● Ifuna i-reference genome.
● I-Lambda DNA yongezwa ukujonga ukusebenza kakuhle kokuguqulwa kwe-bisulfite.
● Ulandelelwano kwi-Illumina NovaSeq.
●Umgangatho weGolide woPhando lwe-DNA Methylation: Le teknoloji yokuguqulwa kwe-methylation evuthiweyo inokuchaneka okuphezulu kunye nokuphindaphinda okulungileyo.
●UFundo oluBanzi kunye neSigqibo esiSinye:ukufunyanwa kweendawo ze-methylation kwinqanaba le-genome-wide.
●Gqibezela iQonga:ukubonelela ngenkonzo egqwesileyo kwindawo enye ukusuka kulungiso lwesampulu, ukwakhiwa kwethala leencwadi, ulandelelwano ukuya kuhlalutyo lwe-bioinformatics.
●UbuGcisa obuBanzi: kunye neeprojekthi zokulandelelana ze-WGBS ezigqitywe ngempumelelo kwiintlobo ezahlukeneyo zeentlobo, i-BMKGENE izisa ngaphezu kweminyaka elishumi yamava, iqela lokuhlalutya abanezakhono eziphezulu, umxholo obanzi, kunye nenkxaso ebalaseleyo emva kokuthengisa.
●Ukubanakho ukujoyina kunye nohlalutyo lwe-Transcriptomics: ukuvumela uhlalutyo oludibeneyo lwe-WGBS kunye nezinye iinkcukacha ze-omics ezifana ne-RNA-seq.
Ithala leencwadi | Isicwangciso solandelelwano | Imveliso yedatha ecetyiswayo | Ulawulo lwemeko |
Bisulfite unyango | Illumina PE150 | 30x ubunzulu | Q30 ≥ 85% Ukuguqulwa kweBisulfite > 99% |
Ugxininiso (ng/µL) | Imali iyonke (µg) | Iimfuno ezongezelelweyo | |
Genomic DNA | ≥ 5 | ≥ 400 ng | Ukuthotywa okulinganiselweyo okanye ukosuleleka |
Ibandakanya uhlalutyo olulandelayo:
● Ulawulo lomgangatho wolandelelwano olukrwada;
● Ukwenza imephu yereferensi yegenome;
● Ukufunyanwa kweziseko ze-methylated ze-5mC;
● Uhlalutyo losasazo lwe-methylation kunye nenkcazo;
● Uhlalutyo lweeNgingqi ezineMethylated (DMRs);
● Inkcazo esebenzayo yeegenes ezinxulumene nee-DMRs.
Ukufumanisa i-5mC ye-methylation: iintlobo zeendawo ze-methylated
Imephu ye-methylation. I-5mC methylation genome-wide distribution
Inkcazo yemimandla enemethylated kakhulu
Imimandla eyahlukeneyo yeMethylated: iijini ezihambelanayo
Imimandla eyahlukanisiweyo yeMethylated: inkcazo yemfuza ehambelana nayo (Gene Ontology)
Phonononga ukuqhubela phambili kophando oluququzelelwa yi-BMKGene yonke ye-genome bisulfite yolandelelwano lweenkonzo ngengqokelela egciniweyo yopapasho.
Umlandeli, uY. et al. (2020) 'Uhlalutyo lweeprofayili ze-DNA methylation ngexesha lokuphuhliswa kwemisipha yegusha kusetyenziswa i-whole-genome bisulfite sequencing',I-BMC Genomics, 21(1), iphepha 1–15. doi: 10.1186/S12864-020-6751-5.
Zhao, X. et al. (2022) 'Novel deoxyribonucleic acid methylation perturbations kubasebenzi abavezwe kwivinyl chloride',I-Toxicology kunye neMpilo yoShishino, 38(7), iphepha 377–388. doi: 10.1177/07482337221098600
Zuo, J. et al. (2020) 'Ubudlelwane phakathi kwe-genome methylation, amanqanaba e-RNAs angenayo ikhowudi, i-mRNAs kunye ne-metabolites ekuvuthweni kweziqhamo ze-tomato',Ijenali yezityalo, 103(3), iphepha 980-994. doi: 10.1111/TPJ.14778.