● Ukulandelelana kwi-novaseq ngePe150.
● Ukulungiselela kwethala lencwadi ngebhakhowudi kabini, ivuselela iphuli kwiisampulu ezingaphezu kwe-1000.
● Obu buchule bungasetyenziswa okanye ngaphandle kwereferensi ye-genome, ngemibhobho yebhayiloji eyahlukeneyo yetyala ngalinye:
Ngereferensi ye-genome: i-snp kunye nokufunyanwa kwe-Indel
Ngaphandle kweRekhodi ye-Genome: Iqela lesampulu kunye nokufunyanwa kwe-SNP
● KwieSilicoInqanaba lokuyilwa kwangaphambili kothintelo lwee-enzyme izithintelo ze-enzyme zihlolisile ukuze zifumane ezo zivelisa ukusasazwa kwe-slaf iimpawu ecaleni kwe-genome.
● Ngexesha lokuvavanywa kwangaphambili, ukudityaniswa ezintathu ze-enzyme kuvavanywa kwiisampuli ezi-3 ukuvelisa amathala eencwadi ama-3, kwaye olu lwazi lusetyenziswa ukukhetha umdibaniso we-enzyme opheleleyo weprojekthi.
●Ukufunyanwa kwemfuza ephezulu: Ukudibanisa inkqubo yokungena kwi-becode yebhakhowudi ivumela ukulandelelana kwabantu abaninzi, kwaye i-amuni eyodwa yokuphucula ukusebenza kakuhle, ukuqinisekisa ukuba iinombolo zethegi ziyahlangabezana neemfuno ezahlukeneyo zemibuzo yophando.
● Ukuxhomekeka okuncinci kwi-genome: Ingasetyenziselwa iintlobo kunye okanye ngaphandle kwereferensi ye-genome.
●Uyilo lweNkqubo eguqukayo: I-enzyme-enzyme, i-enzyme, i-enzyme, i-enzyme ye-enzyme, kwaye iintlobo ngeentlobo ze-enzymes zinokuthi zikhethwe ukuba zibonelele ngeenjongo zophando ezahlukeneyo zophando okanye iintlobo. IeSilicoUyilo lwangaphambi luqhutywa ukuqinisekisa uyilo lwe-enzyme.
● Ukusebenza kakuhle kwi-enzymatic diglovetion: Ukuqhubela phambili kweeSilicoUyilo lwangaphambi kokuqinisekiswa uyilo olugqibeleleyo ngokusasaza ii-tag ze-slaf kwi-chromosome (1 slaf tag / 4kb) kunye nokuncitshiswa kolandelelwano (<5%).
●Ubuchule obubanzi: Iqela lethu lizisa ubutyebi bamava kuzo zonke iiprojekthi, ngerekhodi yokuvala ngaphezulu kwe-5000 yeeprojekthi ze-SLAF-Seq-SEALS, izinambuzane, kunye nezinto eziphilayo, kunye nezinambuzane.
● Ukuziqhelanisa nokusebenza kwe-biineformatic: I-Bmkgene iphuhlise indlela edityanisiweyo yokudityaniswa kwe-biof-seq ukuqinisekisa ukuthembeka kunye nokuchaneka kwemveliso yokugqibela.
Uhlobo lohlalutyo | Umgangatho ocetyiswayo | Isicwangciso sokulandelelana | |
Ubunzulu bokulandelelana kwe-tag | Inombolo yeTag | ||
Imephu yemfuza | 2 Bazali kunye >> imbewu eyi-150 | Abazali: 20x wgs I-EPPONICT: I-10X | Ubungakanani be-genome: <400 MB: WG ziyacetyiswa <1GB: I-100k ye-100k I-1-2GB ::::::::: > I-2GB: Iithegi ze-300K I-max 500k |
Izifundo ze-Genome-ngokubanzi (Gwaka) | Iisampulu | I-10x | |
Indalo yemfuza | Iisampulu ze-30, kunye neeSampuli ezili-10 kwiQela ngalinye | I-10x |
Uxinzelelo ≥ 5 NG / μl
Iyonke imali ≥ 80 ng
I-Nanodrop OD260 / 280 = 1.6-2.5
I-Agarose Gel: Hayi okanye i-Degrades okanye ungcoliseko
Isikhongozeli: 2 Ml Centrifuge Tube
(Uninzi lweesampulu, sicebisa ukuba singayigcini e-Ethanol)
Imeyibheli ilebheli: Iisampulu kufuneka zibhalwe ngokucacileyo kwaye zize zingenise ifom yengcaciso yolwazi.
