TGuide Smart DNA Ikhithi yokuCoca
Inombolo yekhathalogu / ukupakishwa
| Ikati. Hayi | ID | Inani lamalungiselelo |
| 4993550(ikhatriji) | DP642-DE | 48 |
Isikhokelo sokukhetha i-Reagent ye-TGuide S16
| Nuclei Acid | Uhlobo lweSampuli | Igama lemveliso | Ubungakanani bokupakisha (preps) Ikhatriji/ ipleyiti | Inombolo yekati. Ikhatriji/ipleyiti |
| DNA | Inyama yezilwanyana | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Isityalo kunye neMbewu | TGuide Smart Magnetic Plant DNA Kit | 48 | 4993548 |
| DNA | Umhlaba/Isitulo | TGuide Smart Soil / Stool DNA Kit | 48 | 4993549 |
| DNA | Iimveliso zeGel & PCR | TGuide Smart DNA Ikhithi yokuCoca | 48 | 4993550 |
| DNA | IGazi Elipheleleyo | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Iseli/iSwab/Amabala omileyo, njalo njalo | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| I-RNA | Igazi/Iseli/Izicubu | TGuide Smart Blood/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| I-RNA | Isityalo kunye neMbewu | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | Ulwelo lwasimahla lweSeli (iSerum, njalo njalo) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Intshayelelo yemveliso
Ibuyisela i-DNA ngokukhawuleza nangempumelelo kwijeli ye-TAE/TBE kunye nemveliso ye-PCR.
Uluhlu lobungakanani beqhekeza le-DNA: 100 bp-15 kb.
I-DNA efunyenwe ngokusebenzisa le khithi ingasetyenziselwa ukugaya, i-PCR, ulandelelwano, ukuhlolwa kwethala leencwadi, ukudibanisa, ukuguqulwa kunye nezinye iimvavanyo.
Iimbonakalo
Kulula kakhulu ukuyisebenzisa
Akukho msebenzi owongezelelweyo wokubhobhoza.
Umthamo we-elution unokuhlengahlengiswa njengoko kufuneka.
Ufakelo kunye nokusebenza kufuna uqeqesho oluncinci. Ngeenkqubo ezifakwe ngaphambili, khupha i-cartridge, khetha iprotocol kwaye usebenzise uvavanyo lwakho. Iinkqubo zikulungele ukusetyenziswa, kwaye ziyakwazi ukwenziwa.
Ii-reagents ezilayishwe kwangaphambili kunye nezinto ezisetyenziselwa ukulahla.
Ukuphathwa kweekhemikhali kuyapheliswa ukuya kwinqanaba elikhulu lokuqinisekisa ukhuseleko lokusebenza.
Iziphumo zeDemo
I-300 bp segment ye-agarose gel DNA yokuhlanjululwa kunye nokubuyisela
Uxinzelelo lwejeli yeAgarose :1.5%(TBE)
Iyalayisha
umthamo: 8 μl
Umakisha: 50 bp DNA ileli, TIANGEN
I-S16-01, i-S16-02, kunye ne-S16-03 imele isampuli ehambelanayo yokucoca kunye novavanyo lokubuyisela usebenzisa i-3 TGuide S16 Nucleic Acid Extractors kunye ne-TGuide Smart DNA Purification Kit (4993550) ngexesha elifanayo.
Inkampani: ikhithi yotsalo lwekholamu yesandla yohlobo olwaziwayo
I-2000 bp segment ye-agarose gel DNA yokuhlanjululwa kunye nokubuyisela
Uxinzelelo lwejeli ye-Agarose :1.5% (TBE)
Ukulayisha umthamo: 8 μl
Umakisha: D2000, TIANGEN
I-S16-01, i-S16-02, kunye ne-S16-03 imele ukuhlanjululwa kwesampulu efanayo kunye nokubuyisela
ulingelo usebenzisa i-3 TGuide S16 Nucleic Acid Extractors nge-TGuide Smart DNA Purification Kit (4993550) ngexesha elinye.
Inkampani: ikhithi yotsalo lwekholamu yesandla yohlobo olwaziwayo
Isiphumo sovavanyo: usebenzisa i-TGuide S16 Nucleic Acid Extractor kunye nekhithi ekhethekileyo ye-TGuide Smart DNA Purification Kit (4993550) inokufezekisa i-automation epheleleyo yokubuyisela i-gel. Ukuhambelana kokuhlanjululwa kunye nokubuyisela kuhle kakhulu, kwaye isiphumo sokutsalwa singcono kuneemveliso ezikhuphisanayo zezinye iinkampani.
I-15000 icandelo le-bp i-agarose gel DNA recovery kunye ne-PCR yokucoca imveliso
Uxinzelelo lwejeli yeAgarose :1%(TBE)
Ukulayisha umthamo: 3 μl
Isiphawuli:
D15000,TIANGEN
G1, G2: ukubuyiswa kwejeli ye-agarose
C1:Ukucoca imveliso yePCR.
Iprothokholi yeManuwali: ikhithi yotsalo lwekholamu ye-manual ye-brand eyaziwayo B
Inkampani: indlela yentsimbi enozibuthe -ikhithi yokutsalwa esekwe kwibrendi eyaziwayo
Isiphumo sovavanyo: usebenzisa i-TGuide S16 Nucleic Acid Extractor enekhithi ye-TGuide Smart DNA Purification Kit (4993550), ukubuyiswa kwejeli ye-agarose kunye nokuhlanjululwa kwemveliso ye-PCR kunokufezekiswa ngokuzenzekelayo, kwaye umphumo wokucoca ufaniswe nalawo yikholomu ye-spin. ukuhlanjululwa okusekelwe.
