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条形 isibhengezo-03

Iimveliso

I-Aussing Amusis eQhelekileyo ye-Amqueding (Slaf-Seq)

I-genotyping ephezulu yendlela, ngakumbi kwimigangatho emikhulu yokuphila, linyathelo elisisiseko kwimibutho yemfuza kwaye ibonelela ngesiseko semfuza yokufunyanwa kwemfuza, njl njl. Njl.Ukucuthwa kokumelwa kwe-genome ye-genome (i-RRGS)ihlala isebenza kwezi zifundo ukunciphisa iindleko zokulandelela isampulu kwisimo ngelixa ugcina ukwenziwa ngokuqiqayo kwimfuza yokumakisha imfuza. I-RRGS izuze oku ngokugayisela i-DNA ye-enzymes ye-minzy kwaye igxile kubungakanani obuthile obuqhetseba, ngaloo ndlela kulandelwa kuphela iqhekeza le-genome. Phakathi kweendlela ezahlukeneyo ze-RRGs, i-tuessis-tues ye-Aussus ye-Auis ye-Auism Le ndlela, iphuhlisiwe ngokuzimeleyo yi-Bmkgene, iqinisekisile i-entermition ye-enzyme imiselwe kuyo yonke iprojekthi. Oku kuqinisekisa ukuveliswa kwenani elininzi leemegi (400-500 ze-BPS ze-genome) ekusasazwe kuyo i-genome genome, ngaloo ndlela uqinisekisa ngempumelelo i-genome, ngaloo ndlela iqinisekisa olona phawu lubalaseleyo lwemfuza.


Iinkcukacha zenkonzo

I-Bioinformatics

Iziphumo

Ushicilelo olubonisiweyo

Umsebenzi wokusebenza

图片 31

Iskimu sobugcisa

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Iimpawu zenkonzo

● Ukulandelelana kwi-novaseq ngePe150.

● Ukulungiselela kwethala lencwadi ngebhakhowudi kabini, ivuselela iphuli kwiisampulu ezingaphezu kwe-1000.

● Obu buchule bungasetyenziswa okanye ngaphandle kwereferensi ye-genome, ngemibhobho yebhayiloji eyahlukeneyo yetyala ngalinye:

Ngereferensi ye-genome: i-snp kunye nokufunyanwa kwe-Indel

Ngaphandle kweRekhodi ye-Genome: Iqela lesampulu kunye nokufunyanwa kwe-SNP

● KwieSilicoInqanaba lokuyilwa kwangaphambili kothintelo lwee-enzyme izithintelo ze-enzyme zihlolisile ukuze zifumane ezo zivelisa ukusasazwa kwe-slaf iimpawu ecaleni kwe-genome.

● Ngexesha lokuvavanywa kwangaphambili, ukudityaniswa ezintathu ze-enzyme kuvavanywa kwiisampuli ezi-3 ukuvelisa amathala eencwadi ama-3, kwaye olu lwazi lusetyenziswa ukukhetha umdibaniso we-enzyme opheleleyo weprojekthi.

Izibonelelo zeNkonzo

Ukufunyanwa kwemfuza ephezulu: Ukudibanisa inkqubo yokungena kwi-becode yebhakhowudi ivumela ukulandelelana kwabantu abaninzi, kwaye i-amuni eyodwa yokuphucula ukusebenza kakuhle, ukuqinisekisa ukuba iinombolo zethegi ziyahlangabezana neemfuno ezahlukeneyo zemibuzo yophando.

 Ukuxhomekeka okuncinci kwi-genome: Ingasetyenziselwa iintlobo kunye okanye ngaphandle kwereferensi ye-genome.

Uyilo lweNkqubo eguqukayo: I-enzyme-enzyme, i-enzyme, i-enzyme, i-enzyme ye-enzyme, kwaye iintlobo ngeentlobo ze-enzymes zinokuthi zikhethwe ukuba zibonelele ngeenjongo zophando ezahlukeneyo zophando okanye iintlobo. IeSilicoUyilo lwangaphambi luqhutywa ukuqinisekisa uyilo lwe-enzyme.

 Ukusebenza kakuhle kwi-enzymatic diglovetion: Ukuqhubela phambili kweeSilicoUyilo lwangaphambi kokuqinisekiswa uyilo olugqibeleleyo ngokusasaza ii-tag ze-slaf kwi-chromosome (1 slaf tag / 4kb) kunye nokuncitshiswa kolandelelwano (<5%).

Ubuchule obubanzi: Iqela lethu lizisa ubutyebi bamava kuzo zonke iiprojekthi, ngerekhodi yokuvala ngaphezulu kwe-5000 yeeprojekthi ze-SLAF-Seq-SEALS, izinambuzane, kunye nezinto eziphilayo, kunye nezinambuzane.

 Ukuziqhelanisa nokusebenza kwe-biineformatic: I-Bmkgene iphuhlise indlela edityanisiweyo yokudityaniswa kwe-biof-seq ukuqinisekisa ukuthembeka kunye nokuchaneka kwemveliso yokugqibela.

