TGuide Smart Magnetic Plant RNA Kit
Inombolo yekhathalogu / ukupakishwa
| Ikati. Hayi | ID | Inani lamalungiselelo |
| 4993552(ikhatriji) | DP662-DE | 48 |
Isikhokelo sokukhetha i-Reagent ye-TGuide S16
| Nuclei Acid | Uhlobo lweSampuli | Igama lemveliso | Ubungakanani bokupakisha (preps) Ikhatriji/ ipleyiti | Inombolo yekati. Ikhatriji/ipleyiti |
| DNA | Inyama yezilwanyana | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Isityalo kunye neMbewu | TGuide Smart Magnetic Plant DNA Kit | 48 | 4993548 |
| DNA | Umhlaba/Isitulo | TGuide Smart Soil / Stool DNA Kit | 48 | 4993549 |
| DNA | Iimveliso zeGel & PCR | TGuide Smart DNA Ikhithi yokuCoca | 48 | 4993550 |
| DNA | IGazi Elipheleleyo | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Iseli/iSwab/Amabala omileyo, njalo njalo | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| I-RNA | Igazi/Iseli/Izicubu | TGuide Smart Blood/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| I-RNA | Isityalo kunye neMbewu | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | Ulwelo lwasimahla lweSeli (iSerum, njalo njalo) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Intshayelelo yemveliso
Hlambulula isivuno esiphezulu, ubunyulu obuphezulu, i-RNA esemgangathweni ophezulu ukusuka kwigazi / iseli / izicubu.
Akukho zixhobo ezinetyhefu ezifana nephenol/chloroform equlethweyo
I-RNA efunyenweyo inobunyulu obuphezulu kwaye ingasetyenziselwa ngokuthe ngqo ukukhangela i-chip, ukulandelelana kwe-high-throughput kunye nezinye iimvavanyo.
Iimbonakalo
Kulula kakhulu ukuyisebenzisa
Akukho msebenzi owongezelelweyo wokubhobhoza.
Umthamo we-elution unokuhlengahlengiswa njengoko kufuneka.
Ufakelo kunye nokusebenza kufuna uqeqesho oluncinci. Ngeenkqubo ezifakwe ngaphambili, khupha i-cartridge, khetha iprotocol kwaye usebenzise uvavanyo lwakho. Iinkqubo zikulungele ukusetyenziswa, kwaye ziyakwazi ukwenziwa.
Ii-reagents ezilayishwe kwangaphambili kunye nezinto ezisetyenziselwa ukulahla.
Ukuphathwa kweekhemikhali kuyapheliswa ukuya kwinqanaba elikhulu lokuqinisekisa ukhuseleko lokusebenza.
Iziphumo zeDemo
Ingcambu yengqolowa iyonke ukutsalwa kwe-RNA
Ubungakanani besampuli: 100 mg
Isampuli yonyango: ukugaya nge-nitrogen engamanzi okanye unyango lwe-homogenizer lwethishu ephantsi
Uxinzelelo lwejeli yeAgarose :2%(TAE)
Ukulayisha umthamo: 2 μl
Umakishi: Umakisha IV, TIANGEN
Iziphumo zovavanyo: usebenzisa i-TGuide S16 ephunyezwe nge-reagent 4993552 ukukhupha ngokuzenzekelayo i-RNA iyonke yeengcambu zengqolowa inesivuno esihle kunye nokucoceka okuphezulu. Kulo vavanyo, malunga ne-20 μg ye-nucleic acid ikhutshwe kwi-100 mg yeengcambu zengqolowa, kunye ne-OD260 / OD280 malunga ne-2.0 ~ 2.1, kunye ne-OD260 / OD230＞2.0.
Iyonke i-RNA yokukhutshwa kwiisampuli zezityalo ezahlukeneyo
Ubungakanani besampuli: 100 mg
Isampuli yonyango: i-homogenizer yobushushu obuphantsi
Ijeli yeAgarose :1% (TAE)
Ukulayisha umthamo: 1 μl
M: Umakisha III, TIANGEN
1-4: imbewu yombona 5-8: amagqabi ombona 9-12: iingcambu zekhaphetshu 13-16: iimbotyi zesoya
Iisampulu ezimbini zokuqala zikhutshwe ngekhithi ye-spin-column extraction kwaye iisampuli ezimbini zokugqibela zikhutshwe yi-TGuide S16.
Iziphumo zovavanyo: isivuno sokutsalwa kwesityalo se-RNA yi-TGuide S16 ene-reagent 4993552 ilingana okanye ingcono kunaleyo ngekhithi yokutsalwa kwekholamu ye-spin. Ubunyulu nengqibelelo ziyafana. Ngaloo ndlela, i-TGuide S16 ingasetyenziselwa ukukhupha i-RNA yesityalo esipheleleyo endaweni yesisombululo se-spin-column based extraction.
