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DNBSEQ amathala eencwadi enziwe kwangaphambili
I-DNBSEQ, ephuhliswe yi-MGI, bubuchwepheshe be-NGS obutsha obukwazile ukuhla nangakumbi kwiindleko zolandelelwano kunye nokunyusa umthamo. Ukulungiswa kweelayibrari ze-DNBSEQ kubandakanya ukuhlukana kwe-DNA, ukulungiswa kwe-ssDNA, kunye nokukhulisa isangqa esijikelezayo ukufumana i-nanoballs ye-DNA (DNB). Ezi ke ziye zilayishwe kumphezulu oqinileyo kwaye emva koko zilandelelaniswe ngokudityaniswa kweProbe-Anchor Synthesis (cPAS). Itekhnoloji ye-DNBSEQ idibanisa iingenelo zokuba nesantya esisezantsi sokukhulisa impazamo kunye nokusebenzisa iipatheni zempazamo zoxinaniso oluphezulu kunye neenanoballs, okukhokelela kulandelelwano nge-throughput ephezulu kunye nokuchaneka.
Inkonzo yethu yolandelelwano yethala leencwadi eyenziwe kwangaphambili yenza ukuba abathengi balungiselele amathala eencwadi olandelelwano lwe-Illumina ukusuka kwimithombo eyahlukeneyo (i-mRNA, i-genome epheleleyo, i-amplicon, iilayibrari ze-10x, phakathi kwezinye), eziguqulelwa kwiilayibrari ze-MGI kwiilabhoratri zethu ukuze zilandelelaniswe kwi-DNBSEQ-T7, eyenza amanani aphezulu edatha ngeendleko eziphantsi.
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Illumina amathala eencwadi enziwe kwangaphambili
Itekhnoloji yolandelelwano ye-Illumina, esekwe kulandelelwano nge-Synthesis (SBS), yinto entsha ye-NGS eyamkelwe kwihlabathi jikelele, enoxanduva lokuvelisa ngaphezulu kwe-90% yedatha yolandelelwano lwehlabathi. Umthetho-siseko we-SBS ubandakanya umboniso we-fluorescent obhalwe izitermina ezibuyiselwa umva njengoko i-dNTP nganye yongezwa, kwaye emva koko yacandwa ukuvumela ukufakwa kwesiseko esilandelayo. Ngazo zone ii-dNTP ezine-terminator-bound bounded dNTP ezikhoyo kumjikelo ngamnye wolandelelwano, ukhuphiswano lwendalo lunciphisa ukubandakanywa kokubandakanya. Ubuchwephesha obuguquguqukayo buxhasa amathala eencwadi afundwe kunye kunye nesiphelo sesibini, silungiselela uluhlu lwezicelo ze-genomic. I-Illumina yokulandelanisa i-high-throughput i-high-throughput ibeka njengelitye lembombo kuphando lwe-genomics, ixhobisa izazinzulu ukuba ziveze ubucukubhede be-genomes ngeenkcukacha ezingenakulinganiswa kunye nokusebenza kakuhle.
Inkonzo yethu yolandelelwano yethala leencwadi eyenziwe kwangaphambili yenza ukuba abathengi bakwazi ukulungiselela amathala eencwadi alandelelanayo ukusuka kwimithombo eyahlukeneyo (i-mRNA, i-genome epheleleyo, i-amplicon, iilayibrari eziyi-10x, phakathi kwezinye). Emva koko, la mathala eencwadi anokuthunyelwa kumaziko ethu olandelelwano lolawulo lomgangatho kunye nokulandelelana kwiiplatifti ze-Illumina.
