mRNA-seq (NGS) – De novo

Ulandelelwano lwe-mRNA luvumela ukuchazwa kwayo yonke imibhalo ye-mRNA kwiiseli phantsi kweemeko ezithile kwaye bubuchwephesha obusetyenziswa ngokubanzi kwiindawo ezahlukeneyo zophando. Umbhobho we-BMKCloud De novo mRNA-seq uyilelwe ukuhlalutya iilayibrari ezicwangcisiweyo zepoly-A xa kungekho genome yesalathiso ekhoyo. Umbhobho uqala ngolawulo lomgangatho, ulandelwe ngude novoindibano yokubhala kunye nokukhethwa kwesethi ye-unigene. Uhlalutyo lwesakhiwo se-unigene luqikelela ukulandelelana kwekhowudi (CDS) kunye nokuphindaphinda okulandelelanayo okulula (SSR). Emva koko, uhlalutyo lokubonisa umahluko lufumana iijene ezichazwe ngokwahlukileyo (DEGs) phakathi kweemeko ezivavanyiweyo, zilandelwa sisichasiselo esisebenzayo kunye nokutyetyiswa kwee-DEGs ukukhupha i-biological insights.