I-rology ye-necology
Ukuqina kwezolimo kunciphisa ubunzima be-microbial kunye nentabalala ye-keystone teksi kwiingcambu
Ukulandelelana okungapheliyo kwe-Amptican I-pacbio hifi
Amagqabantshintshi
AUkuqina kwabantu abadala kuyenzeka ngakumbi ngenxa yemo engqongqo yendalo kubandakanya ukusebenza kwesondlo, ukusetyenziswa kwesondlo se-goundwater, ukonakaliswa komhlaba kubandakanya okungaphezulu kokunciphisa ingozi. Uluntu oluhle ludlala indima engenakuphikiswa kwimveliso kunye nokuzinza kwe-Agroecosysysysysystem. Nangona kunjalo, kuyinto engacacanga ukuba iindlela ezahlukeneyo zokulima zichaphazela ngayo iingcambu ze microbiota.
Uyilo lovavanyo
Iimvavanyo
SIisampulu kunye neengcambu zengca (i-DNA) zazivela kwiindawo zengqolowa ukusuka kwi-60 yefama (i-20 nganye)
Gukungcungcutheka: 1. Indibano (kunye ne-tillage); 2. Ingqungquthela (i-no-tillage); I-3. Ifama ye-organic
SIsicwangciso esiliqili: ubude be-Amptican
PI-Rimrs: I-A1F-Iso-Iso-Iso (ukujolisa kuyo yonke imimandla yayo ~ 630 BP)
SIPlamfocing eDityanisiweyo: I-Pacbio Rs II
Uhlalutyo lwe-bioinformatic
Iziphumo
On I-Avatint ye-Otus engama-357 ichongiwe kwindawo nganye kunye ne-837 ye-otus yazo zonke iindawo ezingama-60. Ukwahlukahluka kweengcambu zoluntu akukubonisi mahluko phakathi kwenkqubo ezintathu zokulima. Nangona kunjalo, amaqela amathathu ahlukileyo asekwe kuhlalutyo lwe-Beta yohlalutyo, ebonisa iziphumo ezomeleleyo zenkqubo yokulima kwingcambu yoluntu.
Umzobo 1. Isalathiso se-ALLANNIty
TI-en ye-ceystone icacisiwe ngokusekwe kunothungelwano olupheleleyo lweendawo ezintathu zokuntywila kwimigangatho emithathu yokulima: Iindawo ezi-10 eziphezulu ezinesidanga esiphezulu, eyona ndawo iphantsi kakhulu kwaye iphantsi kakhulu kwaye iphantsi kakhulu. Okwesixhenxe kubo baba yimiyalelo ye-Mycorhyal.
Umzobo 2. Inethiwekhi iyonke kwiingcambu zoluntu lweenkqubo ezintathu zokulima
FI-Network-Inkqubo eyongamisiweyo ibonise ukunxibelelana okuphezulu kwinethiwekhi ye-orgactic enemiphetho kabini kunye neendawo ezixineneyo kunenethiwekhi ngaphandle kwenethiwekhi kude kube ngenye inethiwekhi. Ngaphezu koko, inethiwekhi ye-organic yenethiwekhi inomxholo omkhulu we-keystone Tearba (idayimani) xa kuthelekiswa nokuphumla, okuxhasa ukuntywila kwayo kunye nokunxibelelana
Umzobo 3. Inkqubo yokulima-iNethiwekhi yeNtloko zeNtloko
AUmanyano olubi olungalunganga phakathi kobunzulu bezolimo kunye neengcambu zenethiwekhi uqhagamshelo lwenethiwekhi luyaqwalaselwa. Uhlalutyo lwehlathi elingenamkhethe luveze abaqhubi abaziintloko zeKyystone kwiTeksi: I-phosphorus yomhlaba, uxinzelelo lwe-bulk, ubuninzi, i-PH kunye ne-MycorrhIzal.
Umzobo 4. Izolimo Ubunzulu kunye nokudityaniswa kwenethiwekhi kwiinkqubo ezintathu zokulima (a kunye b); Uhlalutyo lwehlathi elingenamkhethe (c) kunye nolwalamano phakathi kobunzulu bezolimo kunye ne-AMF boclonition (D)
Itekhnoloji
Ukulandelelana okungapheliyo
AS "Ukulandelelana kwesinye isizukulwana esizayo" ukuya kwinqanaba, ukunqongophala kwimimandla ekujoliswe kuyo kunye neengxaki kwiNdibano yeNovo ye-de novo. I-Pacific biocie (i-pacbio) yandisa ngempumelelo ukufunda ngokulandelelana kwamashumi eekhilontase, evumela ukuba sifumane ubude be-16s RRNA (i-1,500 BP) kwi-bacteria okanye i-1,500 BP-2,000 BP Imimandla (400 BP-900 BP) e-Eukarartotics. Imbono eyandileyo yentsimi yemvelo iphucule kakhulu isisombululo seendidi zokuchazwa kunye nemfuza esebenzayo. Umcimbi ochaphazelekayo ochaphazelekayo kwi-occharacy ochanekileyo sele isonjululwe yi-PACBBIO CCS, evelisa i-hifi ifunde ngokuchaneka okungaphezu kwe-99% ukuchaneka okufundileyo.
Intsebenzo kwi-otu
TIzibonelelo zokuzingela zombini ezi zinto zifundwayo zombini zifundwayo kunye nokuchaneka kwenkcazo kungakwaziswa ngokumangalisayo kunye nokufezekisa isisombululo se "nqanaba" kwizisombululo ze-micronial.
Ireferensi
Bangerjee, Samiran, et al. "Ukuqina kwezolimo kunciphisa intsokotha ye-microbial kunye nentabalala ye-keystone rista kwiingcambu." Ijenali ye-Isme (2019).
Itekhnoloji kunye neLiqalo Ijolise ekwabelaneni ngokusebenzisana ne-texilogies yendlela eyahlukileyo yokufaka i-resaight kwi-resaight kunye neengcinga eziqaqambileyo kwilondolozo lovavanyo kunye nokumbiwa kwedatha.
IXESHA LOKUQALA: UJan-08-2022