Ukuhanjiswa: Umkhenkce owomileyo: Iisampulu kufuneka zipakishwe kwiingxowa kuqala kwaye zingcwatywe kwi-ice eyomileyo.
Imephu kwireferensi ye-genome
Ngaphandle kwesalathiso se-genome: dibanisa
Ukuhanjiswa kweempawu ze-Slaf kwi-chromosomes:
Ukuhanjiswa kwe-SNPs kwi-chromosomes:
Unyaka | Ijenali | IF | Isihloko | Izicelo |
2022 | Unxibelelwano lwendalo | 17.694 | Isiseko se-gemic se-giga-chromosomes naseGiga-Genome yomthi I-Paeonia Ostiii | I-slaf-gwas |
Ngo-2015 | I-phytologist entsha | 7.433 | Imimandla ye-ECHEDICRECRORDE ye-APHONICACIC ye-Agronomic ukubaluleka iimbotyi zesoya | I-slaf-gwas |
2022 | Ijenali yoPhando oluPhambili | 12.822 | I-Genome-i-Itrass Endet ye-Gosypium ye-gosyypium kwi-G. Hirsutum Chaza i-loirior elona candelo liphuculwe ngaxeshanye lomgangatho we-cotton fiber kunye nesivuno imikhwa | I-Slaf-Evelics Genetics |
2019 | Isityalo se-molecular | 10.81 | Uhlalutyo lwabemi beWemic kunye ne-NOVO I-DEOVI CWON IVIKELELO YOKUZIPHATHA Irayisi njengomdlalo wendaleko | I-Slaf-Evelics Genetics |
2019 | Indalo yemfuza | 31.616 | Ukulandelelana kwe-genome kunye neesahluko zemfuza ze-carp eqhelekileyo, iCyprinus Carpio | Imephu yokunxibelelana ye-Slaf |
Ngo-2014 | Indalo yemfuza | 25.455 | I-genome ye-peanut etyaliweyo ibonelela ngengqondo ye-leary ye-karyotpes, polyploid Indaleko kunye nendlu yendlu yendlu. | Imephu yokunxibelelana ye-Slaf |
2022 | Ijenali yeBiotechnology | 9.803 | Ukuchonga i-ST1 ityhila ukhetho olubandakanya ukubetha kwe-morphology yembewu kunye nomxholo weoyile ngexesha leSoybean | Ukuphuhliswa kweMakishi yeSlaf |
2022 | Ijenali yeHlabathi yeNzululwazi yezeNdalo | 6.208 | Ukuchongwa kunye ne-DNA Marker yeNdawo yeNdawo yeMollis 2ns (2D) I-dismomomontle elungileyo | Ukuphuhliswa kweMakishi yeSlaf |
Unyaka | Ijenali | IF | Isihloko | Izicelo |
2023 | Imida kwiSayensi yeSityalo | 6.735 | Imephu ye-Qtl kunye nohlalutyo lwe-screptriptome ngomxholo weswekile ngexesha lokuvuthwa kwePyrus Pyrifolia | Imephu yemfuza |
2022 | Ijenali yeBiotechnology | 8.154 | Ukuchonga i-ST1 ityhila ukhetho olubandakanya ukubetha kwe-hitchhilogy morphology kunye nomxholo weoyile ngexesha lesoben
| Ukufowuna kwe-SNP |
2022 | Imida kwiSayensi yeSityalo | 6.623 | Imephu ye-genome-ngokubanzi yemephu ye-dunotypes ezinqabileyo kwimo yesazela.
| I-gwas |