Isityalo / isilwanyana sonke i-genome
Iimpawu zenkonzo
● Ukulungiselela kwelayibrari kunokuba ngumgangatho okanye i-pcr-free
● Ifumaneka kwicandelo eli-4 lokulandelelana: I-Iltimina Novaq, Mgi T7, Nanopore Promethion p48, okanye i-PACBIO Resoreio.
● Uhlalutyo lwe-bioinformatic lugxile ekufumaneni ii-hlukanisa: i-SNP, i-Indel, sv kunye ne-CNV
Izibonelelo zeNkonzo
●Iingcali ezibanzi zengcali kunye neerekhodi zokupapasha: Amava aqokelelweyo kwi-genome ukulandelelana okungaphezulu kwe-1000 kubangele ukuba amatyala angama-1000 apapashiweyo ngempembelelo yempembelelo engaphezulu kwe-5000.
●Uhlalutyo olubanzi lwe-bioinformaticsIquka ukubizwa kwezinto kunye nomsebenzi.
● Inkxaso yentengiso yasemva kwentengiso:Ukuzibophelela kwethu kugqitha ngaphaya kokugqitywa kweprojekthi kunye nexesha lenkonzo yenkonzo yasemva kwenkonzo emva kwenkonzo. Ngeli xesha, sinikezela ngeprojekthi yokulandela iprojekthi, uncedo lokucombulula iingxaki, kunye ne-Q & Iiseshoni zokujongana nayo nayiphi na imibuzo enxulumene neziphumo.
●Isichazi esibanzi: Sisebenzisa oovimba beenkcukacha ezininzi ukuba basebenzise i-genes ngendlela eyahlukileyo echongiweyo kwaye benze uhlalutyo lokuphucula okuthengisiweyo, ukubonelela ngokuqonda kwiiprojekthi ezininzi zophando.
Inkcazo yenkonzo
| Ii-hluko eziza kuchongwa | Isicwangciso sokulandelelana | Ubunzulu obucetyiswayo |
| I-SNP kunye ne-ITel | I-Ilnumina Novaseq Pe150 okanye i-MGI T7 | I-10x |
| I-SV kunye ne-CNV (ichanekile) | I-30x | |
| I-SV kunye ne-CNV (ichanekile) | I-Nanopore Pup D48 | I-20x |
| I-SNPS, i-Indels, sv kunye ne-CNV | I-Pacbio Revio | I-10x |
Iifoto zeSampula
| Izicubu okanye zikhutshiwe i-acid ye-nucleic | I-Illina / Mgi | Nanopore | I-pacbio
| ||
| I-viscera yesilwanyana | I-0.5-1 g | ≥ 3.5 g
| ≥ 3.5 g
| ||
| Isihlunu sesilwanyana | ≥ 5 g
| ≥ 5 g
| |||
| Igazi laseMammalian | I-1.5 ml | I-0.5 ml
| ≥ 5 ml
| ||
| Inkukhu / igazi lentlanzi | I-0.1 ml
| I-0.5 ml
| |||
| Isityalo- amagqabi amatsha | 1-2 g | ≥ 2 g
| ≥ 5 g
| ||
| Iiseli ezikhulisiweyo |
| ≥ 1x107
| ≥ 1x108
| ||
| Inambuzane ethambileyo / umntu ngamnye | I-0.5-1 g | ≥ 1 g
| ≥ 3 g
| ||
| Ikhutshiwe i-DNA
| Uxinzelelo: ≥ 1 Ng / μl Isixa: ≥ 30 ng Inqongophele okanye akukho hlazo okanye ungcoliseko
| Uxinzelelo Isixa
Od260 / 280
I-OD260 / 230
Inqongophele okanye akukho hlazo okanye ungcoliseko
| ≥ 40 Ng / μl I-4 μg / iselfowuni / isampulu
1.7-12
≥1.5 | Uxinzelelo Isixa
Od260 / 280
I-OD260 / 230
Inqongophele okanye akukho hlazo okanye ungcoliseko | ≥ 50 NG / μl I-10 μg / iselfowuni / isampulu
1.7-12
1.8-2.5 |
| Ukulungiswa kwethala leencwadi kwi-PCR-Free: I-oxalters i-40 nge / μl Isixa-mali kwi-500 NG | |||||
Ukuhamba kwenkonzo
Ukuhanjiswa kwesampula
Ukukhutshwa kwe-DNA
Ulwakhiwo lwethala leencwadi
Ukulandelelana
Uhlalutyo lwedatha
Ukuhanjiswa kwedatha
Kubandakanya nohlalutyo olulandelayo:
- Ulawulo loMgangatho wedatha
- Iinkcukacha-manani zolungelelwaniso lwesalathiso se-genome
- Isazisi esahlukileyo: i-SNP, i-Indel, sv kunye ne-CNV
- I-ANGEAL INCONITIONALS
Iinkcukacha-manani zolungelelwaniso kwisalathiso se-genome-ukugcwala kobunzulu
Ukutsala umnxeba kwiisampulu ezininzi
Ukuchongwa kwe-Indl-Statistics yobude be-Indel kummandla we-CDS kunye nengingqi ebanzi ye-genome
Ukusasazwa okwahlukileyo kwi-genome-circos yento
Ukuchasana nokuchasana kwe-genes nge-quiring echongiweyo-kwi-edigy yegewu
Chai, Q. ET Al. . 433. Doi: 10.1111 / PBI.13965.
Cheng, H. ET Al. . Doi: 10.1111 / PBI.14018.
Li, A. ET Al. . Doi: 10.1038 / S42003-021-02823-6-6.
Zeng, t. et al. . Doi: 10.1038 / S42003-022-03907-7.
