I-prokarytic ratenic
Iimbonakalo
● I-RNNA yesampulu ibandakanya ukupheliswa kweRRANA elandelwa ngulungiselelo lwethala leencwadi le-RNA.
● Uhlalutyo lwe-bioineformatic olusekwe kulungelelwaniso kwireferensi ye-genome
● Uhlalutyo lubandakanya ukubizwa nge-progs kunye nee-DEGS kodwa kwanokwakhiwa kwesakhiwo kunye nohlalutyo lwe-SRNA
Izibonelelo zeNkonzo
●Ulawulo oluqhelekileyo lomgangatho: Sisebenzisa amanqaku olawulo oluphambili kuwo onke amabakala, ukusuka kwisampulu kunye nokulungiswa kwelayibrari ukulandelelana kunye ne-bioincformatics. Ukuhlolisisa okunyanzelekileyo kuqinisekisa ukuhanjiswa kweziphumo ezisemgangathweni eziphezulu.
●Idatha ekhethekileyo ye-Strand: Ngenxa yethala leencwadi le-RNA ukuba lisalathisi, ukuvumela ukubekwa kokubhaliweyo okuchasene nemvakalelo.
●Uhlalutyo olupheleleyo lubekelwe i-prokarytic yothuthoI-biipeline ye-bioinformatic ibandakanya ukungahlalutyiwa kuphela kwenkcazo ye-fula kodwa ikwahle nohlalutyo lolwakhiwo lombhalo, kubandakanya nokuchongwa kwee-OpenONS, i-UTRS kunye nokukhuthaza. Ikwabandakanya uhlalutyo lwee-srnas, ezizezinyani kunye nokuxelwa kwesakhiwo esisesibini kunye neethagethi.
●Inkxaso yasemva kwentengiso: Ukuzinikela kwethu kugqitha ngaphaya kokugqitywa kweprojekthi kunye nexesha lenkonzo yenkonzo yasemva kwenkonzo emva kwenkonzo. Ngeli xesha, sinikezela ngeprojekthi yokulandela iprojekthi, uncedo lokucombulula iingxaki, kunye ne-Q & Iiseshoni zokujongana nayo nayiphi na imibuzo enxulumene neziphumo.
IiMfuno kunye nokuHanjiswa
| Ithala leencwadi | Isicwangciso sokulandelelana | Idatha eyacetyiswayo | Ulawulo lwemeko |
| I-RRNA iphelele ithala leencwadi | I-Mulmina Pe150 | I-1-2 GB | I-Q30≥85% |
Iifoto zeSampula:
| I-TRI. (G / μl) | Isixa (μg) | Ubunyulu | Ingqibelelo |
| ≥ 50 | ≥ 1 | I-OD260 / 280 = 1.8-2.0 I-OD260 / 230 = 1.0-2.5 I-protein okanye iprotein okanye ungcoliseko lwe-DNA oluboniswe kwi-Gel. | Rin≥6.5 |
Ukuhanjiswa kwesampulu
Isikhongozeli: 2 Ml Centrifuge Tube (i-tin foil ayikhuthazwanga)
I-A1 yeQela + umz. A1, A2, A3; B1, B2, B3.
Ukuhanjiswa:
I-1. I-ice-yowomileyo: Iisampulu kufuneka zipakishwe ezingxoweni kwaye zingcwatyelwe kwi-ice eyomileyo.
I-2. Ii-tubs ezinobungozi: Iisampulu ze-RNNNA zinokomiswa kwi-tube yokuzinzisa i-RNA (umzekelo: i-rnastast®) kwaye ithunyelwe kubushushu begumbi.
Ukuhamba kwenkonzo
Ukuhanjiswa kwesampula
Ulwakhiwo lwethala leencwadi
Ukulandelelana
Uhlalutyo lwedatha
Iinkonzo zentengiso emva
I-bioinformatic yokuhlalutya ukusebenza
Kubandakanya nohlalutyo olulandelayo:
● Ulawulo loMgangatho wedatha
● Ulungelelwaniso kwireferensi ye-genome
● Uvavanyo olusemgangathweni wethala lencwadi: Ukuqunjelwa kwe-RNA ukungahlekisi, faka ubungakanani kunye nokugcwala ukugcwala
● Ukunyanzeliswa kokunyanzelwa kwe-genes eqikelelweyo
● Uhlalutyo: Unxibelelwano kunye nohlalutyo lwecandelo eliphambili (PCA)
● Inkcazo eyahlukileyo ye-tem (i-DEGG)
● Ukuchasana nokuchazwa kunye nokuphucula ii-degs
● Uhlalutyo lwe-SRNA: Uqikelelo, isichazi, ekujoliso, kunye nenqanaba lokuxoxiswa kwenqanaba lesibini
● Uhlalutyo lwe-Shestrerript yohlalutyo: Ii-Open, ukuqala nokuphelisa izikhundla, ingingqi engapheliyo (i-UTS), i-PSP / NOLWAZI
Ukugcwala ukugcwala
Ukunyanzeliswa kwe-anyanisi ye-genes
Ukulungelelaniswa phakathi kweesampulu
Umahluko ubonakalise uhlalutyo lwe-genes (degs)
Uhlalutyo olusebenzayo
I-SRNA Inkcazo
Jonga i-veractions eququzeleleke yi-Bmkgene's Nanopore egcwele iinkonzo ezigcweleyo kule mpapasho ibonisiweyo.
IGuan, CP et al. .I-Pourlish Gornali ye-microbiology, 67 (2), iphe. 223. DOI: 10.21307 / PJM-2018-024.
