I-Nanopore-based Full-length-length Transcriptome Sequencing
Ulandelelwano lwe-Nanopore luzahlula kwezinye iiplatifomu zokulandelelana, kuba i-nucleotides ifundwa ngokuthe ngqo ngaphandle kwe-DNA synthesis kwaye ivelise ukufundwa okude kumashumi e-kilobases. Oku kuxhobisa ukufundwa ngokuthe ngqo kwemibhalo yobude obupheleleyo kunye nokujongana nemingeni kwizifundo ze-transcriptomic.
√ Ulandelelwano oluthile olukhethekileyo
√ Ufundo olupheleleyo lwe-cDNA ukuya kwizifundo zobume bemfuza
√ Iinkcukacha ezimbalwa ezifunekayo ukuquka inani elifanayo lemibhalo ekhutshelweyo
√ Ukuchongwa kwee-isoforms ezininzi kwijeni ngalinye
√ Ubalo lwenkcazo kumgangatho we-isoform
Umbhobho weBioinformatics oqhelekileyo
Amatyala aMpumelelo amva nje ngeBiomarker Technologies
1. Ucazululo oluthelekisayo lwe-transcriptomes yobude obugcweleyo lutyhila i-Gnetum luofuense stem development dynamics.
Ijenali: Imida kwiGenetics
Ipapashwe: Matshi 2021
Amagama angundoqo: Minion | Enye indlela yokudibanisa | APA | INCRNA | Imibhalo ebhalwe ngokwahlukileyo
Funda isicatshulwa esipheleleyo
2. Ukunyakaza okungafaniyo kwee-RNA ezincinci ukusuka kwiingcambu ukuya kwiingcambu kwi-interspecific heterografts
Ijenali: Izityalo zendalo
Ipapashiwe: ngoJanuwari 2021
Amagama angundoqo: Nanopore | Ilumina | ubhaqo lwemRNA egcweleyo ephathwayo
3. Uhlalutyo olupheleleyo lwe-transcriptome yobude beengcambu ze-asparagus lubonisa indlela yemolekyuli yokunyamezela ityuwa eyenziwa yi-arbuscular mycorrhizal fungi.
Ijenali: Inzululwazi yendalo esingqongileyo kunye neyoMfuniselo
Ipapashiwe: ngoJanuwari 2021
Amagama angundoqo: Minion | Imibhalo ebhalwe ngokwahlukileyo | Imiba yoshicilelo | Enye indlela yokudibanisa
4. Utshintsho lweMpawu yePhysiological kunye neTranscriptome yoBubude obuPheleleyo beRose (iRosa chinensis) Iingcambu kunye namagqabi ekuphenduleni kuxinzelelo lwembalela.
Ijenali: iPhysiology yeZityalo kunye neSeli
Ipapashiwe: Okthobha 2020
Amagama angundoqo: PromethION | Imibhalo ebhalwe ngokwahlukileyo | Uqikelelo olusebenzayo | I-isoforms edibeneyo | I-TF kunye ne-lncRNA
5. Uhlalutyo kunye nothelekiso olubanzi lwe-PacBio kunye ne-nanopore-based based RNA sequencing ye-Arabidopsis transcriptome
Ijenali: Indlela yokuTyalwa
Ipapashwe: Epreli 2020
Amagama angundoqo: Igridi | PromethION | I-PacBio Sequel | Illumina NovaSeq | Ulungelelwaniso lweGenome | Ukuchongwa okushicilelweyo | Enye indlela yokudibanisa | SSR | LncRNA | Ubungakanani be-isoform
Iindaba neembalasane ijolise ekwabelaneni ngamatyala aphumeleleyo amva nje kunye neBiomarker Technologies, ukubamba impumelelo yesayensi enoveli kunye nobuchule obubalaseleyo obusetyenzisiweyo ngexesha lokufunda.
Ixesha lokuposa: Jan-08-2022