I-Hi-C esekelwe kwi-Chromatin Interaction
Iimpawu zeNkonzo
● Ukulandelelanisa kwi-Illumina NovaSeq nge-PE150.
● Inkonzo ifuna iisampulu zethishu, endaweni yee-nucleic acids ezikhutshiweyo, ukuze zidibanise i-formaldehyde kwaye zilondoloze ukudibana kwe-DNA-protein.
● Uvavanyo lwe-Hi-C lubandakanya uthintelo kunye nokulungiswa kokuphela kweziphelo ezincangathi nge-biotin, kulandelwa kukujikeleziswa kweziphelo ezibuthuntu ezibe neziphumo ngelixa ugcina unxibelelwano. I-DNA ke itsalwa phantsi ngamaso e-streptavidin kwaye ihlanjululwe ukulungiselela ukulungiswa kwethala leencwadi elilandelayo.
Iinkonzo eziluncedo
●Uyilo lwe-Enzyme yoMthintelo oluLungileyo: ukuqinisekisa ukusebenza kakuhle kwe-Hi-C kwiintlobo ezahlukeneyo ukuya kuthi ga kwi-93% yezibini ezisebenzayo zokusebenzisana.
●UbuGcisa obuBanzi kunye neeRekhodi zoPapasho:I-BMKGene inamava amakhulu nge>2000 yeeprojekthi zolandelelwano lwe-Hi-C ukusuka kwiindidi ezingama-800 ezahlukeneyo kunye namalungelo awodwa abenzi. Ngaphezulu kwe-100 leemeko ezipapashiweyo ezinempembelelo eyongezelelweyo engaphezulu kwama-900.
●Iqela leBioinformatics elinezakhono eziphezulu:ngamalungelo awodwa omenzi wechiza angaphakathi kunye namalungelo oshicilelo esoftware yovavanyo lwe-Hi-C kunye nohlalutyo lwedatha kunye nesoftware yedatha yokubona ephuhlisiweyo.
●Inkxaso yasemva kwentengiso:Ukuzinikela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi kunye neenyanga ezi-3 emva kokuthengiswa kwenkonzo. Ngeli xesha, sinikezela ngokulandelelana kweprojekthi, uncedo lokusombulula ingxaki, kunye neeseshoni ze-Q&A ukujongana nayo nayiphi na imibuzo enxulumene neziphumo.
●Isichasiselo esiBanzi: sisebenzisa ugcino-lwazi oluninzi ukuze sichaze ngokusebenzayo iijini ezineenguqu ezichongiweyo kwaye senze uhlalutyo oluhambelanayo lokutyebisa, ukubonelela ngemibono kwiiprojekthi zophando ezininzi.
IiNgcaciso zeNkonzo
| Ithala leencwadi | Isicwangciso solandelelwano | Imveliso yedatha ecetyiswayo | Hi-C Signal Resolution |
| Hi-C ilayibrari | Illumina PE150 | I-Chromatin loop: 150x TAD: 50x | I-Chromatin Loop: 10Kb TAD: 40Kb |
IiMfuno zeNkonzo
| Uhlobo lwesampuli | Imali efunekayo |
| Izicubu zezilwanyana | ≥2g |
| IGazi Elipheleleyo | ≥2mL |
| Iifungus | ≥1g |
| Isityalo- izicubu ezincinci | 1g/aliquot, 2-4 aliquotes ezicetyiswayo |
| Iiseli Cultured | ≥1x107 |
Ibandakanya uHlahlelo Olulandelayo:
● Idatha ekrwada ye-QC;
● Ukwenza iMaphu kunye nelayibrari ye-Hi-C ye-QC: izibini zonxibelelwano ezisebenzayo kunye ne-Interaction Decay Exponents (IDEs);
● IProfayili yoKusebenzisana ngokubanzi kweGenome: uhlalutyo lwe-cis/trans kunye nemephu yokunxibelelana ye-Hi-C;
● Uhlalutyo losasazo lwekhompatimenti ye-A/B;
● Ukuchongwa kwee-TAD kunye neelophu zechromatin;
● Uhlalutyo olwahlukileyo kwizinto ze-3D zesakhiwo se-chromatin phakathi kweesampuli kunye nenkcazo esebenzayo ehambelanayo yeejeni ezinxulumene nazo.
Cis kunye nokuhanjiswa komlinganiselo
Imephu yobushushu yonxibelelwano lwechromosomal phakathi kweesampulu
Ukusasazwa ngokubanzi kweGenome kwamacandelo e-A/B
Ukusabalalisa ngokubanzi kwe-genome ye-chromatin loops
Ukubonwa kwee-TADs
Phonononga ukuqhubela phambili kophando oluququzelelwa yi-BMKGene ye-Hi-C yeenkonzo zolandelelwano ngengqokelela egciniweyo yopapasho.
Meng, T. et al. (2021) 'Uhlalutyo oludibeneyo oludibeneyo lwe-omics luchonga i-CA2 njengento ekujoliswe kuyo inoveli ye-chordoma',I-Neuro-Oncology, 23(10), iphepha 1709-1722. doi: 10.1093/NEUONC/NOAB156.
Xu, L. et al. (2021) 'Ukulungelelaniswa kwe-3D kunye nokulungiswa ngokutsha kwe-genome kunika ulwazi kwi-pathogenesis ye-NAFLD ngokudibanisa kwe-Hi-C, i-Nanopore, kunye ne-RNA',Acta Pharmaceutica Sinica B, 11(10), iphepha 3150-3164. doi: 10.1016/J.APSB.2021.03.022.
