Temuide s16 i-nucleic acid isikhupheli
Intshayelelo Intshayelelo
I-TGEID S16 I-NCECEID Acictor inokuqhuba ukuya kwiisampulu ezili-16 ngaxeshanye ngaxeshanye nge-magnetic rods yokubopha kwaye idluliselwe kwi-Beagec Beds, i-96-ye-PAYPIC ye-TAPRICRGE ye-cartic kunye ne-cartic ye-carticker ye-cartic kunye ne-cartic ye-carticker kunye nemagnethi ye-cartic kunye ne-cignic cartridge kunye nomqhinga we-cartic. Ngokusebenzisa i-reacid ye-acid ehambelanayo, le mveliso inokuhlambulula iDNA / RNA kwiisampulu ezahlukeneyo zesilwanyana, isityalo, intsholongwane, i-fucteria, i-fucterid ges kunye nemveliso ye-Agarose.
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Iimbonakalo
Eyona nto ilula ukuyisebenzisa
Akukho msebenzi wongezelelweyo wepayitha. Ivolumu ye-LELTOS inokuhlengahlengiswa njengoko kufuneka.
Ukufakwa kunye nokusebenza kufuna uqeqesho oluncinci. Ngeenkqubo ezifakwe kwangaphambili, zikhuphe i-cartridge, khetha iprotocol kwaye uqhube ilize. Iinkqubo zilungele ukusetyenziswa, kwaye zinokufihlakala.
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Imowudi entsha yokubopha
I-magnetic rod yamkela imowudi yokuphela kwendawo eqinileyo.
Iibhlorho zemagneti zibopha ngasezantsi, nto leyo eqinisekisa ukuba i-Eueent isenokufihla yonke imigudu ye-magnetic naxa ivolumu ye-Euenet incinci. Uyilo lukhulisa isivuno se-nucleic acid.
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Uyilo olugqibeleleyo lwetanki lokufudumeza
Kukho ikholamu efakwe phakathi kwe-luffer buffer kunye nekholamu ye-EUEGN, ethintela ngokufanelekileyo i-Eeuent ekwi-Evauped ngenxa yokufudumeza kwekholam. Ke ngoko, ubungakanani bokubuyiswa kwesisombululo se-nucleic sichane ngakumbi.
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Ulawulo longcoliseko kunye nokukhuselwa komqhubi
Ingabekwa kwibhentshi ecocekileyo okanye kwi-hood yemichiza.
Isixhobo sisebenza ngendlela ezenzekelayo nevaliweyo.
Inkqubo yolawulo olungcolisayo yamkela iindlela ezimbini: Inkqubo yolawulo lwangaphakathi lokungcoliseka kwenkqubo ye-Ultraviolet yeSterilizolet, ukuze kuthintelwe kakhulu ungcoliseko olunqamlezileyo phakathi kweekholamu nakwiisampulu.
I-Regagents yakhawulezisa i-Regegements kunye neeNtengiso eziNgezelelweyo.
Ukuphatha kwamachiza kuyasuswa kwelona nqanaba lokuqinisekisa ukhuseleko.
Iziphumo
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Isiphumo sovavanyo
Ukukhupha ngokuzenzekelayo i-DNA ye-gemic yesampulu ezininzi kubandakanya iiseli, izicubu, igazi, igazi elomileyo (i-DBS), iLactobacilsus, i-Lactoli, i-Swab, iphumeze ubunyulu obulungileyo kunye nobunyulu obuphezulu.
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I-OD260 / OD280 malunga ne-1.7 ~ 1.9, kwaye
I-OD260 / OD230> 1.6.
Umthamo we-LELT: 100 μl
I-agarose gel yoxinzelelo: 1.5%
Umthamo: 1 μl
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Sebenzisa i-guide s16 nge-regent ye-vient ye-viide ye-v pheral vna / rna kit (4993702)
Ukususa ngokuzenzekelayo i-DNA / RNA evela kwimithombo emininzi kubandakanya igazi liphela, i-Swab kunye nePlasma yafumana isivuno esihle kunye nobunyulu obuphezulu. -
I-OD260 / I-OD280 ijikeleze i-1.7 ~ 1.9, kunye ne-OD260 / OD20> 1.6 .6.
Inkampani & B: Iimpawu eziziwayo.
I-ND: Isifo seNewcastle, H5: Umkhuhlane we-Avian, CP: I-Can-Canvovirus, i-ASF: intsholongwane ye-feever yase-Afrika
Ubuncinci bovakalelo lunokufikelela kwiikopi eziyi-100 / ml.