 

Inkcazo yenkonzo

 

Uhlobo lohlalutyo

Umgangatho ocetyiswayo

Isicwangciso sokulandelelana

Ubunzulu bokulandelelana kwe-tag

Inombolo yeTag

Imephu yemfuza

2 Bazali kunye >> imbewu eyi-150

Abazali: 20x wgs

I-EPPONICT: I-10X

Ubungakanani be-genome:

<400 MB: WG ziyacetyiswa

<1GB: I-100k ye-100k

I-1-2GB :::::::::

> I-2GB: Iithegi ze-300K

I-max 500k

Izifundo ze-Genome-ngokubanzi (Gwaka)

Iisampulu

I-10x

Indalo yemfuza

Iisampulu ze-30, kunye neeSampuli ezili-10 kwiQela ngalinye

I-10x

Iimfuneko zeNkonzo

Uxinzelelo ≥ 5 NG / μl

Iyonke imali ≥ 80 ng

I-Nanodrop OD260 / 280 = 1.6-2.5

I-Agarose Gel: Hayi okanye i-Degrades okanye ungcoliseko

Ukuhanjiswa kwesampulu

Isikhongozeli: 2 Ml Centrifuge Tube

(Uninzi lweesampulu, sicebisa ukuba singayigcini e-Ethanol)

Imeyibheli ilebheli: Iisampulu kufuneka zibhalwe ngokucacileyo kwaye zize zingenise ifom yengcaciso yolwazi.

Ukuhanjiswa: Umkhenkce owomileyo: Iisampulu kufuneka zipakishwe kwiingxowa kuqala kwaye zingcwatywe kwi-ice eyomileyo.

Inkonzo yenkonzo

Isampulu qc
Uvavanyo lwePilot
I-Slaf Uvavanyo
Ukulungiswa kwethala leencwadi
Ukulandelelana
Uhlalutyo lwedatha
Emva kokuthengisa iinkonzo

Isampulu qc

Uvavanyo lwePilot

I-Slaf

Ukulungiswa kwethala leencwadi

Ukulandelelana

Uhlalutyo lwedatha

Iinkonzo zentengiso emva


  • Ngaphambili:
  • Okulandelayo:

  • 图片 32Kubandakanya nohlalutyo olulandelayo:

    • Ukulandelelana kwe-qc
    • I-Slaf Pemart Uphuhliso

    Imephu kwireferensi ye-genome

    Ngaphandle kwesalathiso se-genome: dibanisa

    • Uhlalutyo lweempawu ze-slaf. Iinkcukacha-manani, ukuhanjiswa kwi-genome nganye
    • Ukufunyanwa kweMarker: I-SNP, i-Indel, i-SNV, CV

    Ukuhanjiswa kweempawu ze-Slaf kwi-chromosomes:

     图片 33

     

    Ukuhanjiswa kwe-SNPs kwi-chromosomes:

     34Isichazi-magama

    图片 35

     

    Unyaka

    Ijenali

    IF

    Isihloko

    Izicelo

    2022

    Unxibelelwano lwendalo

    17.694

    Isiseko se-gemic se-giga-chromosomes naseGiga-Genome yomthi

    I-Paeonia Ostiii

    I-slaf-gwas

    Ngo-2015

    I-phytologist entsha

    7.433

    Imimandla ye-ECHEDICRECRORDE ye-APHONICACIC ye-Agronomic ukubaluleka

    iimbotyi zesoya

    I-slaf-gwas

    2022

    Ijenali yoPhando oluPhambili

    12.822

    I-Genome-i-Itrass Endet ye-Gosypium ye-gosyypium kwi-G. Hirsutum

    Chaza i-loirior elona candelo liphuculwe ngaxeshanye lomgangatho we-cotton fiber kunye nesivuno

    imikhwa

    I-Slaf-Evelics Genetics

    2019

    Isityalo se-molecular

    10.81

    Uhlalutyo lwabemi beWemic kunye ne-NOVO I-DEOVI CWON IVIKELELO YOKUZIPHATHA

    Irayisi njengomdlalo wendaleko

    I-Slaf-Evelics Genetics

    2019

    Indalo yemfuza

    31.616

    Ukulandelelana kwe-genome kunye neesahluko zemfuza ze-carp eqhelekileyo, iCyprinus Carpio

    Imephu yokunxibelelana ye-Slaf

    Ngo-2014

    Indalo yemfuza

    25.455

    I-genome ye-peanut etyaliweyo ibonelela ngengqondo ye-leary ye-karyotpes, polyploid

    Indaleko kunye nendlu yendlu yendlu.

    Imephu yokunxibelelana ye-Slaf

    2022

    Ijenali yeBiotechnology

    9.803

    Ukuchonga i-ST1 ityhila ukhetho olubandakanya ukubetha kwe-morphology yembewu

    kunye nomxholo weoyile ngexesha leSoybean

    Ukuphuhliswa kweMakishi yeSlaf

    2022

    Ijenali yeHlabathi yeNzululwazi yezeNdalo

    6.208

    Ukuchongwa kunye ne-DNA Marker yeNdawo yeNdawo yeMollis 2ns (2D)

    I-dismomomontle elungileyo

    Ukuphuhliswa kweMakishi yeSlaf

     

    Unyaka

    Ijenali

    IF

    Isihloko

    Izicelo

    2023

    Imida kwiSayensi yeSityalo

    6.735

    Imephu ye-Qtl kunye nohlalutyo lwe-screptriptome ngomxholo weswekile ngexesha lokuvuthwa kwePyrus Pyrifolia

    Imephu yemfuza

    2022

    Ijenali yeBiotechnology

    8.154

    Ukuchonga i-ST1 ityhila ukhetho olubandakanya ukubetha kwe-hitchhilogy morphology kunye nomxholo weoyile ngexesha lesoben

     

    Ukufowuna kwe-SNP

    2022

    Imida kwiSayensi yeSityalo

    6.623

    Imephu ye-genome-ngokubanzi yemephu ye-dunotypes ezinqabileyo kwimo yesazela.

     

    I-gwas

    Fumana isicatshulwa

    Bhala umyalezo apha kwaye uthumele kuthi

    Thumela umyalezo wakho kuthi: