I-RNA encinci Ukulandelelana-Illumina
Iimbonakalo
● Ukulungiswa kwethala leencwadi kubandakanya inyathelo lokukhetha ubungakanani
● Uhlalutyo lwe-Bioinformatic lugxile kuqikelelo lwe-miRNA kunye nezinto ekujoliswe kuzo
Iinkonzo eziluncedo
●Uhlalutyo olubanzi lwe-bioinformatics:Ukwenza ukuba kuchongwe zombini ii-miRNAs ezaziwayo kunye neenoveli, ukuchongwa kweethagethi ze-miRNAs, kunye nochazo olusebenzayo oluhambelanayo kunye nokutyebisa ngoovimba abaninzi (KEGG, GO)
●Ulawulo loMgangatho olungqongqo: Sisebenzisa iindawo zolawulo ezingundoqo kuzo zonke izigaba, ukusuka kwisampulu kunye nolungiselelo lwethala leencwadi ukuya kulandelelwano kunye ne-bioinformatics. Olu hlolo lucokisekileyo luqinisekisa ukuhanjiswa kweziphumo ezihlala zikumgangatho ophezulu.
●Inkxaso yePost-Sales: Ukuzinikela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi kunye neenyanga ezi-3 emva kokuthengiswa kwenkonzo. Ngeli xesha, sinikezela ngokulandelelana kweprojekthi, uncedo lokusombulula ingxaki, kunye neeseshoni ze-Q&A ukujongana nayo nayiphi na imibuzo enxulumene neziphumo.
●UbuGcisa obuBanzi: Ngerekhodi lokuvala ngempumelelo iiprojekthi ezininzi ze-sRNA ezigubungela iintlobo ezingaphezu kwama-300 kwiindawo ezahlukeneyo zophando, iqela lethu lizisa ubutyebi bamava kwiprojekthi nganye.
IiMfuno zeSampulu kunye nokuhanjiswa
| Ithala leencwadi | Iqonga | Idatha ecetyiswayo | Idatha ye-QC |
| Ubungakanani obukhethiweyo | Illumina SE50 | I-10M-20M ifundeka | Q30≥85% |
IiMfuno zeSampulu:
IiNucleotides:
| Conc.(ng/μl) | Imali (μg) | Ubunyulu | Ingqibelelo |
| ≥ 80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Unyino okanye akukho protein okanye ungcoliseko lweDNA olubonakaliswe kwijeli. | I-RIN≥6.0; 5.0≥28S/18S≥1.0; umphakamo osisiseko okanye ongekhoyo |
● Izityalo:
Ingcambu, Isiqu okanye iPetal: 450 mg
Igqabi okanye Imbewu: 300 mg
Isiqhamo: 1.2 g
● Isilwanyana:
Intliziyo okanye Amathumbu: 450 mg
Viscera okanye Brain: 240 mg
Izihlunu: 600 mg
Amathambo, Iinwele okanye Ulusu: 1.5g
● IiArthropods:
Izinambuzane: 9g
I-crustacea: 450 mg
● Igazi elipheleleyo: 2 iityhubhu
● Iiseli: 106 iiseli
● ISerum nePlasma:6 ml
Unikezelo lweSampuli eCetyisiweyo
Isikhongozeli: 2 ml centrifuge ityhubhu (Ifoyile yeTin ayikhuthazwa)
Isampuli yokuleyibhela: Iqela+liphindaphinda umz. A1, A2, A3; B1, B2, B3.
Ukuthunyelwa ngenqanawa:
1. Umkhenkce owomileyo: Iisampulu kufuneka zipakishwe kwiingxowa kwaye zingcwatywe kumkhenkce owomileyo.
2. Iityhubhu ze-RNAstable: Iisampulu ze-RNA zingomiswa kwi-tube ye-RNA yokuzinzisa (umzekelo, i-RNAstable®) kwaye ithunyelwe kwiqondo lokushisa.
Ukuhamba komsebenzi weNkonzo
Uyilo lovavanyo
Ukuhanjiswa kwesampuli
Ukukhutshwa kwe-RNA
Ukwakhiwa kwethala leencwadi
Ukulandelelanisa
Uhlalutyo lwedatha
Iinkonzo emva kokuthengisa
Bioinformatics
● Ulawulo lomgangatho wedatha ekrwada
● Ukuhlelwa kwe-sRNA
● Ukulungelelaniswa kwijenome yesalathiso
● Ukuchongwa kwe-miRNA eyaziwayo neyenoveli
● Uhlalutyo lwentetho ye-miRNA eyahlukileyo
● Inkcazo esebenzayo yeethagethi ze-miRNA
Ukuchongwa kwe-miRNA: isakhiwo kunye nobunzulu
Ukubonakaliswa okwahlukileyo kwe-miRNA - ukuhlanganisana kwe-hiearchical
Inkcazo esebenzayo yethagethi yee-miRNA ezichazwe ngokwahlukileyo
Phonononga ukuqhubela phambili kophando oluququzelelwa yi-BMKGene' sRNA iinkonzo zolandelelwano ngengqokelela egciniweyo yopapasho.
Chen, H. et al. (2023) 'Izifo zentsholongwane zivimbela i-saponin biosynthesis kunye ne-photosynthesis kwi-Panax notoginseng', i-Plant Physiology kunye ne-Biochemistry, i-203, iphe. 108038. doi: 10.1016/J.PLAPHY.2023.108038.
Li, H. et al. (2023) 'Isityalo se-FYVE esineprotheyini ye-FREE1 enxulumana namalungu e-microprocessor ukucinezela i-miRNA biogenesis', iingxelo ze-EMBO, 24(1). doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.
Yu, J. et al. (2023) 'I-MicroRNA Ame-Bantam-3p iLawula uPhuhliso lweLarval Pupal ngokuJonga i-Multiple Epidermal Growth Factor-like Domains 8 Gene (megf8) kwi-Honeybee, i-Apis mellifera', i-International Journal ye-Molecular Sciences, 24(6), p. . 5726. doi: 10.3390/IJMS24065726/S1.
UZhang, M. et al. (2018) 'Uhlalutyo oludityanisiweyo lwe-MiRNA kunye neGene eNxulunyaniswe noMgangatho weNyama Ibonisa ukuba i-Gga-MiR-140-5p ichaphazela i-Intramuscular Fat Deposition kwiiNkukhu', i-Cellular Physiology kunye ne-Biochemistry, i-46 (6), iphe. 2421-2433. doi: 10.1159/000489649.
