Uhlalutyo lwe-Genome-wide Association
Ukuhamba komsebenzi
Iinkonzo eziluncedo
●UbuGcisa obuBanzi kunye neerekhodi zopapasho: ngamava aqokelelweyo kwi-GWAS, i-BMKGene igqibe amakhulu eeprojekthi zeentlobo kuphando lwabantu be-GWAS, incedise abaphandi ukuba bapapashe amanqaku angaphezu kwe-100, kwaye impembelelo eyongezelelweyo ifikelele kwi-500.
● Uhlalutyo olubanzi lwe-bioinformatics: Ukuhamba komsebenzi kubandakanya uhlalutyo lwe-SNP-trait association, ukuhambisa isethi yejene lomgqatswa kunye nenkcazo ehambelanayo yokusebenza.
●Iqela le-bioinformatics elinezakhono eziphezulu kunye nomjikelo omfutshane wohlalutyo: ngamava amnandi kuhlalutyo oluphambili lwe-genomics, iqela le-BMKGene linikezela ngohlahlelo olubanzi kunye nexesha elikhawulezayo lokuguqula.
●Inkxaso yasemva kwentengiso:Ukuzinikela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi kunye neenyanga ezi-3 emva kokuthengiswa kwenkonzo. Ngeli xesha, sinikezela ngokulandelelana kweprojekthi, uncedo lokusombulula ingxaki, kunye neeseshoni ze-Q&A ukujongana nayo nayiphi na imibuzo enxulumene neziphumo.
Iinkcazo zenkonzo kunye neemfuno
| Uhlobo lolandelelwano | Umlinganiselo wabemi ocetyiswayo | Isicwangciso sokulandelelana | Iimfuno zeNucleotide |
| Ulandelelwano lweGenome epheleleyo | 200 iisampulu | 10x | Ugxininiso: ≥ 1 ng/ µL Imali iyonke≥ 30ng Umda okanye akukho ukuthotywa okanye ukosuleleka |
| Iqhekeza leNdawo Ekhethekileyo-Eyandisiweyo (SLAF) | Ubunzulu bethegi: 10x Inani leethegi: < 400 Mb: I-WGS iyacetyiswa < 1Gb: 100K iithegi 1Gb > 2Gb: 300K iithegi Ubuninzi bethegi ezingama-500k | Ugxininiso ≥ 5 ng/µL Imali iyonke ≥ 80 ng Nanodrop OD260/280=1.6-2.5 Ijeli yeAgarose: akukho okanye ukuthotywa okulinganiselweyo okanye ungcoliseko
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Ukukhethwa kwezinto
Iindidi ezahlukeneyo, i-subspecies, i-landraces / i-genebanks / iintsapho ezixutywe / izixhobo zasendle
Iintlobo ezahlukeneyo, i-subspecies, i-landraces
Usapho lwesiqingatha sosapho / usapho olugcweleyo / izixhobo zasendle
Ukuhamba komsebenzi weNkonzo
Uyilo lovavanyo
Ukuhanjiswa kwesampuli
Ukukhutshwa kwe-RNA
Ukwakhiwa kwethala leencwadi
Ukulandelelanisa
Uhlalutyo lwedatha
Iinkonzo emva kokuthengisa
Ibandakanya uhlalutyo olulandelayo:
- Uhlalutyo lwe-Genome-wide association: LM, LMM, EMMAX, imodeli ye-FASTLMM
- Inkcazo esebenzayo yofuzo lomviwa
Uhlalutyo lwe-SNP-trait association - iploti yaseManhattan
Uhlalutyo lwe-SNP-trait association - icebo le-QQ
Phonononga inkqubela phambili eququzelelwa ziinkonzo ze-BMKGene's de GWAS ngengqokelela ehleliweyo yopapasho:
Lv, L. et al. (2023) 'Ukuqonda kwisiseko sofuzo sokunyamezela i-ammonia kwi-razor clam I-Sinonovacula constricta ngophando lwe-genome-wide association',Inkcubeko yasemanzini, 569, iphe. 739351. doi: 10.1016/J.AQUACULTURE.2023.739351.
Li, X. et al. (2022) 'Uhlalutyo lwe-Multi-omics lwe-398 ye-foxtail millet accessions lubonisa imimandla ye-genomic ehambelana nokufuywa ekhaya, iimpawu ze-metabolite, kunye nemiphumo yokuchasana nokudumba',Isityalo seemolekyuli, 15(8), iphepha 1367–1383. doi: 10.1016/j.molp.2022.07.003.
Li, J. et al. (2022) 'Genome-Wide Association Mapping of Hulless Barely Phenotypes kwindawo yembalela',Imida kwiNzululwazi yeZityalo, 13, iphe. 924892. doi: 10.3389/FPLS.2022.924892/BIBTEX.
Zhao, X. et al. (2021) 'I-GmST1, efaka i-sulfotransferase, inika ukuxhathisa kwiintsholongwane ze-soybean mosaic virus i-G2 kunye ne-G3',Isityalo, iSeli kunye nokusiNgqongileyo, 44(8), iphepha 2777-2792. doi: 10.1111/PCE.14066.
