TGuide Smart Blood/Cell/Tissue RNA Kit
Inombolo yekhathalogu / ukupakishwa
| Ikati. Hayi | ID | Inani lamalungiselelo |
| 4995043(ipleyiti) | DP661-E | 96 |
| 4993551(ikhatriji) | DP661-DE | 48 |
Isikhokelo sokukhetha i-Reagent ye-TGuide S16
| Nuclei Acid | Uhlobo lweSampuli | Igama lemveliso | Ubungakanani bokupakisha (preps) Ikhatriji/ ipleyiti | Inombolo yekati. Ikhatriji/ipleyiti |
| DNA | Inyama yezilwanyana | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Isityalo kunye neMbewu | TGuide Smart Magnetic Plant DNA Kit | 48 | 4993548 |
| DNA | Umhlaba/Isitulo | TGuide Smart Soil / Stool DNA Kit | 48 | 4993549 |
| DNA | Iimveliso zeGel & PCR | TGuide Smart DNA Ikhithi yokuCoca | 48 | 4993550 |
| DNA | IGazi Elipheleleyo | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Iseli/iSwab/Amabala omileyo, njalo njalo | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| I-RNA | Igazi/Iseli/Izicubu | TGuide Smart Blood/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| I-RNA | Isityalo kunye neMbewu | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | Ulwelo lwasimahla lweSeli (iSerum, njalo njalo) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Intshayelelo yemveliso
Hlambulula isivuno esiphezulu, ubunyulu obuphezulu, i-RNA esemgangathweni ophezulu ukusuka kwigazi / iseli / izicubu.
Akukho zixhobo ezinetyhefu ezifana nephenol/chloroform equlethweyo
I-RNA efunyenweyo inobunyulu obuphezulu kwaye ingasetyenziselwa ngokuthe ngqo ukukhangela i-chip, ukulandelelana kwe-high-throughput kunye nezinye iimvavanyo.
Iimbonakalo
Kulula kakhulu ukuyisebenzisa
Akukho msebenzi owongezelelweyo wokubhobhoza.
Umthamo we-elution unokuhlengahlengiswa njengoko kufuneka.
Ufakelo kunye nokusebenza kufuna uqeqesho oluncinci. Ngeenkqubo ezifakwe ngaphambili, khupha i-cartridge, khetha iprotocol kwaye usebenzise uvavanyo lwakho. Iinkqubo zikulungele ukusetyenziswa, kwaye ziyakwazi ukwenziwa.
Ii-reagents ezilayishwe kwangaphambili kunye nezinto ezisetyenziselwa ukulahla.
Ukuphathwa kweekhemikhali kuyapheliswa ukuya kwinqanaba elikhulu lokuqinisekisa ukhuseleko lokusebenza.
Iziphumo zeDemo
I-Agilent 2100 yayisetyenziselwa ukufumanisa ingqibelelo.
Umthombo wesampula: CD-1 iimpuku
Ubume besampulu: igazi elitsha elipheleleyo
Ubungakanani besampuli: 200 μl
Isiphumo sovavanyo: i-RNA iyonke ekhutshwe egazini yi-TGuide S16 exhotywe nge-reagent 4993551 inesivuno esihle kunye nokucoceka okuphezulu, kwaye inokuhlangabezana neemfuno zezantsi.
ulandelelwano oluphezulu.
Umthombo wesampula: CD-1 iimpuku
Ukulayisha umthamo: 1 μl. Isibindi sihlanjululwe ngamaxesha angama-5 kwaye salayishwa.
I-Agarose gel concentration: 1%. I-Electrophoresis yemizuzu engama-20 kwi-6 V / cm;
M:TIANGEN uMphawu III 1-4: intliziyo 5-8:
isibindi 9-12: umphunga 13-16: izintso
Iisampulu ezimbini zokuqala zezicubu nganye zikhutshwe nge-spin-column based extraction kit, kwaye ezimbini zokugqibela zikhutshwe ngokuzenzekelayo yi-TGuide S16.
Umphumo wovavanyo: i-RNA yezilwanyana ezipheleleyo ezikhutshwe ngokuzenzekelayo yi-TGuide S16 exhotywe nge-reagent 4993551 inesivuno esiphezulu kunye nococeko, olulingana ne-RNA ekhutshwe yi-spin-column based extraction protocol. Ubunyulu ngokusisiseko bufana, kunye ne-OD260 / OD280 malunga ne-2.0 ~ 2.1 kunye ne-OD260 / OD230> 2.0. Ukuqukumbela, i-TGuide S16 ingasetyenziselwa ukukhupha i-RNA iyonke kwizicubu zezilwanyana endaweni yesisombululo se-spin-column based extraction.
