanekiso we82

     

    Imimandla eyahlukanisiweyo yeMethylated: inkcazo yemfuza ehambelana nayo (Gene Ontology)

     

    图片83

     

    Phonononga ukuqhubela phambili kophando oluququzelelwa yi-BMKGene yonke ye-genome bisulfite yolandelelwano lweenkonzo ngengqokelela egciniweyo yopapasho.

    Umlandeli, uY. et al. (2020) 'Uhlalutyo lweeprofayili ze-DNA methylation ngexesha lokuphuhliswa kwemisipha yegusha kusetyenziswa i-whole-genome bisulfite sequencing',I-BMC Genomics, 21(1), iphepha 1–15. doi: 10.1186/S12864-020-6751-5.

    Zhao, X. et al. (2022) 'Novel deoxyribonucleic acid methylation perturbations kubasebenzi abavezwe kwivinyl chloride',I-Toxicology kunye neMpilo yoShishino, 38(7), iphepha 377–388. doi: 10.1177/07482337221098600

    Zuo, J. et al. (2020) 'Ubudlelwane phakathi kwe-genome methylation, amanqanaba e-RNAs angenayo ikhowudi, i-mRNAs kunye ne-metabolites ekuvuthweni kweziqhamo ze-tomato',Ijenali yezityalo, 103(3), iphepha 980-994. doi: 10.1111/TPJ.14778.

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