Uhlalutyo lwe-Eukaryotic mRNA (IiReferensi esekwe kunye nokhetho lwe-de novo lukhoyo)
Lo mbhobho usebenzisa idatha ye-NGS RNA-Seq njengegalelo kwaye ivelise iziphumo ezivela kuhlalutyo oluninzi olusezantsi olubandakanya kodwa lungaphelelanga: ukulandelelana kovavanyo lomgangatho wedatha,de novoindawo yoshicilelo inkcazo, uhlalutyo oluguquguqukayo lokudibanisa, uhlalutyo lwentetho eyahlukileyo, inkcazo yomsebenzi kunye nohlalutyo lokutyebisa.
Uhlalutyo lwe-RNA olude olungakhowudi
Ii-RNA ezinde ezingezizo iikhowudi (lncRNA) ziyimibhalo engabhalwanga kunye nobude obude kune-200 nt kwaye iyaziwa ngokudlala indima kwintlangano ye-chromatin kunye nommiselo. Itekhnoloji yokulandelelaniswa komgangatho ophezulu kunye ne-bioinformatic ziye zaxhobisa ukuqonda kwethu ukulandelelana kwe-lncRNA kunye nokubeka ulwazi ukuchonga ii-lncRNA ezinemisebenzi ebalulekileyo yokulawula. Lo mbhobho ubonelela ngohlalutyo lwe-lncRNA ukongeza kuhlalutyo olukhankanywe kumbhobho we-Eukaryotic mRNA Analysis.
16S/18S/ITS ulandelelwano lweamplicon
Umbhobho wohlalutyo lwe-Amplicon Sequencing microbial diversity waphuhliswa ngokusekelwe kwiminyaka yamava kuhlalutyo lweprojekthi yokwahlukana kwemicrobial. Umbhobho uqulethe uhlalutyo olusisiseko olusemgangathweni, olubandakanya umxholo wohlalutyo oluqhelekileyo lophando lwangoku lwe-microbial kunye nohlalutyo lomntu. Ingxelo yohlalutyo ityebile kwaye ibanzi, kunye nokukhetha ukwenza uhlalutyo lomntu siqu. Ukongeza, iisampulu kunye namaqela anokuguqulwa kwi-fly ukuze kwenziwe ngokwezifiso ezongezelelweyo kunye nolawulo.
Shotgun Metagenomics Uhlalutyo
Umbhobho we-Shotgun Metagenomic Analysis usebenzisa idatha ye-NGS kwizinto ezixubileyo ze-genomic ezithathwe kwiisampuli zendalo. Uhlalutyo olubandakanyiweyo lubonelela ngolwazi olucacileyo malunga nokuhlukahluka kweentlobo kunye nobuninzi, ubume boluntu, ubudlelwane be-phylogenetic, izakhi zofuzo ezisebenzayo, kunye nothungelwano oluhambelanayo kunye nezinto ezisingqongileyo.
Uhlalutyo oluguquguqukayo lwe-NGS-WGS
Uhlalutyo lwe-NGS-WGS oluguquguqukayo luyimibhobho yokufumanisa edibeneyo eyenza ulawulo lomgangatho wedatha, ulandelelwano lolandelelwano, inkcazo, kunye nohlalutyo lokuguqulwa kofuzo. Umbhobho ulandela izenzo ezizezona ze-GATK ze-SNP kunye ne-InDel zokubona kwaye isebenzisa i-Manta kufowuno olwahlukileyo lwesakhiwo.
iGenome Wide Association Study (GWAS)
Umbhobho we-GWAS luhlalutyo olusezantsi oluthatha iifayile zeVCF ezenziwe ngaphambili kunye nedatha ye-phenotype ehambelanayo kwiqela labantu njengegalelo. Isebenzisa iindlela zobalo ezithile, i-GWAS ijolise ekutyhileni i-genome-wide nucleotide variations ehambelana ne-phenotypic differences. Idlala indima ebalulekileyo ekuphononongeni imizila yemfuza esebenzayo eyayanyaniswa nezifo ezintsonkothileyo zabantu kunye neempawu ezintsonkothileyo kwizityalo nakwizilwanyana.
Uhlalutyo loKwahlulwa koBuninzi (BSA)
Uhlalutyo lwe-BSA lubandakanya ukudibanisa abantu abaneempawu zephenotypic ezigqithisileyo ukusuka kuluntu olwahlulayo. Ngokuthelekisa i-loci yokwahlula phakathi kweesampulu ezidityanisiweyo, le ndlela ichonga ngokukhawuleza iziphawuli zeemolekyuli ezinxulunyaniswa nofuzo ekujoliswe kulo. Isetyenziswa kakhulu kwimaphu yofuzo yezityalo nezilwanyana, sisixhobo esixabisekileyo soncedo oluphawulweyo lokuzalanisa.
Uhlalutyo lwe-Evolutionary Genetics
Ucazululo lwe-Evolutionary Genetics Workflow ihambisa amava abanzi e-BMKGENE kwiiprojekthi zendaleko yemfuza kwaye ibandakanya ulwakhiwo lwemithi yephylogenetic, uhlalutyo lokungalungelelani konxibelelwano, uvavanyo lolwahlulo lwemfuza, ukuchongwa okukhethiweyo, uhlalutyo lokuzalana, uhlalutyo lwecandelo eliphambili, kunye nokubonakaliswa kobume babemi.
