Indaleko ivele
Pnas
Imvelaphi yendaleko kunye nendlu yendlu ye-Goldfish (Caurius Auratus)
I-Pacbio | I-Mulina | Imephu ye-biodano ye-genome | Hi-c genome indibano | Imephu yemfuza | I-GWAS | I-rna-seq
Amagqabantshintshi
1. IGenome yaseGenome ihlaziywe ngendibano yendibano ephezulu, i-adhoring 95.75% ye-stadochrosomes kwi-50 pseudo yoko (Scaffold N50 = 31.84 mb). I-subsomes ezimbini zenziwa.
2. Imimandla yezenyama ye-Teept ye-Sweept ngexesha lendlu ichongiwe kwidatha yabantu, ityhila ngaphezulu kwe-390 yeemfumba ezinokunxulunyaniswa neempawu zendlu.
3.gwas kwi-dorsal fin kwi-Forenfish efudukayo ityhilwe i-378 i-geneuatate yemfuza enxulumene nokunxulunyaniswa ngokufanelekileyo. I-Tyrosine-Protein Proterin Prouper ikhutshiwe njengomgqatswa we-causal gene
Imvelaphi
I-Goldfish (Caurius Auratus) yenye yezona ntlanzi zibalulekileyo zefama, ezazichongwe kwi-carcian carp kwi-China yakudala. Bagqabazekile nguCharles Darwin 'ngokudlula ngaphezulu kweyantlukwano engapheliyo yombala, sidibana nolona hlengahlengiso lubalaseleyo lwesakhiwo ". Iinkalo ezihlukileyo kakhulu kunye nembali ende ye-imeyile yendlu kunye nokuzala zenza inkqubo yemodeli yemfuza efanelekileyo yeFilisi yeFizi lentlanzi kunye nendaleko.
Impumelelo
I-Goldfish Genome
JUhlalutyo lwe-OIT lwe-pacbio kunye ne-Inlina yokuLungelelanisa iNdawo yokuLawula ivelisa iNdibano yokuqala ye-1.657 g (inkampani ye) (474 KB). Imephu ye-bionano eyenziwa kwaye yalungisa indibano i-1.73 GB ngobukhulu (ubuqili-mali eqikelelweyo: 1.8 GB). INdibano yeHi-C iqhubeke iphucule i-scaffold nN isuka kwi-606 kb ukuya kwi-31.84 MB kwaye iphumelele i-95.75% (1.65 ye-GB) ye-adhoring (1.65 ye-GB) ye-adhoring (1.65 ye-GB) ye-adhoring (i-1.65 GB) i-Adperiering kunye ne-Adhoring. I-genome iquka i-56,251 ye-genes kunye ne-10,098 ixesha elide elingemva kokubhaliweyo. Ngaphezu koko, imimandla emiselweyo engama-38 ixelwe kwangaphambili kwiiChromosome ezingama-50.
I-Fig.1 yeGolide yeFoily Genome
TUya kucaca iiseti ze-subthamnomes kwi-50 ye-chrisosomes ekhokelele kumsitho wamandulo we-hybrida. Iseti ye-chromosome enenani eliphezulu lokufunda phakathi kwegolide ehambelana neGoldish noBarbinae bachazwa njenge (chra01 ~ A25), okt i-subgenome iqhelekileyo eBarbinome B (Chrb01 ~ B25).
Indlu kunye nokukhetha i-sweeps
AIyonke ye-16 yohlobo lwe-Deedy Crub Cerphus kunye ne-185 ye-185 ye-phyfish eyahlukileyo yokufowunela ngokulinganayo kubunzulu be-12x, malunga ne-4.5x yedatha. Ulwakhiwo lwe-phylogenetic ukwakha kunye nohlalutyo lwe-PCA luqinisekisile ulwalamano olusondeleyo phakathi kweGoldfish kunye neGolide yaseGoldfish kunenye iSbyfish, enamacala amabini.
LD Uhlalutyo lokulonda malunga ne-subpopulations ezine exhasa ubukho bebhotile yemfuza yemfuza yemveli ngexesha lokukhetha indlu kunye nokukhetha okomeleleyo kwi-Golfish. Ukonyusa iyantlukwano yemfuzo Iingingqi ezingama-50 zokukhetha i-Shemicgesic ezigubungela i-25.2 MB kunye ne-946 iintlobo zemfuza ezichongiweyo kwiDatha (33 Goldfish kunye ne-16 Crubian). Ukwandiswa kohlalutyo ku-2010, 393 imfuza ebonisile imimandla yokutsha okukhethekileyo. Ezi ntlobo zemfuza zifunyenwe zeyantlukwano eziphantsi, ezinokuba negalelo kwi-phenotypes ezinxulumene ne-FineredIts eziphambili kwi-Golfish.
I-Fig.3 ye-Genome-ngokubanzi uhlalutyo olunxulumene nohlalutyo
I-GWAS kwiFolfish yeFolfish
DI-Orsal Fin yeyona nto iphambili eyahlula i-wen ye-Wincyfish evela kwi-Egg Golfish. I-GWAS ye-Dorsel Fine kwi-96 ye-Winfish kunye ne-Golgfish engama-378 ivele i-378 yeegesi ezisasazekayo kwi-13 ye-chromosomes ezisasazwayo kunye nokusasazwa kwezi ntlobo ziphakathi kwemfuza kuzo. Uhlalutyo olusebenzayo kwezi ntlobo zebhayilohu zichaze iinkqubo zebhayiloji zibandakanya "ukufunyanwa komgangatho weseli", "Transrbrane Couth", "njl.
Umzobo
II-GWAS yeempawu ezinxulumene nezixhobo ezibonakalayo, kwafunyanwa i-peak enye eyomeleleyo. I-Geeding encoding I-Tyrosine-Proupine yeTypy Protein Kinase i-Kinase i-Ninase ichongelwe kwenye yemimandla emigqatso.
Fig.5 Gwas yempawu ezinxulumene nezixhobo ezibonakalayo
Ireferensi
Chen d et al. Imvelaphi yendaleko kunye nendlu yendlu ye-Goldfish (Caurius Auratus). I-PNA (2020)
Iindaba Ijolise ekwenziseni amatyala anempumelelo anempumelelo kunye neetekhnoloji ze-biomarker, ifake impumelelo yesayensi yenzululwazi kunye neendlela ezidumileyo ezisetyenziswayo ngexesha lophononongo.
IXESHA LOKUQALA: UJan-04 ukuya ku-522