I-non-rerever esekwe kwi-MRNA ye-SHANA
Iimbonakalo
● UKUFUNYANWA KWE-POLY MRNA ngaphambi kokulungiswa kwethala leencwadi
● Ngaphandle kwayo nayiphi na ireferensi ye-genome: isekwe kwiNdibano yokushicilela, ivelisa uluhlu lwee-Unicts ezichazwe kwi-databa ye-daties ezininzi (i-NR, i-Swiss, i-Kog, i-Eggnog, hamba, uye, uKegg)
● Uhlalutyo olubanzi lwe-bioineformatic lwentetho yesimumu kunye nolwakhiwo lobhalo
Izibonelelo zeNkonzo
●Ubuchule obubanziIngxelo yerekhodi yokulungisa iisampulu ezingama-600,000 kwi-Bmkgene, iindidi ezahlukeneyo ezahlukeneyo ezinjengenkcubeko, izicubu, kunye nolwelo lomzimba, iqela lethu lizisa ubutyebi bamava kuzo zonke iiprojekthi. Sivale ngempumelelo i-100,000 MRNA-SEQ kwiiprojekthi ezahlukeneyo zophando.
●Ulawulo oluqhelekileyo lomgangatho: Sisebenzisa amanqaku olawulo oluphambili kuwo onke amabakala, ukusuka kwisampulu kunye nokulungiswa kwelayibrari ukulandelelana kunye ne-bioincformatics. Ukuhlolisisa okunyanzelekileyo kuqinisekisa ukuhanjiswa kweziphumo ezisemgangathweni eziphezulu.
● Inkcazo ebanzi: Sisebenzisa i-databas eninzi ukuba ichaze ngokwahlukileyo kwe-genes (i-degs) kwaye yenze uhlalutyo oluhambelana nokuhambelana, ukubonelela ngenkqubo yokuphendula i-selcriptom.
●Inkxaso yasemva kwentengiso: Ukuzinikela kwethu kugqitha ngaphaya kokugqitywa kweprojekthi kunye nexesha lenkonzo yenkonzo yasemva kwenkonzo emva kwenkonzo. Ngeli xesha, sinikezela ngeprojekthi yokulandela iprojekthi, uncedo lokucombulula iingxaki, kunye ne-Q & Iiseshoni zokujongana nayo nayiphi na imibuzo enxulumene neziphumo.
IiMfuno kunye nokuHanjiswa
| Ithala leencwadi | Isicwangciso sokulandelelana | Idatha eyacetyiswayo | Ulawulo lwemeko |
| I-Poly A ityetyisiwe | I-Mulmina Pe150 I-DNBseq-t7 | I-6-10 GB | I-Q30≥85% |
Iifoto zeSampula:
I-NuCuotides:
| I-TRI. (G / μl) | Isixa (μg) | Ubunyulu | Ingqibelelo |
| ≥ 10 | ≥ 0.2 | I-OD260 / 280 = 1.7-2.5 I-OD260 / 230 = 0.5-2.5 I-protein okanye iprotein okanye ungcoliseko lwe-DNA oluboniswe kwi-Gel. | Kwizityalo: rin≥4.0; Kwizilwanyana: rin≥4.5; 5.0≥28s / 18s≥1.0; Inqunu okanye akukho kukuphakama kwesiseko |
● Izityalo:
Ingcambu, i-stem okanye petal: 450 mg
Igqabi okanye imbewu: 300 mg
Iziqhamo: 1.2 g
● Isilwanyana:
Intliziyo okanye iesine: 300 mg
I-Vicera okanye ingqondo: I-240 mg
Imisipha: 450 mg
Amathambo, iinwele okanye ulusu: 1g
● I-Arthropods:
Izinambuzane: I-6G
I-crustacea: 300 mg
● Igazi liphela: I-1 ityhubhu
● Iiseli: 106 iiseli
Ukuhanjiswa kwesampulu
Isikhongozeli: 2 Ml Centrifuge Tube (i-tin foil ayikhuthazwanga)
I-A1 yeQela + umz. A1, A2, A3; B1, B2, B3.
Ukuhanjiswa:
I-1. I-ice-yowomileyo: Iisampulu kufuneka zipakishwe ezingxoweni kwaye zingcwatyelwe kwi-ice eyomileyo.
I-2. Ii-tubs ezinobungozi: Iisampulu ze-RNNNA zinokomiswa kwi-tube yokuzinzisa i-RNA (umzekelo: i-rnastast®) kwaye ithunyelwe kubushushu begumbi.
Ukuhamba kwenkonzo
Uyilo lovavanyo
Ukuhanjiswa kwesampula
Ukukhutshwa kwe-RNA
Ulwakhiwo lwethala leencwadi
Ukulandelelana
Uhlalutyo lwedatha
Iinkonzo zentengiso emva
I-Bioinformatics
INdibano yeNdibano yokuSebenza kunye noKhetho lweNgingqi
I-Univene Inkcazo
Isampulu yokulungelelanisa kunye novavanyo lweendlela zebhayoloji
Ngokwahluka kwangaphandle
Ukuchasana nokuchasana kwee-Degs
Ukuphuculwa kwe-Degs
Jonga i-veractions eququzeleleke yi-Bmkgene's Eukrnatic NGS MRNA YENKONZO YOKUFUNDA NGOKUFANELEKILEYO.
Shen, F. ET AL. . DoI: 10.1016 / J.YGeno.20.01.026.
Zhang, c. et al. . 212763. Doi: 10.3389 / FPLS.2016.01425 / i-bibtex.
Zhu, c. et al. . Doi: 10.1371 / Ijenali.pone.0171966.
Zou, l. et al. . DoI: 10.1186 / S12770-021-032744-1 / Amanani / 9.
