I-Nanopore Ubude obupheleleyo be-transcriptome

I-Nanopore transcriptome sequencing yindlela enamandla yokulandelelanisa i-cDNAs yobude obupheleleyo, ukuchonga ngokuchanekileyo kunye nokulinganisa i-isoforms ye-transcript. I-BMKCloud Nanopore Ubude obugcweleyo be-Transcriptome Pipeline yenzelwe ukuhlalutya idatha ye-RNA-Seq eveliswe kwiqonga le-Nanopore ngokubhekiselele kwi-genome ye-reference reference, enikezela uhlalutyo olunobunjani kunye nobungakanani kwinqanaba le-gene kunye ne-transcript. Emva kokulawulwa komgangatho, ulandelelwano olupheleleyo lwe-non-chimeric (FLNC) olupheleleyo lufunyenwe kwaye ulandelelwano lokuvumelana lufakwe kwimephu ye-reference genome ukususa imibhalo engafunekiyo. Ukusuka kule seti yokubhaliweyo, inkcazo ibalwa kwaye ibonakaliswe ngokwahlukileyo kwiijeni kunye nemibhalo ebhaliweyo ichongiwe kwaye ichazwe ngokusebenzayo. Umbhobho ukwabandakanya uhlalutyo olulolunye lwe-polyadenylation (APA), uhlalutyo olulolunye, uhlalutyo olulula lokulandelelana (SSR), uqikelelo lwe-lncRNA kunye neethagethi ezihambelanayo, uqikelelo lolandelelwano lwekhowudi (CDS), uhlalutyo losapho lwemfuza, uhlalutyo lwento ebhaliweyo, ukubikezelwa kofuzo lwenoveli. kunye nenkcazo esebenzayo yemibhalo ebhaliweyo.