Isiphumo sokuvavanya
I-OD260 / OD280 malunga ne-1.7 ~ 1.9, kunye ne-OD260 / OD20> 1.7.
Isampulu yesampulu: iisampulu zegazi ezithathu zegazi. Iimpindezelo ezintathu ziimvavanyo zeekhithi nganye.
Iphindaphindeka kwikhithi nganye ivela kumthombo ongumzekelo omnye.
Imeko yesampulu: igazi elinomkhenkce
I-POLUMPUSUP: 200 μl
Umthamo we-LELT: 100 μl
I-agarose gel yoxinzelelo: 1.5%
Umthamo: 1 μl
M: UMarker III, Tiangen
Inkampani & B inkampani: Iimpawu ezikhoyo
Ngo-2000 BP Segement Agarose Garose Gel DNA kunye nokuBuyiswa kwakhona
I-agarose gel yoxinzelelo: 1.5% (TBE)
Umthamo: 8 μl
UMarker: D2000, Tiangen
S16-01, S16-02, kunye ne-S16-03 imele ukuphononongwa kwesampula kunye nokubuyiswa kwe-TGEID S16 I-STEID S16 I-499350) ngaxeshanye.
Inkampani: Incwadana yencwadi yendawo yekholamu yeKholamu yeKit yoThengisi
I-Aguity 2100 isetyenziselwa ukuguqulwa kwe-RNA ingqibelelo yeRNA.
Isampulu yesampulu: i-cd-1 yeempuku
Imeko yesampulu: igazi elitsha
Ubungakanani besampulu: 200 μl
Isiphumo sokuvavanya
Umthamo: 1 μl. Isibindi sasihlutha izihlandlo ezi-5 kwaye silayishwe. I-Agarose Gel yoxinzelelo: 1%. I-elektrophoresis ye-20 min nge-6 v / cm;
M: tiangen marker III
1-4: Intliziyo 5-8: IPHEPHA 9-12: I-LUNG 13-16: Izintso
Iisampulu ezimbini zokuqala zezicubu zazo zakhutshwe yikhithi yokukhupha ikholamu yekholamu, kwaye ezimbini zokugqibela zikhutshiwe ngokuzenzekelayo yi-TGEID S16.
Isampulu yesampulu: i-cd-1 yeempuku
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Isiphumo sokuvavanya Ukucoceka kuyafana, nge-OD260 / OD280 ngeenxa zonke 1.7 ~ 1.9 kunye ne-OD260 / I-OD20> 1.7. Ukuqukumbela, i-Tgeide S16 ingasetyenziselwa ukukhupha i-DNA ye-Wemic kwizicubu zezilwanyana xa ingeniswe isisombululo sekholamu ye-SPIN.
Iyonke yokukhupha i-RNA iyonke kwiisampulu zezityalo ezahlukeneyo
Ubungakanani besampulu: i-100 mg
Isampulu yangaphambi konyango: Iqondo lobushushu eliphantsi
I-Agarose Gel yoxinzelelo: 1% (TAE)
Umthamo: 1 μl
M: UMarker III, Tiangen
1-4: Imbewu yengqolowa 5-8: I-Corn Amagqabi 9-12: I-cabbiage Roots 13-16: I-Soybebbeans
Iisampulu ezimbini zokuqala zakhutshwa yikhithi yokukhupha ikholamu kunye neesampulu ezimbini zokugqibela zakhutshwa yi-TGIide S16.
Ukukhutshelwa kwe-DNA ye-GNA yeengcambu zengqolowa
Ubungakanani besampulu: i-100 mg
Isampulu yokuphathwa kwangaphambili: Ukugaya nge-nitrogen ye-nitrogen okanye i-tissue ye-tissue ye-humogenizer
I-Agarose Gel yoxinzelelo: 1% (TAE)
Umthamo: 2 μl
UMarker: D15000, Tiangen
Inkampani: uphawu olwaziwayo
Isiphumo sokuvavanya Ngapha koko, ingqibelelo ye-DNA ye-gemic ilunge ngakumbi kunezo zeemveliso ezikhuphisana nezinye iinkampani. Kweli xesha lovavanyo, malunga ne-15 μg ye-nucleid ikhutshiwe kwi-100 mg ye-In Eats, nge-OD260 / OD280 ngeenxa zonke 1.8 ~ 430 / AD20> 2.0.
